2024
A substitution at the cytoplasmic tail of the spike protein enhances SARS-CoV-2 infectivity and immunogenicity
Li Y, Zhang X, Tai W, Zhuang X, Shi H, Liao S, Yu X, Mei R, Chen X, Huang Y, Liu Y, Liu J, Liu Y, Zhu Y, Wang P, Tian M, Yu G, Li L, Cheng G. A substitution at the cytoplasmic tail of the spike protein enhances SARS-CoV-2 infectivity and immunogenicity. EBioMedicine 2024, 110: 105437. PMID: 39531918, DOI: 10.1016/j.ebiom.2024.105437.Peer-Reviewed Original ResearchS proteinCytoplasmic tailFERM-binding motifTrans-complementation systemSpike proteinAmino acid substitutionsProline-to-leucine substitutionSystematic bioinformatics analysisHigh-frequency mutationsSARS-CoV-2 virionsBinding motifAcid substitutionsSARS-CoV-2Natural selectionBioinformatics analysisEzrin/radixin/moesin proteinsMolecular mechanismsMutationsOmicron variantProteinVaccine developmentMRNA vaccinesGlobal disseminationSARS-CoV-2 Omicron sublineagesSublineagesAn evolutionarily conserved ubiquitin ligase drives infection and transmission of flaviviruses
Wu L, Zhang L, Feng S, Chen L, Lin C, Wang G, Zhu Y, Wang P, Cheng G. An evolutionarily conserved ubiquitin ligase drives infection and transmission of flaviviruses. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2317978121. PMID: 38593069, PMCID: PMC11032495, DOI: 10.1073/pnas.2317978121.Peer-Reviewed Original ResearchConceptsNonstructural proteinsSingle-stranded RNA genomeER-associated degradationE3 ligase HRD1Individual functional proteinsFlavivirus infectionCellular fitnessRNA genomeUbiquitin systemFlavivirus NS4AFunctional proteinsFlavivirus proteinsLysine residuesMammalian hostsMosquito-borne flavivirusSmall molecule inhibitorsStructural proteinsHrd1Viral proteinsProgeny virionsUbiquitinPotential therapeutic targetDENV2 infectionProteinNS4AA mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission
Wang Z, Nie K, Liang Y, Niu J, Yu X, Zhang O, Liu L, Shi X, Wang Y, Feng X, Zhu Y, Wang P, Cheng G. A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission. The EMBO Journal 2024, 43: 1690-1721. PMID: 38378891, PMCID: PMC11066113, DOI: 10.1038/s44318-024-00056-x.Peer-Reviewed Original ResearchMyeloid cellsExpression of neutrophil chemoattractantsViral transmissionInflux of myeloid cellsMosquito bite siteSalivary componentsInflux of neutrophilsSkin-resident macrophagesPotential prophylactic targetsFlavivirus transmissionNF-kB signalingMechanism of actionProphylactic targetsSystemic disseminationRecruits proteinsSalivary factorsLocal infectionNeutrophil chemoattractantMammalian hostsEfficient viral transmissionPhytochemical resveratrolDengue virusBite siteTransmission of ZikaProtein
2022
The noncanonical inflammasome in health and disease
Cahoon J, Yang D, Wang P. The noncanonical inflammasome in health and disease. Infectious Medicine 2022, 1: 208-216. PMID: 38077630, PMCID: PMC10699704, DOI: 10.1016/j.imj.2022.09.001.Peer-Reviewed Original ResearchNoncanonical inflammasomeRapid cellular responsesKey cellular regulatorsInnate immune signalingMechanism of activationAdaptor proteinCellular regulatorsPattern recognition receptorsNon-canonical inflammasomeImmune signalingCaspase-4Cellular responsesRecognition receptorsSubsequent maturationNegative bacterial infectionsCaspase-1Inflammatory cytokinesInflammatory diseasesInflammasomeBacterial infectionsRecent advancesDiseaseSignalingRegulatorProtein
2021
A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host
Yu X, Shan C, Zhu Y, Ma E, Wang J, Wang P, Shi P, Cheng G. A mutation-mediated evolutionary adaptation of Zika virus in mosquito and mammalian host. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2113015118. PMID: 34620704, PMCID: PMC8545446, DOI: 10.1073/pnas.2113015118.Peer-Reviewed Original ResearchConceptsEvolutionary adaptationZIKV capsid proteinAsian ZIKV lineageZika virusMammalian hostsZIKV mutantsNS2B-NS3 proteaseStructural proteinsPreferred substrateHuman cellsCapsid proteinInfectious viral particlesMutantsMosquito vectorsZIKV disseminationMillions of infectionsZIKV lineagesProteinViral particlesHigh prevalenceImmunodeficient miceMosquito infectionExplosive outbreaksMosquitoesStable substitutionsUBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.
Ketkar H, Harrison A, Graziano V, Geng T, Yang L, Vella A, Wang P. UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling. The Journal Of Immunology 2021, 206: 2682-2691. PMID: 34021047, PMCID: PMC8164993, DOI: 10.4049/jimmunol.1901337.Peer-Reviewed Original ResearchConceptsTyrosine kinase 2Domain-containing proteinsExpression of hundredsComplex cellular regulationRNA viral replicationJAK/STATCellular regulationType I/III IFNsKinase 2JAK/Type I/III IFNProtein 6IFN expressionGenesViral infectionSignalingExpressionViral replicationExpression of IFNCellsType IProteinSTATOverexpressionDeletion
2020
A mosquito salivary protein promotes flavivirus transmission by activation of autophagy
Sun P, Nie K, Zhu Y, Liu Y, Wu P, Liu Z, Du S, Fan H, Chen CH, Zhang R, Wang P, Cheng G. A mosquito salivary protein promotes flavivirus transmission by activation of autophagy. Nature Communications 2020, 11: 260. PMID: 31937766, PMCID: PMC6959235, DOI: 10.1038/s41467-019-14115-z.Peer-Reviewed Original ResearchConceptsBeclin-1Viral transmissionFlavivirus transmissionMosquito salivary proteinsHost immune cellsZika virus transmissionActivation of autophagyLow viremiaProphylactic targetsMosquito salivaImmune cellsZIKV transmissionAllergen 1Infected mosquitoesViral infectionMonocyte lineageVirus transmissionMiceMosquitoesSalivary proteinsNumerous studiesViremiaInfectionFlavivirusesProtein