2011
MEK5 is Activated by Shear Stress, Activates ERK5 and Induces KLF4 to Modulate TNF Responses in Human Dermal Microvascular Endothelial Cells
CLARK PR, JENSEN TJ, KLUGER MS, MORELOCK M, HANIDU A, QI Z, TATAKE RJ, POBER JS. MEK5 is Activated by Shear Stress, Activates ERK5 and Induces KLF4 to Modulate TNF Responses in Human Dermal Microvascular Endothelial Cells. Microcirculation 2011, 18: 102-117. PMID: 21166929, PMCID: PMC3075844, DOI: 10.1111/j.1549-8719.2010.00071.x.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCells, CulturedEndothelial CellsEnzyme ActivationGene Expression ProfilingGene Knockdown TechniquesHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMAP Kinase Kinase 5Mitogen-Activated Protein Kinase 7Oligonucleotide Array Sequence AnalysisRNA, MessengerRNA, Small InterferingShear StrengthStress, MechanicalTumor Necrosis Factor-alphaConceptsInduction of KLF4Inflammatory responseKLF4 expressionTNF responseInhibits Inflammatory ResponsesPro-inflammatory changesAnti-inflammatory pathwayICAM-1 expressionHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsMicrovascular endothelial cellsMicrovascular ECsWestern blottingEndothelial cellsRole of ERK5Protein expressionQRT-PCRSiRNA knockdownERK5 phosphorylationHDMECPrincipal siteHuman skinERK5 activationImmunofluorescence microscopyInduction
2007
Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect
Clark PR, Pober JS, Kluger MS. Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect. Nucleic Acids Research 2007, 36: 1081-1097. PMID: 18096615, PMCID: PMC2275081, DOI: 10.1093/nar/gkm630.Peer-Reviewed Original ResearchConceptsTumor necrosis factor receptor 1Endothelial cellsIntercellular adhesion molecule-1ICAM-1 siRNAAnti-TNF activityCultured human umbilical vein endothelial cellsICAM-1 expressionAdhesion molecule-1Off-target effectsNecrosis factor receptor 1Human umbilical vein ECHuman umbilical vein endothelial cellsFactor receptor 1Umbilical vein endothelial cellsExpression of mRNAIL-1 activationVein endothelial cellsLocal inflammationTNFR1 mRNANecrosis factorEarly TNFICAM-1VCAM-1E-selectinTransfection of ECs
2006
Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations
Clark PR, Manes TD, Pober JS, Kluger MS. Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations. Journal Of Investigative Dermatology 2006, 127: 762-774. PMID: 17195014, DOI: 10.1038/sj.jid.5700670.Peer-Reviewed Original ResearchConceptsEC barrier functionCell junctionsHuman dermal microvascular ECsSmall-interfering RNA knockdownEC shape changesEndothelial cellsG actin ratioActin cytoskeletonShape changesCytoskeleton organizationCell elongationICAM-1 inductionCytoskeletal reorganizationIntracellular regionCell shapeRNA knockdownHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsZona occludens-1NF-kappaB activationJunctional alterationsCytoskeletonBarrier functionCell leakinessMicrovascular endothelial cells