Paul Clark, PhD
Associate Research Scientist in GeneticsCards
Appointments
Genetics
Primary
Contact Info
Yale School of Medicine
WC ISTC Rm320, 850 West Campus Dr
West Haven, CT 06516
United States
About
Titles
Associate Research Scientist in Genetics
Appointments
Genetics
Associate Research ScientistPrimary
Other Departments & Organizations
- Genetics
Education & Training
- PhD
- University of Arizona (1999)
- BS
- University of Arizona (1985)
Research
Research at a Glance
Yale Co-Authors
Frequent collaborators of Paul Clark's published research.
Publications Timeline
A big-picture view of Paul Clark's research output by year.
Alexey Bersenev, MD, PhD
Jordan Pober, MD, PhD
Paula Preston-Hurlburt
Ruth R Montgomery, PhD
Sidi Chen, PhD
9Publications
397Citations
Publications
2022
A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy
Ye L, Park JJ, Peng L, Yang Q, Chow RD, Dong MB, Lam SZ, Guo J, Tang E, Zhang Y, Wang G, Dai X, Du Y, Kim HR, Cao H, Errami Y, Clark P, Bersenev A, Montgomery RR, Chen S. A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy. Cell Metabolism 2022, 34: 595-614.e14. PMID: 35276062, PMCID: PMC8986623, DOI: 10.1016/j.cmet.2022.02.009.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCAR T cellsT cell-based immunotherapyRight molecular targetCell-based immunotherapyCAR-T therapyChimeric antigen receptorMultiple cancer modelsCAR-T efficacyFunction CRISPR screensCD8 TPrimary CD8Immune functionImmunological diseasesImmune boosterCancer modelAntigen receptorDistinct gene expressionMolecular targetsCRISPR activation screensMetabolic programsImmunological analysisTherapyCancerEfficacyActivation screens
2020
Phosphoinositide 3′-Kinase γ Facilitates Polyomavirus Infection
Clark P, Gee GV, Albright BS, Assetta B, Han Y, Atwood WJ, DiMaio D. Phosphoinositide 3′-Kinase γ Facilitates Polyomavirus Infection. Viruses 2020, 12: 1190. PMID: 33092168, PMCID: PMC7589550, DOI: 10.3390/v12101190.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCRISPR/Cas9-mediated knockoutGenetic knockdown approachesCas9-mediated knockoutDNA tumor virusesProgressive multifocal leukoencephalopathyInfection defectPolyomavirus infectionCellular proteinsKnockdown approachSVG-A cellsPolyomavirus entryMolecular eventsVirus internalizationTumor virusPI3Kγ pathwayPIK3R5PI3KγGlial cellsKnockoutMultifocal leukoencephalopathyImmunosuppressed peopleCellsHuman polyomavirusesJC polyomavirusPolyomavirus
2016
The Receptor for Advanced Glycation Endproducts Drives T Cell Survival and Inflammation in Type 1 Diabetes Mellitus
Durning SP, Preston-Hurlburt P, Clark PR, Xu D, Herold KC, Group T. The Receptor for Advanced Glycation Endproducts Drives T Cell Survival and Inflammation in Type 1 Diabetes Mellitus. The Journal Of Immunology 2016, 197: 3076-3085. PMID: 27655844, PMCID: PMC5101164, DOI: 10.4049/jimmunol.1600197.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAdolescentAdultAsymptomatic DiseasesCD8-Positive T-LymphocytesCell SurvivalCells, CulturedChildDiabetes Mellitus, Type 1Disease ProgressionFemaleGene Expression ProfilingHumansImmunologic MemoryInflammationLymphocyte ActivationMaleReceptor for Advanced Glycation End ProductsRiskSignal TransductionUp-RegulationYoung AdultConceptsDamage-associated molecular patternsT cellsRAGE expressionT1D patientsInflammatory functionsRisk relativesCell activationHigh mobility group box 1Mobility group box 1Advanced glycated endproductsChronic autoimmune responseMolecular patternsEffector memory cellsHealthy control subjectsExpression of RAGEGroup box 1Type 1 diabetesAdvanced glycation endproductsT cell survivalAutoimmune responseAutoimmune diseasesControl subjectsDisease onsetRisk subjectsCell injury
2015
Tumor Necrosis Factor Disrupts Claudin-5 Endothelial Tight Junction Barriers in Two Distinct NF-κB-Dependent Phases
Clark PR, Kim RK, Pober JS, Kluger MS. Tumor Necrosis Factor Disrupts Claudin-5 Endothelial Tight Junction Barriers in Two Distinct NF-κB-Dependent Phases. PLOS ONE 2015, 10: e0120075. PMID: 25816133, PMCID: PMC4376850, DOI: 10.1371/journal.pone.0120075.Peer-Reviewed Original ResearchCitationsMeSH KeywordsCell Membrane PermeabilityCells, CulturedClaudin-5DermisEndothelium, VascularHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMyosin Light ChainsMyosin-Light-Chain KinaseNF-kappa BPhosphorylationRho-Associated KinasesRNA, Small InterferingSignal TransductionTight JunctionsTumor Necrosis Factor-alpha
2011
MEK5 is Activated by Shear Stress, Activates ERK5 and Induces KLF4 to Modulate TNF Responses in Human Dermal Microvascular Endothelial Cells
CLARK PR, JENSEN TJ, KLUGER MS, MORELOCK M, HANIDU A, QI Z, TATAKE RJ, POBER JS. MEK5 is Activated by Shear Stress, Activates ERK5 and Induces KLF4 to Modulate TNF Responses in Human Dermal Microvascular Endothelial Cells. Microcirculation 2011, 18: 102-117. PMID: 21166929, PMCID: PMC3075844, DOI: 10.1111/j.1549-8719.2010.00071.x.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsMeSH KeywordsBase SequenceCells, CulturedEndothelial CellsEnzyme ActivationGene Expression ProfilingGene Knockdown TechniquesHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMAP Kinase Kinase 5Mitogen-Activated Protein Kinase 7Oligonucleotide Array Sequence AnalysisRNA, MessengerRNA, Small InterferingShear StrengthStress, MechanicalTumor Necrosis Factor-alphaConceptsInduction of KLF4Inflammatory responseKLF4 expressionTNF responseInhibits Inflammatory ResponsesPro-inflammatory changesAnti-inflammatory pathwayICAM-1 expressionHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsMicrovascular endothelial cellsMicrovascular ECsWestern blottingEndothelial cellsRole of ERK5Protein expressionQRT-PCRSiRNA knockdownERK5 phosphorylationHDMECPrincipal siteHuman skinERK5 activationImmunofluorescence microscopyInduction
2007
Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect
Clark PR, Pober JS, Kluger MS. Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect. Nucleic Acids Research 2007, 36: 1081-1097. PMID: 18096615, PMCID: PMC2275081, DOI: 10.1093/nar/gkm630.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsTumor necrosis factor receptor 1Endothelial cellsIntercellular adhesion molecule-1ICAM-1 siRNAAnti-TNF activityCultured human umbilical vein endothelial cellsICAM-1 expressionAdhesion molecule-1Off-target effectsNecrosis factor receptor 1Human umbilical vein ECHuman umbilical vein endothelial cellsFactor receptor 1Umbilical vein endothelial cellsExpression of mRNAIL-1 activationVein endothelial cellsLocal inflammationTNFR1 mRNANecrosis factorEarly TNFICAM-1VCAM-1E-selectinTransfection of ECs
2006
Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations
Clark PR, Manes TD, Pober JS, Kluger MS. Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations. Journal Of Investigative Dermatology 2006, 127: 762-774. PMID: 17195014, DOI: 10.1038/sj.jid.5700670.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsEC barrier functionCell junctionsHuman dermal microvascular ECsSmall-interfering RNA knockdownEC shape changesEndothelial cellsG actin ratioActin cytoskeletonShape changesCytoskeleton organizationCell elongationICAM-1 inductionCytoskeletal reorganizationIntracellular regionCell shapeRNA knockdownHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsZona occludens-1NF-kappaB activationJunctional alterationsCytoskeletonBarrier functionCell leakinessMicrovascular endothelial cells
2001
The inducible Hsp70 as a marker of tumor immunogenicity.
Clark PR, Ménoret A. The inducible Hsp70 as a marker of tumor immunogenicity. Cell Stress & Chaperones 2001, 6: 121-5. PMID: 11599573, PMCID: PMC434389.Peer-Reviewed Original Research
1998
A novel drug screening assay for papillomavirus specific antiviral activity.
Clark PR, Roberts ML, Cowsert LM. A novel drug screening assay for papillomavirus specific antiviral activity. Antiviral Research 1998, 37: 97-106. PMID: 9588842.Peer-Reviewed Original Research
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TNFa Treatment of Human Dermal Microvascular Endothelial Cells (HDMEC) Leads to Intercellular Junction Disruption and Actin Stress Fiber Formation
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Yale School of Medicine
WC ISTC Rm320, 850 West Campus Dr
West Haven, CT 06516
United States