2002
Compensatory renal hypertrophy is mediated by a cell cycle-dependent mechanism
Liu B, Preisig PA. Compensatory renal hypertrophy is mediated by a cell cycle-dependent mechanism. Kidney International 2002, 62: 1650-1658. PMID: 12371965, DOI: 10.1046/j.1523-1755.2002.00620.x.Peer-Reviewed Original ResearchConceptsCdk2/cyclin E kinase activityCyclin E kinase activityTubule hypertrophyDay 4BrdU incorporationDevelopment of hypertrophyCyclin D kinaseCompensatory renal hypertrophyCell cycle-dependent mechanismProximal tubule proteinsTubule growthHypertrophic formUninephrectomized animalsNephrectomized ratsRenal hypertrophyC57BL6 miceRenal cortexKinase activityDay 2Proximal tubulesHypertrophyHypertrophy markersRatsKinase inhibitorsMice
2000
G1 kinases and transforming growth factor-β; signaling are associated with a growth pattern switch in diabetes-induced renal growth
Huang H, Preisig P. G1 kinases and transforming growth factor-β; signaling are associated with a growth pattern switch in diabetes-induced renal growth. Kidney International 2000, 58: 162-172. PMID: 10886561, DOI: 10.1046/j.1523-1755.2000.00151.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDC2-CDC28 KinasesCyclin ECyclin-Dependent Kinase 2Cyclin-Dependent Kinase 4Cyclin-Dependent KinasesDiabetes Mellitus, ExperimentalDiabetic NephropathiesDNA-Binding ProteinsG1 PhaseHyperplasiaHypertrophyKidney Tubules, ProximalMaleProtein Serine-Threonine KinasesProto-Oncogene ProteinsRatsRats, Sprague-DawleyReceptors, Transforming Growth Factor betaSignal TransductionSmad2 ProteinSmad4 ProteinTrans-ActivatorsTransforming Growth Factor betaConceptsReceptor II expressionCyclin E kinase activityReceptor expressionRenal growthGrowth factor beta receptor expressionTGF-beta receptor II expressionBaseline levelsProximal tubule cell growthTGF-beta receptor expressionBody weight ratioCdk2/cyclin E kinase activityCdk2/cyclin E complexesCyclin DRenal proximal tubulesDiabetes mellitusDiabetic ratsTGF-beta signalingDiabetic stateCyclin E complexGrowth patternDay 2Proximal tubulesDay 4Day 10Tubule growth
1999
A cell cycle-dependent mechanism of renal tubule epithelial cell hypertrophy
Preisig P. A cell cycle-dependent mechanism of renal tubule epithelial cell hypertrophy. Kidney International 1999, 56: 1193-1198. PMID: 10610409, DOI: 10.1046/j.1523-1755.1999.00708.x.Peer-Reviewed Original ResearchSize does matter: Will knockout of p21WAF1/CIP1 save the kidney by limiting compensatory renal growth?
Al-Awqati Q, Preisig P. Size does matter: Will knockout of p21WAF1/CIP1 save the kidney by limiting compensatory renal growth? Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 10551-10553. PMID: 10485857, PMCID: PMC33735, DOI: 10.1073/pnas.96.19.10551.Peer-Reviewed Original ResearchTGF-β1-mediated hypertrophy involves inhibiting pRB phosphorylation by blocking activation of cyclin E kinase
Liu B, Preisig P. TGF-β1-mediated hypertrophy involves inhibiting pRB phosphorylation by blocking activation of cyclin E kinase. American Journal Of Physiology 1999, 277: f186-f194. PMID: 10444572, DOI: 10.1152/ajprenal.1999.277.2.f186.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCdc25 PhosphatasesCDC2-CDC28 KinasesCell Cycle ProteinsCell LineCyclin ECyclin-Dependent Kinase 2Cyclin-Dependent Kinase 4Cyclin-Dependent Kinase Inhibitor p27Cyclin-Dependent Kinase Inhibitor p57Cyclin-Dependent KinasesEnzyme ActivationEpidermal Growth FactorHypertrophyKidneyMicrotubule-Associated ProteinsNuclear ProteinsPhosphorylationProtein Serine-Threonine KinasesProtein Tyrosine PhosphatasesProto-Oncogene ProteinsRatsRetinoblastoma ProteinTransforming Growth Factor betaTumor Suppressor ProteinsConceptsCdk2/cyclin E complexesCyclin E complexCyclin E kinaseGrowth responseE complexCell cycle progressionRenal epithelial cellsProtein abundanceRetinoblastoma proteinKinase activityCycle progressionHypophosphorylated statePRB phosphorylationHypertrophic growth responseKinaseCyclin EAbundanceEpithelial cellsActive stateDevelopment of hypertrophyActivationCell entranceComplexesArrestTGF-β1What Makes Cells Grow Larger and How Do They Do It? Renal Hypertrophy Revisited
Preisig P. What Makes Cells Grow Larger and How Do They Do It? Renal Hypertrophy Revisited. Nephron 1999, 7: 273-283. PMID: 10450014, DOI: 10.1159/000020614.Peer-Reviewed Original ResearchConceptsCell cycle-dependent mechanismCell cycle-independent mechanismsCell cycle processRegulation of processesRenal hypertrophyProtein degradationChronic potassium deficiencyCompensatory renal growthChronic metabolic acidosisDevelopment of hypertrophyCompensatory renal hypertrophyPotassium deficiencyDiabetes mellitusMetabolic acidosisLysosomal enzymesRenal growthCell sizeHypertrophyCell numberInitial eventProtein feedingRegulationVivo studiesCell culture studiesCycle process
1997
Media acidification inhibits TGF beta-mediated growth suppression in cultured rabbit proximal tubule cells.
Tovbin D, Franch HA, Alpern RJ, Preisig PA. Media acidification inhibits TGF beta-mediated growth suppression in cultured rabbit proximal tubule cells. Proceedings Of The Association Of American Physicians 1997, 109: 572-9. PMID: 9394419.Peer-Reviewed Original ResearchConceptsDirect antiproliferative effectRabbit proximal tubule cellsProximal tubule cellsAntiproliferative effectsGrowth-suppressive effectsMetabolic acidosisCultured rabbit proximal tubule cellsTubule cellsChronic metabolic acidosisInhibits cell cycle progressionRenal functionRenal growthMitogen-stimulated cellsRenal epithelial cellsProgressive lossHyperplasiaHypertrophyCell cycle progressionEpithelial cellsPrimary culturesPRB phosphorylationAcidosisEGF-induced phosphorylationGrowth suppressionCycle progression
1996
NH4Cl-induced hypertrophy is mediated by weak base effects and is independent of cell cycle processes
Franch H, Preisig P. NH4Cl-induced hypertrophy is mediated by weak base effects and is independent of cell cycle processes. American Journal Of Physiology 1996, 270: c932-c938. PMID: 8638677, DOI: 10.1152/ajpcell.1996.270.3.c932.Peer-Reviewed Original ResearchConceptsC-fos mRNA abundanceHuman papilloma virus 16NRK-52E cellsWeak base propertiesCell cycle processRenal hypertrophyClinical conditionsVirus 16Renal epithelial cellsCultured renal epithelial cellsCell cycleHypertrophyE7 genesAdditional studiesEpithelial cellsVesicular alkalinizationMRNA abundancePresent studyInactivation of pRbCellsProtein synthesisVacuolar proton pumpProtein abundanceVesicular compartments
1995
Renal epithelial cell hyperplasia and hypertrophy.
Preisig P, Franch H. Renal epithelial cell hyperplasia and hypertrophy. Seminars In Nephrology 1995, 15: 327-40. PMID: 7569412.Peer-Reviewed Original ResearchConceptsCell cycleNumber of proteinsCell hypertrophyRenal epithelial cellsDNA replicationProtein degradationCell protein contentCell growthGrowth responseCascade of eventsEpithelial cell hyperplasiaMitogenic stimuliIndependent mechanismsForm of hypertrophyEpithelial cellsSequential eventsLysosomal enzymesCurrent understandingPathological processesCell hyperplasiaProtein contentCellsKey roleEpithelial cell hypertrophyRate of growthInvolvement of pRB family in TGF beta-dependent epithelial cell hypertrophy.
Franch HA, Shay JW, Alpern RJ, Preisig PA. Involvement of pRB family in TGF beta-dependent epithelial cell hypertrophy. Journal Of Cell Biology 1995, 129: 245-254. PMID: 7698989, PMCID: PMC2120382, DOI: 10.1083/jcb.129.1.245.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, Polyomavirus TransformingBlotting, NorthernCell CycleCell DivisionCell LineCells, CulturedEpidermal Growth FactorEpithelial CellsEpitheliumGene ExpressionHumansHypertrophyKidneyKidney TubulesKineticsOncogene Proteins, ViralPapillomavirus E7 ProteinsPhosphorylationProto-Oncogene Proteins c-fosRabbitsRatsRecombinant ProteinsRepressor ProteinsRetinoblastoma ProteinRNA, MessengerTransfectionTransforming Growth Factor betaTumor Suppressor Protein p53ConceptsEpithelial cell hypertrophyNRK-52E cellsCell hypertrophyHPV16 E6TGF betaSV40 large T antigenC-fos mRNA abundanceGrowth factor beta 1SV40 large T antigen expressionLarge T antigenLarge T antigen expressionMechanisms of hypertrophyRenal cell hypertrophyRabbit proximal tubuleActive pRbT-antigen expressionRenal functionT antigenRenal hypertrophyAntigen expressionEpidermal growth factorEGF-induced increaseProximal tubulesHypertrophyHPV16 E7
1991
Increased Na/H antiporter and Na/3HCO3 symporter activities in chronic hyperfiltration. A model of cell hypertrophy.
Preisig PA, Alpern RJ. Increased Na/H antiporter and Na/3HCO3 symporter activities in chronic hyperfiltration. A model of cell hypertrophy. The Journal Of General Physiology 1991, 97: 195-217. PMID: 1849958, PMCID: PMC2216475, DOI: 10.1085/jgp.97.2.195.Peer-Reviewed Original ResearchConceptsHyperfiltration groupChronic hyperfiltrationNa/H antiporterKidney weightCell hypertrophyHigher glomerular filtration rateNa/H antiporter activityH antiporterGlomerular filtration rateVivo microperfused rat proximal tubuleCell pHRat proximal tubuleSymporter activityFiltration rateProximal tubulesUltrafiltrate-like solutionHyperfiltrationSteady-state cell pHProtein feedingPeritubular perfusateDietary proteinParallel increaseUninephrectomyHypertrophyCell acidification