2022
Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics
Shen B, Pade L, Choi S, Muñoz-LLancao P, Manzini M, Nemes P. Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics. Frontiers In Chemistry 2022, 10: 863979. PMID: 35464213, PMCID: PMC9024316, DOI: 10.3389/fchem.2022.863979.Peer-Reviewed Original ResearchHigh-resolution mass spectrometryCapillary electrophoresisElectrospray ionization (ESISingle-cell proteomicsMass spectrometryCapillary electrophoresis mass spectrometryNano-flow liquid chromatographySample preparationSingle-cell technologiesSample amountCapillary microsamplingSubcellular proteomicsCellular dimensionsLiquid chromatographyMidline cellsSpectrometryProteomicsSubcellular analysisProtein groupsProteinMouse hippocampusSerial dilutionProtein digestibilityESINeurons
2021
Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics
Choi S, Muñoz-LLancao P, Manzini M, Nemes P. Data-Dependent Acquisition Ladder for Capillary Electrophoresis Mass Spectrometry-Based Ultrasensitive (Neuro)Proteomics. Analytical Chemistry 2021, 93: 15964-15972. PMID: 34812615, DOI: 10.1021/acs.analchem.1c03327.Peer-Reviewed Original ResearchConceptsData-dependent acquisitionHigh-resolution mass spectrometryTraditional data-dependent acquisitionAnalytical toolbox of neuroscienceCE-ESIElectrospray ionizationUltrasensitive proteomicsDynamic exclusionMass spectrometryPeptide signalsReplicate measurementsTrace amountsNeuronal marker genesIdentification success rateAnalytical toolboxIdentified proteinsNonredundant proteinsData acquisition strategyMarker genesProtein digestibilityProteinNeurobiological importanceMolecular pathwaysCultured hippocampalIonization
2017
Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry
Choi S, Lombard-Banek C, Muñoz-LLancao P, Manzini M, Nemes P. Enhanced Peptide Detection Toward Single-Neuron Proteomics by Reversed-Phase Fractionation Capillary Electrophoresis Mass Spectrometry. Journal Of The American Society For Mass Spectrometry 2017, 29: 913-922. PMID: 29147852, DOI: 10.1007/s13361-017-1838-1.Peer-Reviewed Original ResearchConceptsHigh-resolution mass spectrometryReversed-phaseCapillary electrophoresisMass spectrometryTandem high-resolution mass spectrometryCapillary electrophoresis mass spectrometryRates of peptide identificationsRP fractionationCE separationProtein groupsTrace-level sensitivityRP columnDetected peptidesPeptide identificationProducts of genesSpectral interferencesProtein identificationMS/MSTechnical triplicatesCytoskeletal organizationSpectrometryIdentified proteinsCo-isolatedDetection sensitivityProtein digestibility