2004
The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway
Lewis L, Cylin E, Lee H, Saby R, Wong W, Grindley N. The Left End of IS2: a Compromise between Transpositional Activity and an Essential Promoter Function That Regulates the Transposition Pathway. Journal Of Bacteriology 2004, 186: 858-865. PMID: 14729714, PMCID: PMC321474, DOI: 10.1128/jb.186.3.858-865.2004.Peer-Reviewed Original ResearchBase SequenceDNA Transposable ElementsDNA, CircularMolecular Sequence DataPromoter Regions, GeneticTransposases
2002
Cryptic plasmids of Mycobacterium avium: Tn552 to the rescue
Kirby C, Waring A, Griffin T, Falkinham J, Grindley N, Derbyshire K. Cryptic plasmids of Mycobacterium avium: Tn552 to the rescue. Molecular Microbiology 2002, 43: 173-186. PMID: 11849545, DOI: 10.1046/j.1365-2958.2002.02729.x.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, SouthernDNA Transposable ElementsDNA, BacterialDNA, CircularMolecular Sequence DataMutagenesis, InsertionalMycobacterium aviumMycobacterium bovisMycobacterium smegmatisPlasmidsReplication OriginRestriction MappingSequence Analysis, DNASequence Homology, Nucleic AcidConceptsEssential genetic toolsCryptic plasmidGenetic toolsOpportunistic pathogen Mycobacterium aviumGenetic exploitationTransposon insertionConjugative relaxaseTransposition systemSelectable markerExtrachromosomal DNAGenetic analysisHost rangePlasmid genesPlasmid originBacterial speciesPlasmid establishmentCircular DNAPlasmidMycobacterium smegmatisGenesMycobacterial plasmidsDNAReplicationMycobacterium aviumRescue
2001
The basis of asymmetry in IS2 transposition
Lewis L, Gadura N, Greene M, Saby R, Grindley N. The basis of asymmetry in IS2 transposition. Molecular Microbiology 2001, 42: 887-901. PMID: 11737634, DOI: 10.1046/j.1365-2958.2001.02662.x.Peer-Reviewed Original ResearchIn Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni
Colegio O, Griffin T, Grindley N, Galán J. In Vitro Transposition System for Efficient Generation of Random Mutants of Campylobacter jejuni. Journal Of Bacteriology 2001, 183: 2384-2388. PMID: 11244083, PMCID: PMC95150, DOI: 10.1128/jb.183.7.2384-2388.2001.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCampylobacter jejuniDNA Transposable ElementsMolecular Sequence DataMutagenesis
2000
Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552′phoA In Vitro Transposition System
Braunstein M, Griffin T, Kriakov J, Friedman S, Grindley N, Jacobs W. Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn552′phoA In Vitro Transposition System. Journal Of Bacteriology 2000, 182: 2732-2740. PMID: 10781540, PMCID: PMC101980, DOI: 10.1128/jb.182.10.2732-2740.2000.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acid SequenceArtificial Gene FusionBacterial ProteinsBiological TransportCosmidsCyclin-Dependent KinasesDNA Transposable ElementsDNA, BacterialGenes, BacterialGenomic LibraryMolecular Sequence DataMutagenesis, InsertionalMycobacterium tuberculosisPlasmidsReplication OriginConceptsProtective immunityM. tuberculosisMycobacterium tuberculosis pathogenesisImmune responseTuberculosis pathogenesisMycobacterium tuberculosis proteinsM. tuberculosis databaseTuberculosisTuberculosis databasePathogenesisTuberculosis proteinsImmunityEnvelope-associated proteinsM. smegmatisProteinPeptides
1999
In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis
Griffin T, Leschziner A, Grindley N, Parsons L, DeVost J, Derbyshire K. In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis. Nucleic Acids Research 1999, 27: 3859-3865. PMID: 10481025, PMCID: PMC148649, DOI: 10.1093/nar/27.19.3859.Peer-Reviewed Original ResearchMeSH KeywordsDNA Transposable ElementsExodeoxyribonuclease VExodeoxyribonucleasesMolecular Sequence DataMutagenesis, InsertionalMycobacterium smegmatisSequence Analysis, DNATransposasesConceptsDNA sequencingTarget DNAEntire operonInsertion mutantsGene clusterGenetic toolsNucleotide sequenceSequence specificityUnique sequencesMolecular biologyTransposition reactionNovel transposonTransposonSequencingComprehensive collectionDNAArsenal of toolsSequenceOperonGenomicsMutantsCosmidsMutagenesisKbBiology
1998
Tn552 transposase catalyzes concerted strand transfer in vitro
Leschziner A, Griffin T, Grindley N. Tn552 transposase catalyzes concerted strand transfer in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 7345-7350. PMID: 9636151, PMCID: PMC22612, DOI: 10.1073/pnas.95.13.7345.Peer-Reviewed Original Research
1997
Two abundant intramolecular transposition products, resulting from reactions initiated at a single end, suggest that IS2 transposes by an unconventional pathway
Lewis L, Grindley N. Two abundant intramolecular transposition products, resulting from reactions initiated at a single end, suggest that IS2 transposes by an unconventional pathway. Molecular Microbiology 1997, 25: 517-529. PMID: 9302014, DOI: 10.1046/j.1365-2958.1997.4871848.x.Peer-Reviewed Original ResearchBacterial ProteinsBase SequenceBinding SitesCloning, MolecularDNA NucleotidyltransferasesDNA PrimersDNA Transposable ElementsDNA, BacterialDNA, CircularEscherichia coliEscherichia coli ProteinsMicroscopy, ElectronModels, GeneticMolecular Sequence DataNucleic Acid ConformationPolymerase Chain ReactionRecombinant Fusion ProteinsTransposases
1995
DNA transposition: From a black box to a color monitor
Grindley N, Leschziner A. DNA transposition: From a black box to a color monitor. Cell 1995, 83: 1063-1066. PMID: 8548793, DOI: 10.1016/0092-8674(95)90132-9.Peer-Reviewed Original ResearchDNA NucleotidyltransferasesDNA Transposable ElementsDNA-Binding ProteinsProtein ConformationTransposasesCatalytic residues of gamma delta resolvase act in cis.
Boocock M, Zhu X, Grindley N. Catalytic residues of gamma delta resolvase act in cis. The EMBO Journal 1995, 14: 5129-5140. PMID: 7588641, PMCID: PMC394616, DOI: 10.1002/j.1460-2075.1995.tb00195.x.Peer-Reviewed Original ResearchBase SequenceBinding SitesCrossing Over, GeneticDNA NucleotidyltransferasesDNA Topoisomerases, Type IDNA Transposable ElementsGenetic Complementation TestModels, GeneticModels, MolecularMolecular Sequence DataPlasmidsRecombination, GeneticStructure-Activity RelationshipSubstrate SpecificityTransposases
1992
Binding of the IS903 transposase to its inverted repeat in vitro.
Derbyshire K, Grindley N. Binding of the IS903 transposase to its inverted repeat in vitro. The EMBO Journal 1992, 11: 3449-3455. PMID: 1324175, PMCID: PMC556880, DOI: 10.1002/j.1460-2075.1992.tb05424.x.Peer-Reviewed Original Research
1991
Gamma delta transposase. Purification and analysis of its interaction with a transposon end
Wiater L, Grindley N. Gamma delta transposase. Purification and analysis of its interaction with a transposon end. Journal Of Biological Chemistry 1991, 266: 1841-1849. PMID: 1846366, DOI: 10.1016/s0021-9258(18)52370-x.Peer-Reviewed Original Research
1990
Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction
Hughes R, Hatfull G, Rice P, Steitz T, Grindley N. Cooperativity mutants of the γδ resolvase identify an essential interdimer interaction. Cell 1990, 63: 1331-1338. PMID: 2175679, DOI: 10.1016/0092-8674(90)90428-h.Peer-Reviewed Original ResearchConceptsProtein-protein interactionsHigher-order protein-protein interactionsCooperativity mutantsSite-specific recombinaseGamma delta resolvaseMutant phenotypeResolvase mutantsNucleoprotein complexesCrystallographic tetramersResolvase dimersΓδ resolvaseResolvaseCooperative bindingMutantsDNARecombinationSide chainsRecombinaseProteinInteractionCointegrate intermediatePhenotypeRecombination reactionBindingTetramerIntegration host factor increases the transpositional immunity conferred by gamma delta ends
Wiater L, Grindley N. Integration host factor increases the transpositional immunity conferred by gamma delta ends. Journal Of Bacteriology 1990, 172: 4951-4958. PMID: 2168370, PMCID: PMC213150, DOI: 10.1128/jb.172.9.4951-4958.1990.Peer-Reviewed Original ResearchUncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta
Wiater L, Grindley N. Uncoupling of transpositional immunity from gamma delta transposition by a mutation at the end of gamma delta. Journal Of Bacteriology 1990, 172: 4959-4963. PMID: 2168371, PMCID: PMC213151, DOI: 10.1128/jb.172.9.4959-4963.1990.Peer-Reviewed Original ResearchConceptsGamma deltaRole of instability in the cis action of the insertion sequence IS903 transposase.
Derbyshire K, Kramer M, Grindley N. Role of instability in the cis action of the insertion sequence IS903 transposase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4048-4052. PMID: 2161528, PMCID: PMC54044, DOI: 10.1073/pnas.87.11.4048.Peer-Reviewed Original ResearchConceptsDNA-binding proteinsCis actionCis-acting proteinsKilobase of DNAInsertion sequenceGalactosidase fusion proteinWild-type transposaseProtease-deficient strainSite of synthesisLon geneBacterial insertion sequencesUnstable proteinTransposase geneFusion proteinTrans actionTransposaseEscherichia coliProteinDefective transposonTransposonGenes
1988
Gamma delta transposase and integration host factor bind cooperatively at both ends of gamma delta.
Wiater L, Grindley N. Gamma delta transposase and integration host factor bind cooperatively at both ends of gamma delta. The EMBO Journal 1988, 7: 1907-1911. PMID: 2844529, PMCID: PMC457184, DOI: 10.1002/j.1460-2075.1988.tb03024.x.Peer-Reviewed Original Research
1987
Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats.
Derbyshire K, Hwang L, Grindley N. Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 8049-8053. PMID: 2825175, PMCID: PMC299474, DOI: 10.1073/pnas.84.22.8049.Peer-Reviewed Original ResearchThe γδ resolvase induces an unusual DNA structure at the recombinational crossover point
Hatfull G, Noble S, Grindley N. The γδ resolvase induces an unusual DNA structure at the recombinational crossover point. Cell 1987, 49: 103-110. PMID: 3030563, DOI: 10.1016/0092-8674(87)90760-4.Peer-Reviewed Original ResearchContacts between gamma delta resolvase and the gamma delta res site.
Falvey E, Grindley N. Contacts between gamma delta resolvase and the gamma delta res site. The EMBO Journal 1987, 6: 815-821. PMID: 3034611, PMCID: PMC553467, DOI: 10.1002/j.1460-2075.1987.tb04824.x.Peer-Reviewed Original ResearchAlkylationBase SequenceDNA Transposable ElementsMethylationNucleotidyltransferasesPlasmidsProtein BindingTransposases