2002
Cryptic plasmids of Mycobacterium avium: Tn552 to the rescue
Kirby C, Waring A, Griffin T, Falkinham J, Grindley N, Derbyshire K. Cryptic plasmids of Mycobacterium avium: Tn552 to the rescue. Molecular Microbiology 2002, 43: 173-186. PMID: 11849545, DOI: 10.1046/j.1365-2958.2002.02729.x.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, SouthernDNA Transposable ElementsDNA, BacterialDNA, CircularMolecular Sequence DataMutagenesis, InsertionalMycobacterium aviumMycobacterium bovisMycobacterium smegmatisPlasmidsReplication OriginRestriction MappingSequence Analysis, DNASequence Homology, Nucleic AcidConceptsEssential genetic toolsCryptic plasmidGenetic toolsOpportunistic pathogen Mycobacterium aviumGenetic exploitationTransposon insertionConjugative relaxaseTransposition systemSelectable markerExtrachromosomal DNAGenetic analysisHost rangePlasmid genesPlasmid originBacterial speciesPlasmid establishmentCircular DNAPlasmidMycobacterium smegmatisGenesMycobacterial plasmidsDNAReplicationMycobacterium aviumRescue
1994
Resolvase-Mediated Site-Specific Recombination
Grindley N. Resolvase-Mediated Site-Specific Recombination. Nucleic Acids And Molecular Biology 1994, 8: 236-267. DOI: 10.1007/978-3-642-78666-2_14.Peer-Reviewed Original ResearchSite-specific recombinationConservative site-specific recombinationPlasmid chromosomesFunctional genesPhage genomeDNA segmentsSynthesis of DNABiological roleGene expressionChromosomal integrationMost biological systemsGene cassettesTransposition intermediatesReciprocal exchangeRecombinationBiological systemsGenomeChromosomesResolvaseGenesDNACassetteMonomerizationExpression
1990
Role of instability in the cis action of the insertion sequence IS903 transposase.
Derbyshire K, Kramer M, Grindley N. Role of instability in the cis action of the insertion sequence IS903 transposase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4048-4052. PMID: 2161528, PMCID: PMC54044, DOI: 10.1073/pnas.87.11.4048.Peer-Reviewed Original ResearchConceptsDNA-binding proteinsCis actionCis-acting proteinsKilobase of DNAInsertion sequenceGalactosidase fusion proteinWild-type transposaseProtease-deficient strainSite of synthesisLon geneBacterial insertion sequencesUnstable proteinTransposase geneFusion proteinTrans actionTransposaseEscherichia coliProteinDefective transposonTransposonGenes
1985
Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli
Joyce C, Fujii D, Laks H, Hughes C, Grindley N. Genetic mapping and DNA sequence analysis of mutations in the polA gene of Escherichia coli. Journal Of Molecular Biology 1985, 186: 283-293. PMID: 3910840, DOI: 10.1016/0022-2836(85)90105-6.Peer-Reviewed Original ResearchConceptsDNA sequence analysisDNA polymerase IThree-dimensional structurePolymerase ISequence analysisPolA geneSingle-subunit enzymeEscherichia coliEnzyme-DNA interactionsGenetic mappingDeletion mutantsSubunit enzymeMutant formsPrimary sequenceMutational changesBacteriophage lambdaExcellent modelPolA mutantsPolA mutationEnzymatic behaviorMutantsMutationsGenesDNAColi
1984
Mutants of the γδ resolvase: A genetic analysis of the recombination function
Newman B, Grindley N. Mutants of the γδ resolvase: A genetic analysis of the recombination function. Cell 1984, 38: 463-469. PMID: 6088082, DOI: 10.1016/0092-8674(84)90501-4.Peer-Reviewed Original ResearchConceptsAmino acid amino-terminal domainAmino-terminal domainDifferent amino acid substitutionsSite-specific recombinationGamma delta transposonAmino acid substitutionsRecombination functionsTransposon genesIndependent mutantsCatalytic domainRelated recombinasesGenetic analysisDistinct residuesΓδ resolvaseMutantsAcid substitutionsResolvaseRegulatory propertiesCrossover sitesAmino acidsResolvase proteinCointegrate resolutionRecombinasesTransposonGenesMethod for determining whether a gene of Escherichia coli is essential: application to the polA gene
Joyce C, Grindley N. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. Journal Of Bacteriology 1984, 158: 636-643. PMID: 6233260, PMCID: PMC215477, DOI: 10.1128/jb.158.2.636-643.1984.Peer-Reviewed Original ResearchConceptsPolA geneTarget genesRich mediumEscherichia coliPhage lambda vectorTarget lociBacterial chromosomeHomologous recombinationPhysical mapProphage excisionDNA segmentsMinimal mediumAntibiotic resistance genesPolymerase 3Recombinational eventsLambda vectorGenesResistance genesChromosomal deletionsDeletionFunctional fragmentsExonucleaseMarked deletionPhagesPresence of plasmids
1982
Identification of two genes immediately downstream from the polA gene of Escherichia coli
Joyce C, Grindley N. Identification of two genes immediately downstream from the polA gene of Escherichia coli. Journal Of Bacteriology 1982, 152: 1211-1219. PMID: 6183253, PMCID: PMC221628, DOI: 10.1128/jb.152.3.1211-1219.1982.Peer-Reviewed Original ResearchNucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.
Joyce C, Kelley W, Grindley N. Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Journal Of Biological Chemistry 1982, 257: 1958-1964. PMID: 6276402, DOI: 10.1016/s0021-9258(19)68132-9.Peer-Reviewed Original ResearchConceptsDNA polymerase IPolymerase INucleotide sequencePolA geneKilobase pair regionProtein chemical dataAmino acid sequenceWild-type alleleResidues 342Sequence comparisonDNA polymerase I.Polymerase moleculesDNA sequencesResidue 323Acid sequencePair regionPolA1 mutationPolymerase I.Primary structureBase pairsType alleleMild proteolysisGenesActive fragmentSequence
1981
Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site
Reed R, Grindley N. Transposon-mediated site-specific recombination in vitro: DNA cleavage and protein-DNA linkage at the recombination site. Cell 1981, 25: 721-728. PMID: 6269756, DOI: 10.1016/0092-8674(81)90179-3.Peer-Reviewed Original ResearchConceptsSite-specific recombinationTransposable element gamma deltaDNA cleavageTnpR genePalindromic sequence 5'Intercistronic regionSequence 5DNA moleculesResolution siteResolvaseSame substrate moleculeRecombination sitesAbsence of Mg2Substrate moleculesGenesSame repliconRecombinationDNACleavageTransposonRes sitesSites
1980
THE PRIMARY STRUCTURE OF DNA POLYMERASE I OF E. COLI11This work was supported by Health and Research Services Foundation grant V-34 (to NDFG), NIH grant GM24688 (to WSK) and ACS Faculty Research Award 198 (to WSK).
Joyce C, Kelley W, Brown W, Grindley N. THE PRIMARY STRUCTURE OF DNA POLYMERASE I OF E. COLI11This work was supported by Health and Research Services Foundation grant V-34 (to NDFG), NIH grant GM24688 (to WSK) and ACS Faculty Research Award 198 (to WSK). 1980, 589-596. DOI: 10.1016/b978-0-12-048850-6.50054-5.Peer-Reviewed Original ResearchDNA polymerase IPolymerase IE. coli polA geneAmino acid sequenceHalf-cystine residuesProteolytic cleavage sitesResidues 342Sequence comparisonDNA polymerase I.DNA sequencesResidue 323Acid sequencePolymerase I.PolA geneNative enzymeCleavage siteAmino acidsSequenceGenesProteinMutationsAllelesEnzymeResiduesTrp
1976
polA6, a mutation affecting the DNA binding capacity of DNA polymerase I
Kelly W, Grindley N. polA6, a mutation affecting the DNA binding capacity of DNA polymerase I. Nucleic Acids Research 1976, 3: 2971-2984. PMID: 12497, PMCID: PMC343145, DOI: 10.1093/nar/3.11.2971.Peer-Reviewed Original ResearchMapping of thepolA locus ofEscherichia coli K12: Orientation of the amino- and carboxy-termini of the cistron
Kelley W, Grindley N. Mapping of thepolA locus ofEscherichia coli K12: Orientation of the amino- and carboxy-termini of the cistron. Molecular Genetics And Genomics 1976, 147: 307-314. PMID: 787765, DOI: 10.1007/bf00582882.Peer-Reviewed Original Research