2020
Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
Vogels CBF, Brito AF, Wyllie AL, Fauver JR, Ott IM, Kalinich CC, Petrone ME, Casanovas-Massana A, Catherine Muenker M, Moore AJ, Klein J, Lu P, Lu-Culligan A, Jiang X, Kim DJ, Kudo E, Mao T, Moriyama M, Oh JE, Park A, Silva J, Song E, Takahashi T, Taura M, Tokuyama M, Venkataraman A, Weizman OE, Wong P, Yang Y, Cheemarla NR, White EB, Lapidus S, Earnest R, Geng B, Vijayakumar P, Odio C, Fournier J, Bermejo S, Farhadian S, Dela Cruz CS, Iwasaki A, Ko AI, Landry ML, Foxman EF, Grubaugh ND. Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets. Nature Microbiology 2020, 5: 1299-1305. PMID: 32651556, PMCID: PMC9241364, DOI: 10.1038/s41564-020-0761-6.Peer-Reviewed Original ResearchConceptsSARS-CoV-2SARS-CoV-2 RTSevere acute respiratory syndrome coronavirusAcute respiratory syndrome coronavirusViral RNA copiesPublic health laboratoriesPublic health interventionsReverse transcription-PCR assaySARS-CoV-2 diagnostic testingDiagnostic assaysTranscription-PCR assaySARS-CoV-2 evolutionQuantitative reverse transcription-PCR assaysRapid diagnostic assaysHealth laboratoriesHealth interventionsDiagnostic testingRNA copiesPrimer-probe setsAssaysLow sensitivityCritical needAnalytical sensitivity
2016
The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion
Moriyama M, Chen I, Kawaguchi A, Koshiba T, Nagata K, Takeyama H, Hasegawa H, Ichinohe T. The RNA- and TRIM25-Binding Domains of Influenza Virus NS1 Protein Are Essential for Suppression of NLRP3 Inflammasome-Mediated Interleukin-1β Secretion. Journal Of Virology 2016, 90: 4105-4114. PMID: 26865721, PMCID: PMC4810543, DOI: 10.1128/jvi.00120-16.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCarrier ProteinsHEK293 CellsHeLa CellsHumansInflammasomesInterleukin-1betaMacrophagesMice, Inbred C57BLMitochondrial MembranesNLR Family, Pyrin Domain-Containing 3 ProteinRNATranscription FactorsTripartite Motif ProteinsUbiquitin-Protein LigasesViral Nonstructural ProteinsConceptsIL-1β secretionNonstructural protein 1Virus M2 proteinSuppression of NLRP3NS1 proteinNS1 RNAInfluenza virusM2 proteinIL-18Influenza virus NS1 proteinInterleukin-1βMultimolecular protein complexesProtein 1Inflammasome-mediated IL-1β secretionLike receptor family pyrinCaspase-1New anti-inflammatory drugsVirus NS1 proteinRecent pandemic strainJ774A.1 macrophagesPrecise mechanismNLRP3 inflammasome activationAnti-inflammatory drugsProtein complexesInnate immune sensing