2023
GOT1 constrains TH17 cell differentiation, while promoting iTreg cell differentiation
Xu W, Patel C, Alt J, Zhao L, Sun I, Oh M, Sun I, Wen J, Blosser R, Tam A, Powell J. GOT1 constrains TH17 cell differentiation, while promoting iTreg cell differentiation. Nature 2023, 614: e1-e11. PMID: 36726001, DOI: 10.1038/s41586-022-05602-3.Peer-Reviewed Original ResearchGOT1 regulates CD8+ effector and memory T cell generation
Xu W, Patel C, Zhao L, Sun I, Oh M, Sun I, Helms R, Wen J, Powell J. GOT1 regulates CD8+ effector and memory T cell generation. Cell Reports 2023, 42: 111987. PMID: 36640309, PMCID: PMC9943022, DOI: 10.1016/j.celrep.2022.111987.Peer-Reviewed Original ResearchMeSH KeywordsCD8-Positive T-LymphocytesCell DifferentiationGlucoseImmunologic MemoryMemory T CellsT-Lymphocytes, CytotoxicConceptsIntracellular redox balancePosttranslational regulationHIF proteinsMetabolic reprogrammingRedox balanceΑ-ketoglutarateGOT1T cell activationCell generationGenetic deletionEffector differentiationCritical roleCell activationDifferentiationRegulationProliferationReprogrammingBiosynthesisEffectorsProteinDeletionMechanisticallyCytotoxic functionFunctionPurine
2022
Cutting Edge: mTORC2 Regulates CD8+ Effector and Memory T Cell Differentiation through Serum and Glucocorticoid Kinase 1.
Patel C, Heikamp E, Xu W, Sun I, Oh M, Sun I, Wen J, Tam A, Blosser R, Powell J. Cutting Edge: mTORC2 Regulates CD8+ Effector and Memory T Cell Differentiation through Serum and Glucocorticoid Kinase 1. The Journal Of Immunology 2022, 209: 2287-2291. PMID: 36469844, PMCID: PMC10065985, DOI: 10.4049/jimmunol.2100669.Peer-Reviewed Original ResearchConceptsT cell differentiationT cellsMemory T cell differentiationMemory precursor phenotypeMemory T cellsEfficacy of vaccinesGlucocorticoid-regulated kinase 1Mechanistic targetRapamycin complex 2 (mTORC2) signalingT cell metabolismT cells resultsKinase 1Memory CD8Acute infectionGlucocorticoid kinase 1Tumor immunotherapyCD8Cell differentiationPrecursor phenotypeNuclear translocationPowerful targetSerumRecall capacityEssential regulatorCell metabolism
2018
mTOR Complex 1 Signaling Regulates the Generation and Function of Central and Effector Foxp3+ Regulatory T Cells
Sun I, Oh M, Zhao L, Patel C, Arwood M, Xu W, Tam A, Blosser R, Wen J, Powell J. mTOR Complex 1 Signaling Regulates the Generation and Function of Central and Effector Foxp3+ Regulatory T Cells. The Journal Of Immunology 2018, 201: 481-492. PMID: 29884702, PMCID: PMC6089237, DOI: 10.4049/jimmunol.1701477.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell DifferentiationFemaleForkhead Transcription FactorsImmunologic MemoryInducible T-Cell Co-Stimulator ProteinLymphocyte ActivationMaleMechanistic Target of Rapamycin Complex 1MiceProgrammed Cell Death 1 ReceptorRegulatory-Associated Protein of mTORSignal TransductionT-Lymphocytes, RegulatoryConceptsT cell activationT cellsCentral TregsGenetic deletionCell activationRegulatory T cell differentiationGeneration of TregsRegulatory T cellsEffector T cellsMemory-like phenotypeT cell differentiationInhibition of mTORSpare respiratory capacityEffector TregsRole of mTORPD-1Treg functionImmune microenvironmentMemory TregsTregsPharmacologic inhibitionDecreased expressionMammalian targetPharmacologic inhibitorsMTOR activity
2016
Asymmetric inheritance of mTORC1 kinase activity during division dictates CD8+ T cell differentiation
Pollizzi K, Sun I, Patel C, Lo Y, Oh M, Waickman A, Tam A, Blosser R, Wen J, Delgoffe G, Powell J. Asymmetric inheritance of mTORC1 kinase activity during division dictates CD8+ T cell differentiation. Nature Immunology 2016, 17: 704-711. PMID: 27064374, PMCID: PMC4873361, DOI: 10.1038/ni.3438.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell DifferentiationCell DivisionCell SurvivalCells, CulturedFemaleGlycolysisImmunologic MemoryLipid MetabolismLysosomesMaleMechanistic Target of Rapamycin Complex 1MiceMice, Inbred C57BLMice, TransgenicMultiprotein ComplexesPrecursor Cells, T-LymphoidProtein TransportReceptors, Antigen, T-CellSignal TransductionTOR Serine-Threonine Kinases
2014
The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex
Heikamp E, Patel C, Collins S, Waickman A, Oh M, Sun I, Illei P, Sharma A, Naray-Fejes-Toth A, Fejes-Toth G, Misra-Sen J, Horton M, Powell J. The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex. Nature Immunology 2014, 15: 457-464. PMID: 24705297, PMCID: PMC4267697, DOI: 10.1038/ni.2867.Peer-Reviewed Original ResearchMeSH KeywordsAdaptive ImmunityAnimalsAsthmaCell DifferentiationCells, CulturedEndosomal Sorting Complexes Required for TransportGene Expression RegulationHepatocyte Nuclear Factor 1-alphaImmediate-Early ProteinsInterferon-gammaMechanistic Target of Rapamycin Complex 2Melanoma, ExperimentalMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesNedd4 Ubiquitin Protein LigasesPoxviridae InfectionsProtein Serine-Threonine KinasesT Cell Transcription Factor 1Th1 CellsTh2 CellsTOR Serine-Threonine KinasesTranscription FactorsTumor BurdenUbiquitin-Protein LigasesVaccinia virus
2013
SHP-1 Regulation of Mast Cell Function in Allergic Inflammation and Anaphylaxis
Zhou L, Oh SY, Zhou Y, Yuan B, Wu F, Oh MH, Wang Y, Takemoto C, Van Rooijen N, Zheng T, Zhu Z. SHP-1 Regulation of Mast Cell Function in Allergic Inflammation and Anaphylaxis. PLOS ONE 2013, 8: e55763. PMID: 23390550, PMCID: PMC3563592, DOI: 10.1371/journal.pone.0055763.Peer-Reviewed Original ResearchMeSH KeywordsAdoptive TransferAllergensAnaphylaxisAnimalsBasophilsCell DifferentiationCells, CulturedCytokinesGene ExpressionInflammationInjections, IntravenousLipopolysaccharidesLungMast CellsMiceMice, KnockoutOvalbuminProtein Tyrosine Phosphatase, Non-Receptor Type 6Respiratory HypersensitivitySignal TransductionTh2 CellsConceptsTh2 immune responseAllergic inflammationAirway inflammationMast cell functionImmune responseMast cellsDeficient bone marrow cellsMast cell-deficient miceCell-deficient miceCell functionSevere allergic reactionsWild-type miceSHP-1Bone marrow cellsAirway stimulationAdoptive transferSystemic anaphylaxisAnaphylactic responseClinical symptomsImmunological homeostasisDeficient miceAllergic reactionsType miceAnaphylaxisInflammation
2009
SHP-1 Deficient Mast Cells Are Hyperresponsive to Stimulation and Critical in Initiating Allergic Inflammation in the Lung
Zhang L, Oh SY, Wu X, Oh MH, Wu F, Schroeder JT, Takemoto CM, Zheng T, Zhu Z. SHP-1 Deficient Mast Cells Are Hyperresponsive to Stimulation and Critical in Initiating Allergic Inflammation in the Lung. The Journal Of Immunology 2009, 184: 1180-1190. PMID: 20042576, PMCID: PMC4755278, DOI: 10.4049/jimmunol.0901972.Peer-Reviewed Original ResearchConceptsBone marrow-derived mast cellsAllergic asthma phenotypeMarrow-derived mast cellsMast cellsTh2 cytokinesMev miceAsthma phenotypesTh2 cytokines IL-4Allergic inflammatory responseTh2 cytokine productionRelease of mediatorsCytokines IL-4Mast cell productionT cells 2SHP-1 deficiencySrc homology region 2 domain-containing phosphatase-1Amount of mediatorMast cell developmentPulmonary inflammationAllergic inflammationCytokine productionTh2 phenotypeIL-13IL-4Deficient mast cells