2020
GABA interneurons are the cellular trigger for ketamine’s rapid antidepressant actions
Gerhard DM, Pothula S, Liu RJ, Wu M, Li XY, Girgenti MJ, Taylor SR, Duman CH, Delpire E, Picciotto M, Wohleb ES, Duman RS. GABA interneurons are the cellular trigger for ketamine’s rapid antidepressant actions. Journal Of Clinical Investigation 2020, 130: 1336-1349. PMID: 31743111, PMCID: PMC7269589, DOI: 10.1172/jci130808.Peer-Reviewed Original ResearchConceptsRapid antidepressant actionsAntidepressant actionGABA interneuronsMedial prefrontal cortexCell-specific knockdownPrinciple neuronsPrefrontal cortexDeletion of GluN2BSingle subanesthetic doseBehavioral actionsAction of ketamineNMDA receptor antagonistExcitatory postsynaptic currentsCellular triggersMajor unmet needKetamine's rapid antidepressant actionsGABA subtypeGluN2B-NMDARsSST interneuronsPostsynaptic currentsReceptor antagonistDepressed patientsSubanesthetic doseExtracellular glutamateMood disorders
2016
GABA interneurons mediate the rapid antidepressant-like effects of scopolamine
Wohleb ES, Wu M, Gerhard DM, Taylor SR, Picciotto MR, Alreja M, Duman RS. GABA interneurons mediate the rapid antidepressant-like effects of scopolamine. Journal Of Clinical Investigation 2016, 126: 2482-2494. PMID: 27270172, PMCID: PMC4922686, DOI: 10.1172/jci85033.Peer-Reviewed Original ResearchConceptsAntidepressant-like effectsMajor depressive disorderMedial prefrontal cortexRapid antidepressant-like effectsRapid antidepressant effectsM1-AChRAntidepressant effectsGABA interneuronsSST interneuronsM1-type muscarinic acetylcholine receptorsNonselective muscarinic acetylcholine receptor antagonistMuscarinic acetylcholine receptor antagonistAcetylcholine receptor antagonistMuscarinic acetylcholine receptorsViral-mediated knockdownPromising pharmacological targetActivity-dependent synapticAntidepressant therapyGABAergic neuronsSomatostatin interneuronsGlutamatergic neuronsSocioeconomic burdenGABAergic interneuronsGlutamatergic interneuronsReceptor antagonist
2000
Cholinergic Excitation of Septohippocampal GABA But Not Cholinergic Neurons: Implications for Learning and Memory
Wu M, Shanabrough M, Leranth C, Alreja M. Cholinergic Excitation of Septohippocampal GABA But Not Cholinergic Neurons: Implications for Learning and Memory. Journal Of Neuroscience 2000, 20: 3900-3908. PMID: 10804229, PMCID: PMC6772671, DOI: 10.1523/jneurosci.20-10-03900.2000.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAction PotentialsAnimalsAntibodies, MonoclonalCholinergic AgentsCognitionConditioning, PsychologicalElectrophysiologyGamma-Aminobutyric AcidHippocampusImmunotoxinsIn Vitro TechniquesMaleMemoryMuscarineMuscarinic AgonistsNeural InhibitionNeuronsN-Glycosyl HydrolasesRatsRats, Sprague-DawleyReceptor, Nerve Growth FactorReceptors, MuscarinicRibosome Inactivating Proteins, Type 1SaporinsSeptal NucleiSynaptic TransmissionTetrodotoxinTheta RhythmVasodilator AgentsConceptsMedial septum/diagonal bandSeptohippocampal cholinergic neuronsCholinergic neuronsCognition-enhancing effectsMuscarinic agonistsMuscarinic drugsContinuous theta rhythmHippocampal ACh releaseMuscarinic receptor antagonistMuscarinic cholinergic agonistRat brain slicesNovel fluorescent labelling techniqueDouble-labeling techniqueCholinergic excitationSeptohippocampal pathwayAcetylcholine releaseACh releaseDisinhibitory mechanismGABAergic pathwayReceptor antagonistUnlabeled neuronsCholinergic agonistsSystemic administrationDiagonal bandImpulse flow