2013
Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans
Collins KM, Koelle MR. Postsynaptic ERG Potassium Channels Limit Muscle Excitability to Allow Distinct Egg-Laying Behavior States in Caenorhabditis elegans. Journal Of Neuroscience 2013, 33: 761-775. PMID: 23303953, PMCID: PMC3542984, DOI: 10.1523/jneurosci.3896-12.2013.Peer-Reviewed Original ResearchConceptsEgg-laying musclesBody bendsUNC-103Caenorhabditis elegansCalcium transientsTwo-state behaviorERG potassium channelsMutantsERG channelsPotassium channelsInactive phaseEggsDistinct behavioral statesMuscle excitabilityPostsynaptic excitabilityPostsynaptic sitesERG functionCaenorhabditisCalcium imagingElegansFurther adjustmentExcitabilityRhythmic excitationMuscleBehavioral statesLIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans
Li P, Collins KM, Koelle MR, Shen K. LIN-12/Notch signaling instructs postsynaptic muscle arm development by regulating UNC-40/DCC and MADD-2 in Caenorhabditis elegans. ELife 2013, 2: e00378. PMID: 23539368, PMCID: PMC3601818, DOI: 10.7554/elife.00378.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCaenorhabditis elegansCaenorhabditis elegans ProteinsCalcium SignalingCell Adhesion MoleculesFemaleGenotypeIntracellular Signaling Peptides and ProteinsMorphogenesisMuscle ContractionMusclesMutationNeurogenesisOvipositionParacrine CommunicationPhenotypeReceptors, NotchSignal TransductionSodium ChannelsSynapsesVulvaConceptsPrecise synaptic connectivityNon-target musclesForm synapsesMADD-2Types of musclePresynaptic neuronsSynaptic targetsSynaptic connectivityCardinal featuresNervous systemGuidance moleculesTarget cellsLIN-12/NotchUNC-40/DCCMuscleCell typesArm extensionCellsMuscle armsEctopic expressionDiverse cell typesDCCArmExpressionNeurons
2009
The Potassium Chloride Cotransporter KCC-2 Coordinates Development of Inhibitory Neurotransmission and Synapse Structure in Caenorhabditis elegans
Tanis JE, Bellemer A, Moresco JJ, Forbush B, Koelle MR. The Potassium Chloride Cotransporter KCC-2 Coordinates Development of Inhibitory Neurotransmission and Synapse Structure in Caenorhabditis elegans. Journal Of Neuroscience 2009, 29: 9943-9954. PMID: 19675228, PMCID: PMC2737711, DOI: 10.1523/jneurosci.1989-09.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsChloridesFurosemideHypotonic SolutionsMotor NeuronsMusclesMutationReceptors, G-Protein-CoupledSequence HomologySexual Behavior, AnimalSodium Potassium Chloride Symporter InhibitorsSymportersSynapsesSynaptic TransmissionSynaptic VesiclesUp-RegulationConceptsEgg-laying behaviorChloride channelsC. elegans behaviorGenetic screenHSN neuronsMature neural circuitsChloride gradientFunctional analysisInhibitory neurotransmissionSynapse developmentVesicle populationsAdult mammalian brainSynaptic vesicle populationPotassium-chloride cotransporterTransport chlorideSynapse maturationPotassium-chloride cotransporter KCC2CaenorhabditisAppropriate activity levelsMammalian brainSynapse structureChloride cotransporterHypotonic conditionsLoop diuretic furosemideCoordinate development
2008
Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans
Tanis JE, Moresco JJ, Lindquist RA, Koelle MR. Regulation of Serotonin Biosynthesis by the G Proteins Gαo and Gαq Controls Serotonin Signaling in Caenorhabditis elegans. Genetics 2008, 178: 157-169. PMID: 18202365, PMCID: PMC2206068, DOI: 10.1534/genetics.107.079780.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCaenorhabditis elegansCaenorhabditis elegans ProteinsGene Expression Regulation, EnzymologicGTP-Binding Protein alpha Subunits, Gi-GoGTP-Binding Protein alpha Subunits, Gq-G11Motor NeuronsMusclesOrgan SpecificityOvipositionPromoter Regions, GeneticSerotoninSignal TransductionSynapsesTryptophan HydroxylaseConceptsEgg-laying behaviorCaenorhabditis elegansG proteinsEgg-laying systemHSN motor neuronsSerotonin biosynthesisG protein GαoLevel of transcriptionEpistasis experimentsTPH-1 expressionRate-limiting enzymeGene dosageSpecific genesGalphaoGalphaqTranscriptionBiosynthesisMotor neuronsElegansTPH-1Neurotransmitter releaseGenesMultiple signalsProteinEggs
2007
C. elegans G Protein Regulator RGS-3 Controls Sensitivity to Sensory Stimuli
Ferkey DM, Hyde R, Haspel G, Dionne HM, Hess HA, Suzuki H, Schafer WR, Koelle MR, Hart AC. C. elegans G Protein Regulator RGS-3 Controls Sensitivity to Sensory Stimuli. Neuron 2007, 53: 39-52. PMID: 17196529, PMCID: PMC1855255, DOI: 10.1016/j.neuron.2006.11.015.Peer-Reviewed Original ResearchConceptsSignal transductionG protein-coupled signal transductionRGS-3G protein signaling (RGS) proteinsHeterotrimeric G proteinsSpecific RGS proteinsRGS proteinsSensory neuronsSignaling proteinsMutant animalsNegative regulatorCalcium-binding proteinsG proteinsCalcium signalingBehavioral defectsTransductionSpecific odorantsProteinDefective responseRegulatorSensory behaviorsSynaptic transmissionIntense sensory stimuliExternal stimuliSignaling
2003
Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior
Bany IA, Dong MQ, Koelle MR. Genetic and Cellular Basis for Acetylcholine Inhibition of Caenorhabditis elegans Egg-Laying Behavior. Journal Of Neuroscience 2003, 23: 8060-8069. PMID: 12954868, PMCID: PMC6740490, DOI: 10.1523/jneurosci.23-22-08060.2003.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAcetylcholinesteraseAnimalsAnimals, Genetically ModifiedBehavior, AnimalCaenorhabditis elegansCaenorhabditis elegans ProteinsCholinesterase InhibitorsDNA, ComplementaryGTP-Binding ProteinsHomeodomain ProteinsInhibition, PsychologicalMutationNeuronsNuclear ProteinsOvipositionPhenotypeReceptors, CholinergicSignal TransductionSynapsesConceptsHermaphrodite-specific neuronsEgg-laying behaviorG proteinsG-protein signaling genesEgg-laying defectsEgg-laying musclesEgg-laying systemAnalysis of mutantsInhibition of eggSerotonergic Hermaphrodite Specific NeuronsUnc-4Caenorhabditis elegansUnc-17Signaling GenesThird cell typeActivation of eggsMorphological defectsCha-1MutantsCell typesCellular basisNeurotransmitter releaseGenesEggsPartial defect