Epithelial morphogenesis of MDCK cells in three-dimensional collagen culture is modulated by interleukin-8
Wells EK, Yarborough O, Lifton RP, Cantley LG, Caplan MJ. Epithelial morphogenesis of MDCK cells in three-dimensional collagen culture is modulated by interleukin-8. American Journal Of Physiology - Cell Physiology 2013, 304: c966-c975. PMID: 23485708, PMCID: PMC3651639, DOI: 10.1152/ajpcell.00261.2012.Peer-Reviewed Original ResearchConceptsMDCK cellsEpithelial morphogenesisHepatocyte growth factorGene expressionMDCK culturesDifferential gene expressionThree-dimensional collagen culturesReal-time PCR analysisGreater expression differencesMDCK cystsRenal epithelial cellsCollagen gelsGene setsTwo-dimensional cultureExpression differencesHGF stimulationThree-dimensional cultureMicroarray analysisSpherical cystsIL-8 protein expressionPCR analysisTubule-like structuresIL-8 participatesCollagen culturesProtein levels