2008
POSH decreases ROMK1 channel activity through stimulating clatharin‐independent and dynamin‐dependent endocytosis.
Lin D, Yue P, Sun P, Jin Y, Roos M, Caplan M, Wang W. POSH decreases ROMK1 channel activity through stimulating clatharin‐independent and dynamin‐dependent endocytosis. The FASEB Journal 2008, 22: 1180.1-1180.1. DOI: 10.1096/fasebj.22.1_supplement.1180.1.Peer-Reviewed Original Research
2007
Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels
Lin D, Kamsteeg R, Sterling H, Jin Y, Zhang Y, Roos M, Spencer J, Caplan M, Wang W. Expression of tetraspanin protein CD63 enhances the PTK‐induced inhibition of ROMK channels. The FASEB Journal 2007, 21: a1331-a1332. DOI: 10.1096/fasebj.21.6.a1331-d.Peer-Reviewed Original ResearchInhibition of PTKROMK channelsInhibitory effectK currentsHEK293 cellsC-SrcRenal outer medullaProtein tyrosine kinasesRole of CD63Tyrosine phosphorylationOuter medullaDecreased expressionTetraspanin protein CD63CD63Voltage clampElectrode voltage clampInhibitionHerbimycin AOocytesTyrosine kinaseXenopus oocytesPresent studyActivated PKCTetraspanin proteinsCoexpression
2000
Residues of the Fourth Transmembrane Segments of the Na,K-ATPase and the Gastric H,K-ATPase Contribute to Cation Selectivity*
Mense M, Dunbar L, Blostein R, Caplan M. Residues of the Fourth Transmembrane Segments of the Na,K-ATPase and the Gastric H,K-ATPase Contribute to Cation Selectivity*. Journal Of Biological Chemistry 2000, 275: 1749-1756. PMID: 10636871, DOI: 10.1074/jbc.275.3.1749.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAmino Acid SequenceAnimalsCationsElectrophysiologyH(+)-K(+)-Exchanging ATPaseHydrogen-Ion ConcentrationInhibitory Concentration 50KineticsMolecular Sequence DataMutationOuabainPotassiumRecombinant Fusion ProteinsSequence Homology, Amino AcidSodiumSodium-Potassium-Exchanging ATPaseStomachVanadatesXenopus laevisConceptsFourth transmembrane segmentTransmembrane segmentsATPase assaysK-ATPaseHelical wheel analysisTwo-electrode voltage-clamp experimentsCation selectivityProtein chimerasXenopus laevis oocytesVanadate sensitivityWild-type NaGastric HK-ATPasesXenopus oocytesLaevis oocytesATPase activityAbsence of sodiumResiduesTM4K counterpartsControl constructsOocytesConformational equilibriumAssaysImportant role