2022
The UIP/IPF fibroblastic focus is a collagen biosynthesis factory embedded in a distinct extracellular matrix
Herrera JA, Dingle L, Fernandez M, Venkateswaran RV, Blaikley JF, Lawless C, Schwartz MA. The UIP/IPF fibroblastic focus is a collagen biosynthesis factory embedded in a distinct extracellular matrix. JCI Insight 2022, 7: e156115. PMID: 35852874, PMCID: PMC9462507, DOI: 10.1172/jci.insight.156115.Peer-Reviewed Original Research
2016
Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling
Yun S, Budatha M, Dahlman JE, Coon BG, Cameron RT, Langer R, Anderson DG, Baillie G, Schwartz MA. Interaction between integrin α5 and PDE4D regulates endothelial inflammatory signalling. Nature Cell Biology 2016, 18: 1043-1053. PMID: 27595237, PMCID: PMC5301150, DOI: 10.1038/ncb3405.Peer-Reviewed Original ResearchConceptsInflammatory signalingIntegrin α5Enhanced phosphodiesterase activityExtracellular matrix remodellingModulates inflammationTherapeutic targetInflammationProstacyclin secretionLipid metabolismEndothelial cellsMatrix remodellingVivo knockdownECM remodellingBasement membraneIntegrin α2Phosphodiesterase activityMolecular mechanismsRemodellingΑ5Direct bindingSignalingCellsFibronectinAtherosclerosisArteryComparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effect
2011
JNK2 Promotes Endothelial Cell Alignment under Flow
Hahn C, Wang C, Orr AW, Coon BG, Schwartz MA. JNK2 Promotes Endothelial Cell Alignment under Flow. PLOS ONE 2011, 6: e24338. PMID: 21909388, PMCID: PMC3164210, DOI: 10.1371/journal.pone.0024338.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinase c-Jun N-terminal kinaseProtein kinase c-Jun N-terminal kinaseC-Jun N-terminal kinaseActin stress fibersN-terminal kinaseFocal adhesionsBasement membrane proteinsMembrane proteinsLaminar shear stressStress fibersGene expressionJNK activityIntegrin activationJNK2 activationEndothelial cell alignmentJNK activationActivated JNKExtracellular matrixInflammatory gene expressionCell alignmentUnexpected connectionEndothelial cellsActivationPathwayCells
2009
The Subendothelial Extracellular Matrix Modulates JNK Activation by Flow
Hahn C, Orr AW, Sanders JM, Jhaveri KA, Schwartz MA. The Subendothelial Extracellular Matrix Modulates JNK Activation by Flow. Circulation Research 2009, 104: 995-1003. PMID: 19286608, PMCID: PMC2702158, DOI: 10.1161/circresaha.108.186486.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApolipoproteins EAtherosclerosisBasement MembraneCattleCell Culture TechniquesCells, CulturedCollagenDisease Models, AnimalEndothelial CellsEnzyme ActivationExtracellular MatrixFibronectinsHemorheologyInflammationIntegrinsJNK Mitogen-Activated Protein KinasesMiceMice, Inbred C57BLMice, KnockoutMitogen-Activated Protein Kinase KinasesOscillometryP21-Activated KinasesPhosphorylationRegional Blood FlowStress, Mechanical
2005
The subendothelial extracellular matrix modulates NF-κB activation by flow
Orr AW, Sanders JM, Bevard M, Coleman E, Sarembock IJ, Schwartz MA. The subendothelial extracellular matrix modulates NF-κB activation by flow. Journal Of Cell Biology 2005, 169: 191-202. PMID: 15809308, PMCID: PMC2171897, DOI: 10.1083/jcb.200410073.Peer-Reviewed Original ResearchConceptsNF-kappaB activationSubendothelial extracellular matrixAtherosclerosis-prone sitesEarly monocyte recruitmentSigns of atherosclerosisFatty streak formationNovel therapeutic strategiesNF-κB activationSuppress NF-kappaB activationExtracellular matrixMonocyte recruitmentICAM-1VCAM-1Plaque formTherapeutic strategiesE-selectinP38-dependent pathwayNF-kappaBEndothelial cellsAtherosclerosisP38 activationNew integrinActivationStreak formationIntegrin alpha2beta1
2002
Effects of cell tension on the small GTPase Rac
Katsumi A, Milanini J, Kiosses WB, del Pozo MA, Kaunas R, Chien S, Hahn KM, Schwartz MA. Effects of cell tension on the small GTPase Rac. Journal Of Cell Biology 2002, 158: 153-164. PMID: 12105187, PMCID: PMC2173027, DOI: 10.1083/jcb.200201105.Peer-Reviewed Original ResearchMeSH KeywordsAmidesAnimalsAzepinesCell LineCell MembraneCell MovementCollagenDose-Response Relationship, DrugEnergy TransferGTP PhosphohydrolasesGuanine Nucleotide Exchange FactorsMicroscopy, FluorescenceMicroscopy, Phase-ContrastMicroscopy, VideoNaphthalenesNeoplasm ProteinsProteinsPseudopodiaPyridinesRac GTP-Binding ProteinsRatsStress, MechanicalTime FactorsT-Lymphoma Invasion and Metastasis-inducing Protein 1Transfection
2001
Ligation of Integrin α3β1 by Laminin 5 at the Wound Edge Activates Rho-dependent Adhesion of Leading Keratinocytes on Collagen*
Nguyen B, Ren X, Schwartz M, Carter W. Ligation of Integrin α3β1 by Laminin 5 at the Wound Edge Activates Rho-dependent Adhesion of Leading Keratinocytes on Collagen*. Journal Of Biological Chemistry 2001, 276: 43860-43870. PMID: 11571278, DOI: 10.1074/jbc.m103404200.Peer-Reviewed Original ResearchConceptsHuman foreskin keratinocytesLaminin-5Wound edgeCultured human foreskin keratinocytesRho-independent mechanismEndogenous laminin-5Collagen adhesionSpreading of keratinocytesTransition of keratinocytesForeskin keratinocytesDermal collagenKeratinocytesIntegrin α3β1Wound cellsActivation signalsQuiescent keratinocytesCollagenRac recruits high-affinity integrin αvβ3 to lamellipodia in endothelial cell migration
Kiosses W, Shattil S, Pampori N, Schwartz M. Rac recruits high-affinity integrin αvβ3 to lamellipodia in endothelial cell migration. Nature Cell Biology 2001, 3: 316-320. PMID: 11231584, DOI: 10.1038/35060120.Peer-Reviewed Original ResearchAndrostadienesAnimalsAntibodies, MonoclonalCattleCell MovementCells, CulturedChromonesCollagenEndothelium, VascularEnzyme InhibitorsGenes, ReporterImmunoglobulin FragmentsMicroinjectionsMicroscopy, FluorescenceMorpholinesPhosphoinositide-3 Kinase InhibitorsProtein BindingPseudopodiaRac GTP-Binding ProteinsReceptors, VitronectinRecombinant Fusion ProteinsTransfectionWortmanninIntegrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands
Jalali S, del Pozo M, Chen K, Miao H, Li Y, Schwartz M, Shyy J, Chien S. Integrin-mediated mechanotransduction requires its dynamic interaction with specific extracellular matrix (ECM) ligands. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 1042-1046. PMID: 11158591, PMCID: PMC14705, DOI: 10.1073/pnas.98.3.1042.Peer-Reviewed Original ResearchAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAntigens, CDCells, CulturedCollagenEndothelium, VascularExtracellular MatrixExtracellular Matrix ProteinsFibrinogenFibronectinsHumansIntegrin beta1Integrin beta3IntegrinsJNK Mitogen-Activated Protein KinasesLamininLigandsMitogen-Activated Protein KinasesPlatelet Membrane GlycoproteinsProteinsReceptors, VitronectinShc Signaling Adaptor ProteinsSignal TransductionSrc Homology 2 Domain-Containing, Transforming Protein 1Stress, MechanicalUmbilical VeinsVitronectin
1993
Integrin beta 1- and beta 3-mediated endothelial cell migration is triggered through distinct signaling mechanisms.
Leavesley D, Schwartz M, Rosenfeld M, Cheresh D. Integrin beta 1- and beta 3-mediated endothelial cell migration is triggered through distinct signaling mechanisms. Journal Of Cell Biology 1993, 121: 163-170. PMID: 7681432, PMCID: PMC2119781, DOI: 10.1083/jcb.121.1.163.Peer-Reviewed Original ResearchConceptsExtracellular calciumBeta 3Extracellular calcium sourcesAlpha v beta 3Alpha 2 beta 1Absence of cytokinesNa/H antiporterBeta 1 mAbIntracellular calciumCalcium influxIntegrin alpha 2 beta 1Specific influxBeta 1Distinct signaling mechanismsEndothelial cell migrationGrowth factorMeasurable riseDistinct intracellularRespective integrinsH antiporterCellular migrationCell migrationCell contactCalciumMAbs
1991
Multiple integrins share the ability to induce elevation of intracellular pH
Schwartz M, Ingber D, Lawrence M, Springer T, Lechene C. Multiple integrins share the ability to induce elevation of intracellular pH. Experimental Cell Research 1991, 195: 533-535. PMID: 1712734, DOI: 10.1016/0014-4827(91)90407-l.Peer-Reviewed Original ResearchConceptsBovine capillary endothelial cellsElevation of pHiAlpha v beta 3Alpha 5 beta 1Capillary endothelial cellsElevation of intracellularT cell receptor complexIntegrin alpha v beta 3Human T lymphocytesPeripheral human T lymphocytesNa-H antiporterCollagen type IIICell receptor complexIntegrin familyT cellsT lymphocytesSignificant elevationIntegrin LFA-1Integrin alpha 5 beta 1LFA-1ICAM-2Endothelial cellsBeta 1Beta 3Antibodies
1982
Analysis of platelet adhesion with a radioactive chemical crosslinking reagent: Interaction of thrombospondin with fibronectin and collagen
Lahav J, Schwartz M, Hynes R. Analysis of platelet adhesion with a radioactive chemical crosslinking reagent: Interaction of thrombospondin with fibronectin and collagen. Cell 1982, 31: 253-262. PMID: 7159924, DOI: 10.1016/0092-8674(82)90425-1.Peer-Reviewed Original Research