2019
The Type III Secretion System Sorting Platform
Lara-Tejero M. The Type III Secretion System Sorting Platform. Current Topics In Microbiology And Immunology 2019, 427: 133-142. PMID: 31183608, DOI: 10.1007/82_2019_167.Peer-Reviewed Original ResearchConceptsSecretion machineSecretion systemType III secretion injectisomeSecretion processType III secretion systemLarge cytoplasmic complexesProtein secretion machinesHost plasma membraneProtein translocasesCytoplasmic complexSecretion pathwayNeedle complexSorting platformPlasma membraneHost cellsProteinTranslocasesInjectisomeComplexesEffectorsPathwayComplex substructureSubstrateMembraneAssemblyThe Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells
Lara-Tejero M, Galán JE. The Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells. EcoSal Plus 2019, 8: 10.1128/ecosalplus.esp-0039-2018. PMID: 30942149, PMCID: PMC6450406, DOI: 10.1128/ecosalplus.esp-0039-2018.Peer-Reviewed Original ResearchConceptsEffector proteinsGram-negative bacteriaSecretion systemBacterial envelopeType III protein secretion systemProtein secretion systemHost cellular functionsEukaryotic plasma membraneEukaryotic cell membranesHost cell cytosolMultiprotein nanomachineEukaryotic hostsComplex nanomachinesImportant bacterial pathogensCellular functionsMammalian cellsNeedle complexOuter membranePlasma membraneCell cytosolInjectisomeCell membraneProteinBacterial pathogensCentral role
2017
In Situ Molecular Architecture of the Salmonella Type III Secretion Machine
Hu B, Lara-Tejero M, Kong Q, Galán JE, Liu J. In Situ Molecular Architecture of the Salmonella Type III Secretion Machine. Cell 2017, 168: 1065-1074.e10. PMID: 28283062, PMCID: PMC5393631, DOI: 10.1016/j.cell.2017.02.022.Peer-Reviewed Original ResearchConceptsType III secretion machinesSecretion machineNeedle complexType III protein secretion systemCytoplasmic sorting platformProtein secretion systemMulti-protein machinesProtein secretion machinesTarget eukaryotic cellsCryo-electron tomographyDifferent deletion mutantsSub-tomogram averagingSignificant conformational changesMolecular architectureExport apparatusEukaryotic cellsSecretion systemDeletion mutantsSorting platformConformational changesSitu structureMajor insightsMolecular modelingStructural componentsAssembly
2014
Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells
Galán JE, Lara-Tejero M, Marlovits TC, Wagner S. Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells. Annual Review Of Microbiology 2014, 68: 1-24. PMID: 25002086, PMCID: PMC4388319, DOI: 10.1146/annurev-micro-092412-155725.Peer-Reviewed Original ResearchConceptsEukaryotic cellsEffector proteinsBacterial envelopeType III secretion systemTarget eukaryotic cellsComplex nanomachinesNovel antimicrobial strategiesPathogen's benefitCellular functionsSecretion systemNeedle complexBacterial pathogenesisCytoplasmic componentsSpecialized nanomachinesSpecific substratesProteinPlantsCentral roleProtein deliveryAntimicrobial strategiesBacteriaNanomachinesTarget cellsCentral componentCells
2010
Organization and coordinated assembly of the type III secretion export apparatus
Wagner S, Königsmaier L, Lara-Tejero M, Lefebre M, Marlovits TC, Galán JE. Organization and coordinated assembly of the type III secretion export apparatus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 17745-17750. PMID: 20876096, PMCID: PMC2955140, DOI: 10.1073/pnas.1008053107.Peer-Reviewed Original ResearchConceptsExport apparatusNeedle complexType III protein secretion systemType III secretion machinesBacterial effector proteinsProtein secretion systemType III secretion export apparatusCryo-electron microscopyBacterial nanomachinesSecretion machineSymbiotic bacteriaEukaryotic cellsEffector proteinsSecretion systemMembrane proteinsApparatus componentsSubstantial coordinationBase assemblyProteinAssemblyEssential componentInsectsOrganellesComplexesBiology