2021
Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues
Kakade VR, Weiss M, Cantley LG. Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues. Frontiers In Physiology 2021, 12: 817181. PMID: 35002783, PMCID: PMC8727440, DOI: 10.3389/fphys.2021.817181.Peer-Reviewed Original ResearchSingle-cell sequencingComplex cellular microenvironmentHuman disease pathogenesisKidney cell typesCell identityHuman kidney samplesMultiplexed imaging techniquesMass cytometryCell sequencingCellular microenvironmentCell typesNovel insightsImaging Mass CytometryHuman tissuesDisease pathogenesisPathophysiological processesCellular dataAntibody validationProteomicsCytometryHuman kidneyIMC analysisSequencingKidney samplesPhenotyping
2019
Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry
Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight 2019, 4: e129477. PMID: 31217358, PMCID: PMC6629112, DOI: 10.1172/jci.insight.129477.Peer-Reviewed Original ResearchConceptsCell typesIndividual cell typesCritical baseline dataRenal cell typesMass cytometryQuantitative atlasNormal human samplesHuman kidneyRelative abundanceDevelopment of therapiesHuman kidney diseaseKidney diseaseMetal-conjugated antibodiesQuantitative interrogationScarce samplesMachine-learning pipelineDiscovery purposesFuture quantitative analysisNovel abnormalityNormal human kidneySingle tissue sectionHuman samplesRenal biopsyImmune cellsCells
2013
The Terminator mouse is a diphtheria toxin–receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages
Guo JK, Shi H, Koraishy F, Marlier A, Ding Z, Shan A, Cantley LG. The Terminator mouse is a diphtheria toxin–receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages. Kidney International 2013, 84: 1041-1046. PMID: 23739236, PMCID: PMC3775868, DOI: 10.1038/ki.2013.202.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBlotting, WesternCell LineageCell SeparationCell SurvivalDiphtheria ToxinGenotypeHeparin-binding EGF-like Growth FactorImmunohistochemistryIntegrasesIntercellular Signaling Peptides and ProteinsKidney Tubules, ProximalMiceMice, Inbred C57BLMice, TransgenicPhenotypePodocytesPrimary Cell CultureRNA, UntranslatedTime FactorsConceptsDiphtheria toxin treatmentToxin treatmentPrimary culturesDiphtheria toxin receptor expressionDiphtheria toxin receptorCell typesProximal tubule cellsPodocin-cre miceToxin exposureTubule cellsMiceMouse strainsWestern blottingToxin receptorKidney cellsSpecific cell typesMarker proteinsCell lineagesCellsTreatmentChapter 13 Principles of Cell Signaling
Cantley L. Chapter 13 Principles of Cell Signaling. 2013, 369-403. DOI: 10.1016/b978-0-12-381462-3.00013-6.Peer-Reviewed Original ResearchComplex multicellular organismsAppropriate cellular responsesCell-cell junctionsAppropriate cell surface receptorIntracellular second messengerCell surface receptorsMulticellular organismsNutrient acquisitionStructural organizerCell signalingLarger speciesDistant cellsCellular responsesSecond messengerRecipient cellsNumerous signalsCell typesCell surfaceIndividual cellsEffector moleculesSurface receptorsCoordinated seriesSingle cellsDiffusible factorsOrganisms