2016
High-resolution mass spectrometry of small molecules bound to membrane proteins
Gault J, Donlan JA, Liko I, Hopper JT, Gupta K, Housden NG, Struwe WB, Marty MT, Mize T, Bechara C, Zhu Y, Wu B, Kleanthous C, Belov M, Damoc E, Makarov A, Robinson CV. High-resolution mass spectrometry of small molecules bound to membrane proteins. Nature Methods 2016, 13: 333-336. PMID: 26901650, PMCID: PMC4856209, DOI: 10.1038/nmeth.3771.Peer-Reviewed Original Research
2013
Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins
Bhattacharyya M, Gupta K, Gowd KH, Balaram P. Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins. Molecular Omics 2013, 9: 1340-1350. PMID: 23467691, DOI: 10.1039/c3mb25534d.Peer-Reviewed Original ResearchConceptsMass spectrometric fragmentationDisulfide connectivityRapid mass spectrometric determinationPeptide toxinsNatural peptide toxinsMass spectrometric determinationCID fragmentationSpectrometric determinationNatural peptidesProteolytic nickProteolytic peptidesDirect fragmentationUnambiguous characterisationDisulfide linkagesSubsequent determinationSynthetic analoguesRapid screeningMS profilesDisulfide crosslinksStraightforward approachNative disulfidesDeterminationPeptidesFoldamersIntact polypeptide