2018
Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment
Bonello TT, Perez-Vale KZ, Sumigray KD, Peifer M. Rap1 acts via multiple mechanisms to position Canoe and adherens junctions and mediate apical-basal polarity establishment. Development 2018, 145: dev157941. PMID: 29361565, PMCID: PMC5825837, DOI: 10.1242/dev.157941.Peer-Reviewed Original ResearchMeSH KeywordsAdherens JunctionsAnimalsAnimals, Genetically ModifiedCell PolarityDrosophila melanogasterDrosophila ProteinsFemaleGastrulationGene Knockdown TechniquesGuanine Nucleotide Exchange FactorsIntracellular Signaling Peptides and ProteinsMaleModels, BiologicalProtein Interaction Domains and MotifsProtein TransportRNA InterferenceShelterin ComplexTelomere-Binding ProteinsConceptsApical-basal polarityPolarity establishmentMembrane localizationEpithelial apical-basal polarityAdherens junction positioningBazooka/Par3Adherens junction assemblyMost animal tissuesSmall GTPase Rap1Polarity initiationApical retentionNascent junctionsRA domainJunction assemblyAdherens junctionsTricellular junctionsPolarized cellsGTPase Rap1Macromolecular assembliesApical activationRap1Spatial organizationAnimal tissuesMultiple mechanismsEstablishment model
2011
Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis
Sumigray KD, Chen H, Lechler T. Lis1 is essential for cortical microtubule organization and desmosome stability in the epidermis. Journal Of Cell Biology 2011, 194: 631-642. PMID: 21844209, PMCID: PMC3160577, DOI: 10.1083/jcb.201104009.Peer-Reviewed Original ResearchMeSH Keywords1-Alkyl-2-acetylglycerophosphocholine EsteraseAlpha CateninAnimalsCarrier ProteinsCell DifferentiationCell ProliferationCells, CulturedDesmoplakinsDesmosomesEpidermisFluorescent Antibody TechniqueKeratinocytesMiceMice, KnockoutMicrotubule-Associated ProteinsMicrotubulesPermeabilityProtein TransportRecombinant Fusion ProteinsTransfectionConceptsDesmosomal protein desmoplakinCortical microtubule organizationCentrosomal proteinsMicrotubule organizationCell cortexMicrotubule reorganizationCell-cell adhesion structuresPenetrant perinatal lethalityDramatic defectsDesmosome stabilityCytoskeletal networkAdhesion structuresPerinatal lethalityUnexpected roleSingle isoformDesmosomal componentsBarrier activityCell typesDesmosomal proteinsEpidermal differentiationKeratin filamentsIntermediate filamentsProteinLIS1Specific subset