2019
Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut
Park J, Levic DS, Sumigray KD, Bagwell J, Eroglu O, Block CL, Eroglu C, Barry R, Lickwar CR, Rawls JF, Watts SA, Lechler T, Bagnat M. Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. Developmental Cell 2019, 51: 7-20.e6. PMID: 31474562, PMCID: PMC6783362, DOI: 10.1016/j.devcel.2019.08.001.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsApoptosis Regulatory ProteinsDietary ProteinsDisease Models, AnimalEnterocytesFemaleGastrointestinal MicrobiomeGene DeletionGene Expression Regulation, DevelopmentalIleumIntestinal AbsorptionIntestinesKwashiorkorLigandsLysosomesMaleMembrane ProteinsMiceReceptors, Cell SurfaceZebrafishZebrafish ProteinsConceptsFluid-phase endocytosisEndocytic machineryTrans-cellular transportLuminal protein digestionVertebrate gutLarval zebrafishCritical developmental stagesStomachless fishMolecular mechanismsVertebrate growthProtein uptakeDevelopmental stagesIntracellular digestionProtein digestionConditional deletionStunted growthIntestinal cellsOral acquisitionDab2Dietary proteinSevere protein malnutritionDigestive functionNeonatal mammalsProteinMalnutrition syndrome
2018
Spine Patterning Is Guided by Segmentation of the Notochord Sheath
Wopat S, Bagwell J, Sumigray KD, Dickson AL, Huitema LFA, Poss KD, Schulte-Merker S, Bagnat M. Spine Patterning Is Guided by Segmentation of the Notochord Sheath. Cell Reports 2018, 22: 2026-2038. PMID: 29466731, PMCID: PMC5860813, DOI: 10.1016/j.celrep.2018.01.084.Peer-Reviewed Original ResearchConceptsNotochord sheathTissue-specific manipulationActivation of NotchZebrafish notochordCentrum formationVertebral patterningOuter epithelial cell layerSheath cellsSegmentation defectsAxial structuresEpithelial cell layerNotochordPatterningIVD areaZebrafish spineDomainCell layerNotchCentraOsteoblastsSheathCellsActivation
2015
Epigenetic control of intestinal barrier function and inflammation in zebrafish
Marjoram L, Alvers A, Deerhake ME, Bagwell J, Mankiewicz J, Cocchiaro JL, Beerman RW, Willer J, Sumigray KD, Katsanis N, Tobin DM, Rawls JF, Goll MG, Bagnat M. Epigenetic control of intestinal barrier function and inflammation in zebrafish. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 2770-2775. PMID: 25730872, PMCID: PMC4352795, DOI: 10.1073/pnas.1424089112.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsUbiquitin-like proteinEpigenetic controlTumor necrosis factorEpigenetic repressionLoss of functionIntestinal epitheliumPrecise regulationInflammatory bowel diseasePromoter methylationBarrier functionChronic inflammationEpithelial cellsTNF inductionPotent cytokineImmune cell recruitmentIntestinal barrier functionCell sheddingTNFA expressionRing fingerProinflammatory stimuliProper responseExpressionHoming signalsCell recruitment