2017
High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches
Arasada R, Sayyad WA, Berro J, Pollard TD. High-speed super-resolution imaging of the proteins in fission yeast clathrin-mediated endocytic actin patches. Molecular Biology Of The Cell 2017, 29: mbc.e17-06-0415. PMID: 29212877, PMCID: PMC5996959, DOI: 10.1091/mbc.e17-06-0415.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonActin-Related Protein 2-3 ComplexActinsAdaptor Proteins, Vesicular TransportCell MembraneClathrinClathrin-Coated VesiclesCytokinesisCytoplasmEndocytosisMicrofilament ProteinsMicroscopy, FluorescenceMyosin Type IProtein BindingSchizosaccharomycesSchizosaccharomyces pombe ProteinsConceptsActin filamentsLive fission yeast cellsEndocytic actin patchesSites of endocytosisFission yeast cellsNucleation-promoting factorsActin binding proteinsActin filament polymerizationCell surface receptorsActin patchesVesicle scissionFission yeastYeast clathrinArp2/3 complexSuperresolution localization microscopyTubule elongationFilament polymerizationRegulated mannerRegulatory proteinsMolecular mechanismsYeast cellsBinding proteinClathrinIndependent pathwaysCell surface
2014
Synergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast
Berro J, Pollard TD. Synergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast. Molecular Biology Of The Cell 2014, 25: 3515-3527. PMID: 25143407, PMCID: PMC4230613, DOI: 10.1091/mbc.e13-01-0005.Peer-Reviewed Original ResearchConceptsActin-depolymerizing factorActin filamentsFission yeastNew cellular functionsHeterodimeric capping proteinClathrin-mediated endocytosisActin filament barbedEndocytic patchesEndocytic sitesCellular functionsAip1pCapping proteinEndocytic vesiclesFilament barbedCapping activityPlasma membraneProtein subunitsActin meshworkCell polarizationIndependent pathwaysProteinEndocytosisYeastQuantitative microscopyFilamentsLocal and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method
Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method. Molecular Biology Of The Cell 2014, 25: 3501-3514. PMID: 25143395, PMCID: PMC4230612, DOI: 10.1091/mbc.e13-01-0004.Peer-Reviewed Original ResearchConceptsFission yeastEndocytic actin patchesWild-type cellsEndocytic patchesActin patchesQuantitative microscopyActin assemblyCellular processesVesicle movementEndocytic vesiclesInterphase cellsVesicle formationMolecular mechanismsPatch dynamicsYeastCell lengthGlobal analysisNumber of patchesMicroscopy moviesCellsClathrinEndocytosisNew toolValuable toolPatches
2010
Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast
Sirotkin V, Berro J, Macmillan K, Zhao L, Pollard TD. Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast. Molecular Biology Of The Cell 2010, 21: 2894-2904. PMID: 20587778, PMCID: PMC2921122, DOI: 10.1091/mbc.e10-02-0157.Peer-Reviewed Original ResearchConceptsFission yeastActin assemblyActin patch assemblyEndocytic actin patchesEndocytic adaptor proteinActivators of Arp2/3Plasma membrane invaginationsRecruitment of dynaminQuantitative confocal microscopyActin filament fragmentationActin patchesArp2/3 complexPatch assemblyAdaptor proteinMembrane invaginationsCross-linked filamentsFluorescent proteinClathrin moleculesFilament fragmentationProteinConfocal microscopyYeastBranched networkIndividual patchesAssembly