2021
PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation
Li D, Taylor D, van Wolfswinkel J. PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation. Cell Reports 2021, 37: 109776. PMID: 34610311, PMCID: PMC8532177, DOI: 10.1016/j.celrep.2021.109776.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsCell differentiationDefective cell differentiationLineage-specific genesSubset of transposonsAdult pluripotent stem cellsSomatic cell differentiationStem cell systemStem cell biologyPluripotent stem cellsTransposon derepressionAnimal germlineSpecific chromatinRegenerative animalsPlanarian neoblastsCell biologyTransposonStem cellsProteinDifferentiationIntricate interplayCell systemAddition causesTissue dysfunction
2014
Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment
van Wolfswinkel JC, Wagner DE, Reddien PW. Single-Cell Analysis Reveals Functionally Distinct Classes within the Planarian Stem Cell Compartment. Cell Stem Cell 2014, 15: 326-339. PMID: 25017721, PMCID: PMC4171737, DOI: 10.1016/j.stem.2014.06.007.Peer-Reviewed Original ResearchConceptsSingle-cell transcriptional profilingDistinct cellular compartmentsGene expression fingerprintPluripotent stem cellsStem cell compartmentProliferative cell populationDistinct classesTranscriptional profilingCellular compartmentsPlanarian neoblastsExpression fingerprintsBroad lineagesEpidermal cellsNeoblastsStem cellsCell compartmentLineagesCell populationsCell analysisCellsCompartmentsPlanariansFlatwormsRegenerationHomeostasisWhole-Body Acoel Regeneration Is Controlled by Wnt and Bmp-Admp Signaling
Srivastava M, Mazza-Curll K, van Wolfswinkel J, Reddien P. Whole-Body Acoel Regeneration Is Controlled by Wnt and Bmp-Admp Signaling. Current Biology 2014, 24: 1107-1113. PMID: 24768051, DOI: 10.1016/j.cub.2014.03.042.Peer-Reviewed Original ResearchConceptsWhole-body regenerationMolecular mechanismsLast common ancestorEarly branching positionDorsal-ventral axisYears of evolutionEnigmatic phylumBilaterian evolutionMolecular resourcesUnderlying molecular mechanismsSystemic RNAiAccessible embryosCommon ancestorHofsteniaPhylogenetic analysisVentral cellsAcoelsMolecular studiesTranscriptomePhylaRNAiPlanariansAncestorBranching positionModel systemMUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation
Phillips C, Montgomery B, Breen P, Roovers E, Rim Y, Ohsumi T, Newman M, van Wolfswinkel J, Ketting R, Ruvkun G, Montgomery T. MUT-14 and SMUT-1 DEAD Box RNA Helicases Have Overlapping Roles in Germline RNAi and Endogenous siRNA Formation. Current Biology 2014, 24: 839-844. PMID: 24684932, PMCID: PMC4010136, DOI: 10.1016/j.cub.2014.02.060.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCaenorhabditis elegansCaenorhabditis elegans ProteinsDEAD-box RNA HelicasesFluoroimmunoassayGerm CellsImmunoprecipitationMolecular Sequence DataReal-Time Polymerase Chain ReactionRNA InterferenceRNA, Small InterferingSaccharomyces cerevisiaeSequence AlignmentSequence Analysis, DNAConceptsSiRNA formationMutator fociClass genesDEAD-box RNA helicasesBox RNA helicasesElegans genesEndogenous siRNAsSiRNA amplificationMutant displaysP granulesNuclear peripheryRNA helicasesMissense mutantsMutant proteinsDouble mutantMUT-16Target mRNAsRNAiGerm cellsGenesEarly stepsGermline genesSiRNAsMutantsWidespread loss
2012
Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
Kamminga LM, van Wolfswinkel JC, Luteijn MJ, Kaaij LJ, Bagijn MP, Sapetschnig A, Miska EA, Berezikov E, Ketting RF. Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans. PLOS Genetics 2012, 8: e1002702. PMID: 22829772, PMCID: PMC3400576, DOI: 10.1371/journal.pgen.1002702.Peer-Reviewed Original ResearchConceptsHENN-1G RNAAGO proteinsSRNA moleculesSmall RNAsGene expressionC. elegans piRNAsGermline-expressed genesSequence-specific recruitmentDifferent RNAi pathwaysGlobal gene expression analysisGlobal gene expressionLoss of methylationGene expression analysisEndogenous RNAiRNAi pathwayC. elegansRNA classesRNA displayGene activityRNA interferenceExpression analysisSequence specificityTarget RNARNA
2009
CDE-1 Affects Chromosome Segregation through Uridylation of CSR-1-Bound siRNAs
van Wolfswinkel JC, Claycomb JM, Batista PJ, Mello CC, Berezikov E, Ketting RF. CDE-1 Affects Chromosome Segregation through Uridylation of CSR-1-Bound siRNAs. Cell 2009, 139: 135-148. PMID: 19804759, DOI: 10.1016/j.cell.2009.09.012.Peer-Reviewed Original ResearchConceptsCDE-1Chromosome segregationCSR-1RNAi pathwaySimilar sorting mechanismsMitotic chromosome segregationEndogenous RNAi pathwaysEGO-1Argonaute proteinsMitotic chromosomesSiRNA levelsC. elegansConserved natureSorting mechanismSiRNAsUridylationChromosomesInappropriate loadingProteinPathwaySegregationElegansRNAiMammalsGenes
2005
Chromatin and RNAi factors protect the C. elegans germline against repetitive sequences
Robert VJ, Sijen T, van Wolfswinkel J, Plasterk RH. Chromatin and RNAi factors protect the C. elegans germline against repetitive sequences. Genes & Development 2005, 19: 782-787. PMID: 15774721, PMCID: PMC1074315, DOI: 10.1101/gad.332305.Peer-Reviewed Original ResearchConceptsRepetitive sequencesRepetitive transgenesCaenorhabditis elegans germlineC. elegans germlineProtection of genomeRNA interference screenChromatin factorsRNAi factorsTranscriptional geneChromatin remodelingEndogenous genesInterference screenMolecular dataCatalog genesSelective silencingPutative roleGenesRNAiGermlineSequenceTransgeneTrans effectCosuppressionChromatinGenome