2000
Large-Scale Culture and Selective Maturation of Human Langerhans Cells from Granulocyte Colony-Stimulating Factor-Mobilized CD34+ Progenitors
Gatti E, Velleca M, Biedermann B, Ma W, Unternaehrer J, Ebersold M, Medzhitov R, Pober J, Mellman I. Large-Scale Culture and Selective Maturation of Human Langerhans Cells from Granulocyte Colony-Stimulating Factor-Mobilized CD34+ Progenitors. The Journal Of Immunology 2000, 164: 3600-3607. PMID: 10725716, DOI: 10.4049/jimmunol.164.7.3600.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, CD1Antigens, CD34Antigens, Differentiation, T-LymphocyteAntigens, NeoplasmCD40 LigandCell CountCell Culture TechniquesCell DifferentiationDendritic CellsGranulocyte Colony-Stimulating FactorHematopoietic Stem Cell TransplantationHumansImmunophenotypingLangerhans CellsLeukapheresisLigandsLipopolysaccharidesMembrane GlycoproteinsStem CellsTumor Necrosis Factor-alphaConceptsDendritic cellsLangerhans cellsT cell stimulationG-CSF-mobilized patientsManipulation of DCsImmature dendritic cellsIL-12 productionPrimary immune responseHuman Langerhans cellsCell stimulationDifferent proinflammatory stimuliToll family receptorsDC preparationsMaturation stimuliTNF-alphaCD40 ligandMHC productsImmune responseBirbeck granulesProinflammatory stimuliCytokine removalFamily receptorsAg processingDC typesHomogenous population
1997
Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis
Mach F, Schönbeck U, Sukhova G, Bourcier T, Bonnefoy J, Pober J, Libby P. Functional CD40 ligand is expressed on human vascular endothelial cells, smooth muscle cells, and macrophages: Implications for CD40–CD40 ligand signaling in atherosclerosis. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 1931-1936. PMID: 9050882, PMCID: PMC20020, DOI: 10.1073/pnas.94.5.1931.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteArteriosclerosisBlotting, WesternB-LymphocytesCD40 AntigensCD40 LigandCells, CulturedEndothelium, VascularFlow CytometryGene Expression RegulationHumansImmunohistochemistryInterferon-gammaInterleukin-1MacrophagesMembrane GlycoproteinsMuscle, Smooth, VascularRNA, MessengerSignal TransductionTumor Necrosis Factor-alphaConceptsHuman vascular endothelial cellsSmooth muscle cellsVascular endothelial cellsHuman atherosclerotic lesionsHuman macrophagesCell typesEndothelial cellsMuscle cellsHuman vascular smooth muscle cellsVascular smooth muscle cellsDe novo synthesisCD40 ligandBroad functionsAtherosclerotic lesionsCD40 SignalingTumor necrosis factor alphaFunctional CD40 ligandInvolvement of inflammationCultured human vascular endothelial cellsCD40-CD40 ligandNovo synthesisNecrosis factor alphaParacrine activationNormal arterial tissueNovel source
1996
Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway
Karmann K, Hughes C, Fanslow W, Pober J. Endothelial cells augment the expression of CD40 ligand on newly activated human CD4+ T cells through a CD2/LFA‐3 signaling pathway. European Journal Of Immunology 1996, 26: 610-617. PMID: 8605928, DOI: 10.1002/eji.1830260316.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntigens, Differentiation, T-LymphocyteB-LymphocytesCD2 AntigensCD40 LigandCD4-Positive T-LymphocytesCD58 AntigensCell AdhesionCell CommunicationCells, CulturedEndothelium, VascularHumansInterleukin-2LigandsLymphocyte ActivationMembrane GlycoproteinsPhytohemagglutininsSignal TransductionUp-RegulationConceptsCD40 ligand expressionExogenous IL-2T cellsIL-2Ligand expressionCD2/LFACD40 ligandHuman peripheral blood CD4Peripheral blood adherent cellsHelper T cell subsetsPeripheral blood T cellsPeripheral blood CD4T cell subsetsCo-stimulatory moleculesBlood T cellsHelper T cellsIL-2 receptorCo-stimulatory signalsIL-2 secretionInterleukin-2 secretionBlood CD4Endothelial effectsCell subsetsHuman CD4B cells
1993
HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM
SAVAGE C, HUGHES C, MCINTYRE B, PICARD J, POBER J. HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM. Transplantation 1993, 56: 128-134. PMID: 7687392, DOI: 10.1097/00007890-199307000-00024.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, T-LymphocyteCD2 AntigensCD4 AntigensCD58 AntigensCells, CulturedEndothelium, VascularFlow CytometryHistocompatibility Antigens Class IHLA-DR AntigensHumansInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsPolymerase Chain ReactionReceptors, ImmunologicRecombinant ProteinsT-Lymphocyte SubsetsTransplantation, HomologousUmbilical VeinsConceptsHLA-DR expressing cellsT cellsHLA-DRPolymerase chain reactionEC culturesAllogeneic class II MHC moleculesMHC moleculesLFA-1 beta chainLFA-1Peripheral blood CD4VLA-4 alphaMHC class II moleculesT cell responsesClass II MHC moleculesT cell surface moleculesT cell proliferationClass I MHC moleculesExpressing cellsClass II moleculesBeta chainII MHC moleculesDe novo expressionI MHC moleculesAllogeneic proliferationBlood CD4
1991
Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells
Savage C, Hughes C, Pepinsky R, Wallner B, Freedman A, Pober J. Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells. Cellular Immunology 1991, 137: 150-163. PMID: 1679377, DOI: 10.1016/0008-8749(91)90065-j.Peer-Reviewed Original ResearchConceptsLymphocyte function-associated antigen-3Adhesion molecule-1T cellsEndothelial cellsMolecule-1Antigen 3Human peripheral blood CD4Vascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Peripheral blood CD4T cell populationsB7/BB1Cultured human endothelial cellsIL-2 synthesisBlood CD4EC costimulationHuman endothelial cellsT cell CD2IL-2CD4CostimulationCell populationsComparable degreeLines of evidenceINDUCED EXPRESSION OF ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULES IN HUMAN CARDIAC ALLOGRAFTS
Briscoe DM, Schoen FJ, Rice GE, Bevilacqua MP, Ganz P, Pober JS. INDUCED EXPRESSION OF ENDOTHELIAL-LEUKOCYTE ADHESION MOLECULES IN HUMAN CARDIAC ALLOGRAFTS. Transplantation 1991, 51: 537-539. PMID: 1704649, DOI: 10.1097/00007890-199102000-00050.Peer-Reviewed Original Research
1990
The Endothelial Cell as a Regulator of T‐Cell Function
Hughes. C, Savage C, Pober JS. The Endothelial Cell as a Regulator of T‐Cell Function. Immunological Reviews 1990, 117: 85-102. PMID: 2258192, DOI: 10.1111/j.1600-065x.1990.tb00568.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Presenting CellsAntigens, Differentiation, T-LymphocyteEndothelium, VascularHumansMajor Histocompatibility ComplexReceptors, ImmunologicT-LymphocytesConceptsIL-2 productionT cell IL-2 productionT cellsClass II moleculesLFA-3Peripheral blood mononuclear cellsAntigen-presenting capacityMemory T cellsMHC class II moleculesBlood mononuclear cellsNon-immune cell typesT cell functionNon-polymorphic regionsT cell linesCostimulatory cellsMononuclear cellsIL-2Allospecific CTLICAM-1IFN-gammaVCAM-1Immunosuppressive drug CsAAbility of ECCostimulator activityVLA-4Lymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression.
Doukas J, Pober JS. Lymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression. The Journal Of Immunology 1990, 145: 1088-98. PMID: 1974272, DOI: 10.4049/jimmunol.145.4.1088.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteCD4 AntigensCD4-Positive T-LymphocytesCD8 AntigensCell Adhesion MoleculesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansImmune SeraInterferon-gammaPhytohemagglutininsT-Lymphocytes, RegulatoryConceptsClass II MHC expressionIntercellular adhesion molecule-1 expressionClass I MHCAdhesion molecule-1 expressionClass II AgClass II expressionMolecule-1 expressionT cellsMHC expressionIFN-gammaI MHCEndothelial cellsICAM-1Class II MHC inductionClass IClass II MHC AgCoculture of CD4Failure of CD4Immunoreactive IFN-gammaICAM-1 expressionClass II MHCCultured endothelial cellsUnfractionated PBLTime of inductionMHC inductionEndothelial cells augment T cell interleukin 2 production by a contact-dependent mechanism involving CD2/LFA-3 interaction.
Hughes CC, Savage CO, Pober JS. Endothelial cells augment T cell interleukin 2 production by a contact-dependent mechanism involving CD2/LFA-3 interaction. Journal Of Experimental Medicine 1990, 171: 1453-1467. PMID: 1692079, PMCID: PMC2187887, DOI: 10.1084/jem.171.5.1453.Peer-Reviewed Original Research
1988
Effects of recombinant gamma-interferon on HLA-DR and DQ expression by skin cells in short-term organ culture.
Messadi DV, Pober JS, Murphy GF. Effects of recombinant gamma-interferon on HLA-DR and DQ expression by skin cells in short-term organ culture. Laboratory Investigation 1988, 58: 61-7. PMID: 3121914.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteCells, CulturedDendritic CellsEndothelium, VascularEpidermisHLA-D AntigensHLA-DQ AntigensHLA-DR AntigensHumansInterferon-gammaOrgan Culture TechniquesRecombinant ProteinsSkinConceptsShort-term organ cultureHLA-DRLangerhans cellsOrgan cultureT6-positive Langerhans cellsClass II major histocompatibility antigensImmune interferonClass II major histocompatibility moleculesEpidermal dendritic cellsCutaneous immune responsesDendritic cell populationsHLA-DR expressionSkin microvascular endothelial cellsMajor histocompatibility antigensMicrovascular endothelial cellsSkin cellsNewborn human foreskinMajor histocompatibility moleculesDendritic cellsInflammatory mediatorsDQ expressionHLA-DQOrgan culture systemHistocompatibility antigensImmune response