2001
Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes
Dengler T, Johnson D, Pober J. Human Vascular Endothelial Cells Stimulate a Lower Frequency of Alloreactive CD8+ Pre-CTL and Induce Less Clonal Expansion than Matching B Lymphoblastoid Cells: Development of a Novel Limiting Dilution Analysis Method Based on CFSE Labeling of Lymphocytes. The Journal Of Immunology 2001, 166: 3846-3854. PMID: 11238628, DOI: 10.4049/jimmunol.166.6.3846.Peer-Reviewed Original ResearchAntigens, CDB-Lymphocyte SubsetsCD8-Positive T-LymphocytesCell DivisionCell Line, TransformedCells, CulturedClone CellsCoculture TechniquesColony-Forming Units AssayCytotoxicity Tests, ImmunologicEndothelium, VascularFlow CytometryFluoresceinsFluorescent DyesHumansImmunologic MemoryInterphaseIsoantigensLymphocyte ActivationLymphocyte CountStem CellsSuccinimidesT-Lymphocyte SubsetsT-Lymphocytes, Regulatory
2000
Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype
Dengler T, Pober J. Human Vascular Endothelial Cells Stimulate Memory But Not Naive CD8+ T Cells to Differentiate into CTL Retaining an Early Activation Phenotype. The Journal Of Immunology 2000, 164: 5146-5155. PMID: 10799873, DOI: 10.4049/jimmunol.164.10.5146.Peer-Reviewed Original ResearchMeSH KeywordsB-LymphocytesCD8-Positive T-LymphocytesCell DifferentiationCells, CulturedCoculture TechniquesCyclosporineCytotoxicity Tests, ImmunologicEndothelium, VascularHistocompatibility Antigens Class IHumansImmunologic MemoryImmunophenotypingInterleukin-12InterphaseLeukocyte Common AntigensLymphocyte ActivationT-Lymphocyte SubsetsT-Lymphocytes, CytotoxicConceptsB lymphoblastoid cellsT cellsNaive CD8Endothelial cellsImmunoregulatory cell typesIntracellular perforin contentAlloreactive T cellsAnti-CD28 mAbHuman vascular endothelial cellsHigh surface expressionVascular endothelial cellsExpansion of memoryConventional CTLGraft parenchymaGraft rejectionMemory CD8CTL generationPerforin contentCTL expansionVascular injuryHuman CD8CD40 ligandAlloreactive CTLAnatomic compartmentsICAM-1