2009
TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart
Al-Lamki R, Brookes AP, Wang J, Reid MJ, Parameshwar J, Goddard MJ, Tellides G, Wan T, Min W, Pober JS, Bradley JR. TNF Receptors Differentially Signal and Are Differentially Expressed and Regulated in the Human Heart. American Journal Of Transplantation 2009, 9: 2679-2696. PMID: 19788501, PMCID: PMC3517885, DOI: 10.1111/j.1600-6143.2009.02831.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell CycleCell DeathEndothelium, VascularEnzyme ActivationGraft RejectionHeart TransplantationHumansMAP Kinase Kinase Kinase 5MiceMice, KnockoutMyocardiumMyocytes, CardiacOrgan Culture TechniquesProtein-Tyrosine KinasesReceptors, Tumor Necrosis Factor, Type IReceptors, Tumor Necrosis Factor, Type IIRNA, MessengerTumor Necrosis Factor-alphaConceptsVascular endothelial cellsCardiac allograftsCell cycle entryApoptosis signal-regulating kinase 1Cycle entryExpression of TNFTNFR1 knockoutNecrosis factorTarget cell responseTNFTNF receptorCardiac fibroblastsCell responsesSignal-regulated kinases 1TNF responseASK1 activationMyocardiumEndothelial cellsEpithelial tyrosine kinaseTNFR2Human heartOrgan cultureTNFR1Etk activationAllografts
2007
Tumor Necrosis Factor
Pober J. Tumor Necrosis Factor. 2007, 261-265. DOI: 10.1017/cbo9780511546198.032.Peer-Reviewed Original ResearchHemorrhagic necrosisNecrosis factorMediator of cachexiaMediators of inflammationTumor necrosis factorPrimary cellular sourceAdaptive immune systemTNF-like moleculeTime of cloningMajor histocompatibility complexPeyer's patchesEndogenous mediatorsT lymphocytesMast cellsParasitic infectionsTNFBacterial productsExperimental tumorsImmune systemMononuclear phagocytesCellular sourceSmall intestineHistocompatibility complexTNF geneRelated receptors
2006
Endothelial Cell Dysfunction, Injury and Death
Pober JS, Min W. Endothelial Cell Dysfunction, Injury and Death. Handbook Of Experimental Pharmacology 2006, 176/II: 135-156. PMID: 16999227, DOI: 10.1007/3-540-36028-x_5.Peer-Reviewed Original ResearchConceptsTumor necrosis factorCytolytic T lymphocytesEC injuryEndothelial cellsReactive oxygen speciesEndothelial cell dysfunctionMacrophage-derived cytokinesVascular endothelial cellsEndothelial dysfunctionNecrosis factorCell dysfunctionT lymphocytesInjuryCommon mediatorInflammationDysfunctionDeathOxygen speciesCytokinesLymphocytesNeutrophilsDifferent agentsBiochemical pathways
2001
HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1
Tereb D, Kirkiles-Smith N, Kim R, Wang Y, Rudic R, Schechner J, Lorber M, Bothwell A, Pober J, Tellides G. HUMAN T CELLS INFILTRATE AND INJURE PIG CORONARY ARTERY GRAFTS WITH ACTIVATED BUT NOT QUIESCENT ENDOTHELIUM IN IMMUNODEFICIENT MOUSE HOSTS1. Transplantation 2001, 71: 1622-1630. PMID: 11435975, DOI: 10.1097/00007890-200106150-00023.Peer-Reviewed Original ResearchConceptsTumor necrosis factorHuman tumor necrosis factorArtery graftT cellsNecrosis factorPig coronary artery endothelial cellsHuman T-cell infiltrationVascular cell adhesion molecule-1Human peripheral blood mononuclearHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsHuman leukocytesEndothelial cellsMajor histocompatibility complex antigensCell adhesion molecule-1Graft endothelial cellsT-cell infiltratesBeige mouse modelCoronary artery endothelial cellsT cell infiltrationCoronary artery graftsBlood mononuclear cellsPeripheral blood mononuclearCoronary artery segmentsAdhesion molecule-1TNF Signaling in Vascular Endothelial Cells
Madge L, Pober J. TNF Signaling in Vascular Endothelial Cells. Experimental And Molecular Pathology 2001, 70: 317-325. PMID: 11418010, DOI: 10.1006/exmp.2001.2368.Peer-Reviewed Original ResearchConceptsTumor necrosis factorEndothelial cellsProinflammatory cytokine tumor necrosis factorCytokines tumor necrosis factorCultured human endothelial cellsVascular endothelial cellsHuman endothelial cellsNecrosis factorVascular endotheliumIntracellular pathwaysMajor targetTNF signalingCell typesCellsEndothelial cell activation by tumor necrosis factor elicits human antiporcine cell-mediated rejection responses
Kirkiles-Smith NC, Tereb DA, Kim RW, McNiff JM, Schechner JS, Lorber MI, Pober JS, Tellides G. Endothelial cell activation by tumor necrosis factor elicits human antiporcine cell-mediated rejection responses. Transplantation Proceedings 2001, 33: 412-413. PMID: 11266888, DOI: 10.1016/s0041-1345(00)02072-8.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsCytokinesEndothelium, VascularGraft RejectionHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansLymphocyte ActivationMiceMice, SCIDModels, AnimalSkin TransplantationSpecies SpecificitySwineT-LymphocytesTransplantation ChimeraTransplantation ToleranceTransplantation, HeterologousTumor Necrosis Factor-alphaConceptsTumor necrosis factorEndothelial cell activationNecrosis factorCell activationRejection response
1997
Evidence that tumor necrosis factor triggers apoptosis in human endothelial cells by interleukin-1-converting enzyme-like protease-dependent and -independent pathways.
Slowik MR, Min W, Ardito T, Karsan A, Kashgarian M, Pober JS. Evidence that tumor necrosis factor triggers apoptosis in human endothelial cells by interleukin-1-converting enzyme-like protease-dependent and -independent pathways. Laboratory Investigation 1997, 77: 257-67. PMID: 9314949.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsBcl-xLEndothelial cellsEnzyme-like proteasesType 1 TNF receptorBcl-2Antiapoptotic protein Bcl-2Protein synthesis inhibitor cycloheximideProtein Bcl-2CrmA proteinTumor necrosis factorCombination of TNFCultured human endothelial cellsZ-VADTriggers apoptosisSeparable pathwaysIndependent pathwaysTNF muteinsInhibitor cycloheximideProtein A20Necrosis factorEC injuryTNFApoptosisTNF receptor
1996
Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells
Slowik M, De Luca L, Min W, Pober J. Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells. Circulation Research 1996, 79: 736-747. PMID: 8831497, DOI: 10.1161/01.res.79.4.736.Peer-Reviewed Original ResearchConceptsConcentrations of TNFNF-kappa BEndothelial cellsJun N-terminal kinaseNecrosis factorEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Treatment of ECCell deathCultured human umbilical vein endothelial cellsEndothelial leukocyte adhesion moleculeTumor necrosis factorAdhesion molecule-1Leukocyte adhesion moleculesHuman vascular endothelial cellsHuman umbilical vein endothelial cellsStimulation of ECUmbilical vein endothelial cellsVascular endothelial cellsP50/p65 heterodimeric formVein endothelial cellsPotential intracellular mediatorsActivation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1.
Karmann K, Min W, Fanslow WC, Pober JS. Activation and homologous desensitization of human endothelial cells by CD40 ligand, tumor necrosis factor, and interleukin 1. Journal Of Experimental Medicine 1996, 184: 173-182. PMID: 8691131, PMCID: PMC2192678, DOI: 10.1084/jem.184.1.173.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCalcium-Calmodulin-Dependent Protein KinasesCD40 LigandCell Adhesion MoleculesEndothelium, VascularE-SelectinGene ExpressionHumansIntercellular Adhesion Molecule-1Interleukin-1JNK Mitogen-Activated Protein KinasesMembrane GlycoproteinsMitogen-Activated Protein KinasesMolecular Sequence DataOligodeoxyribonucleotidesRNA, MessengerTranscription, GeneticTumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1ConceptsVascular cell adhesion molecule-1Intercellular adhesion molecule-1Tumor necrosis factorHuman umbilical vein endothelial cellsAdhesion molecule-1CD40 ligandHomologous desensitizationIL-1E-selectinJun NH2-terminal kinaseNecrosis factorInterleukin-1Molecule-1ATF-2/c-JunEndothelial cellsCell adhesion molecule-1
1995
CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS. CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4342-4346. PMID: 7538666, PMCID: PMC41940, DOI: 10.1073/pnas.92.10.4342.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, B-LymphocyteCD40 AntigensCell Adhesion MoleculesCells, CulturedCytokinesEndothelium, VascularGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHumansInterferon-betaInterferon-gammaInterleukinsKineticsMiceRecombinant ProteinsSkinTumor Necrosis Factor-alphaUmbilical VeinsConceptsTumor necrosis factorAdhesion molecule-1Endothelial cellsHuman endothelial cellsNecrosis factorInterleukin-1Molecule-1T cell-mediated inflammatory reactionVascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Cultured human umbilical vein endothelial cellsAdhesion molecule expressionLeukocyte adhesion moleculesMicrovascular endothelial cellsHuman microvascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsFlow cytometric quantitationVein endothelial cellsCD40 antigenEndothelial CD40Interferon betaMolecule expressionInflammatory reaction
1994
Inducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients
Petzelbauer P, Pober J, Keh A, Braverman I. Inducibility and Expression of Microvascular Endothelial Adhesion Molecules in Lesional, Perilesional, and Uninvolved Skin of Psoriatic Patients. Journal Of Investigative Dermatology 1994, 103: 300-305. PMID: 7521374, DOI: 10.1111/1523-1747.ep12394720.Peer-Reviewed Original ResearchConceptsVascular cell adhesion molecule-1Endothelial leukocyte adhesion molecule-1Lesional skinAdhesion molecule-1Psoriatic patientsEndothelial cellsUninvolved skinPerilesional skinMolecule-1Dermal microvasculatureCapillary loopsCell adhesion molecule-1Perivascular inflammatory infiltratesLeukocyte adhesion molecule-1VCAM-1 expressionTumor necrosis factorEndothelial adhesion moleculesMicrovascular endothelial cellsEndothelial cell expressionVenular endothelial cellsInflammatory infiltrateEpidermal involvementPsoriatic plaquesSuperficial plexusNecrosis factor
1993
Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration.
Slowik MR, De Luca LG, Fiers W, Pober JS. Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration. American Journal Of Pathology 1993, 143: 1724-30. PMID: 7504889, PMCID: PMC1887273.Peer-Reviewed Original ResearchConceptsEndothelial leukocyte adhesion molecule-1Endothelial cellsHuman endothelial cellsTumor necrosis factor concentrationsMonoclonal antibodiesNecrosis factor concentrationsLeukocyte adhesion molecule-1Major histocompatibility complex moleculesActivation of ECTumor necrosis factorAdhesion molecule-1Class I major histocompatibility complex moleculesLeukocyte adhesion moleculesTumor necrosis factor receptorELAM-1 inductionRecombinant human TNFHistocompatibility complex moleculesNecrosis factor receptorCultured endothelial cellsP75 receptorTumor necrosisNecrosis factorMolecule-1Receptor typesTNF receptor
1991
Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon.
Redl H, Dinges HP, Buurman WA, van der Linden CJ, Pober JS, Cotran RS, Schlag G. Expression of endothelial leukocyte adhesion molecule-1 in septic but not traumatic/hypovolemic shock in the baboon. American Journal Of Pathology 1991, 139: 461-6. PMID: 1714243, PMCID: PMC1886066.Peer-Reviewed Original ResearchConceptsEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Hypovolemic shockSeptic shockAdhesion molecule-1Endothelial leukocyte adhesion molecule-1 expressionMolecule-1Adhesion molecule-1 expressionGranulocyte elastase levelsMolecule-1 expressionELAM-1 expressionTumor necrosis factorDe novo expressionEndothelial activationElastase levelsLive Escherichia coliNeutrophil activationNecrosis factorImmunohistochemical techniquesELAM-1ELAM expressionStrong stainingNovo expressionSmall veinsHigh levelsInterleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells.
Rollins BJ, Pober JS. Interleukin-4 induces the synthesis and secretion of MCP-1/JE by human endothelial cells. American Journal Of Pathology 1991, 138: 1315-9. PMID: 2053591, PMCID: PMC1886397.Peer-Reviewed Original ResearchConceptsMCP-1/JEIL-4Interleukin-1Endothelial cellsHuman endothelial cellsJE proteinInterleukin 4 inducesTreatment of ECMCP-1/JE mRNATumor necrosis factorMonocyte-specific chemoattractantAnti-tumor propertiesConcomitant treatmentNecrosis factorInterleukin-4SecretionMore secretionJE mRNAInduced expressionTreatment
1990
Cytokine-endothelial interactions in inflammation, immunity, and vascular injury.
Cotran RS, Pober JS. Cytokine-endothelial interactions in inflammation, immunity, and vascular injury. Journal Of The American Society Of Nephrology 1990, 1: 225-35. PMID: 2104268, DOI: 10.1681/asn.v13225.Peer-Reviewed Original ResearchConceptsVascular injuryTumor necrosis factorEffects of cytokinesEndothelial adhesion moleculesLeukocyte-endothelial adhesionCytokine-induced activationSuch pathological processesSeptic shockNecrosis factorImmune responseInterleukin-1Shwartzman reactionAdhesion moleculesPathological processesCytokinesInjuryStructural alterationsBiological characteristicsPossible contributionVasculitisInflammationEndotheliumHypersensitivityImmunityImportant roleTumor necrosis factor and immune interferon synergistically increase transcription of HLA class I heavy- and light-chain genes in vascular endothelium.
Johnson DR, Pober JS. Tumor necrosis factor and immune interferon synergistically increase transcription of HLA class I heavy- and light-chain genes in vascular endothelium. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 5183-5187. PMID: 2164225, PMCID: PMC54286, DOI: 10.1073/pnas.87.13.5183.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCell NucleusCells, CulturedDrug SynergismEndothelium, VascularFlow CytometryGenes, MHC Class IHistocompatibility Antigens Class IHumansInterferon Type IInterferon-gammaMacromolecular SubstancesMolecular Sequence DataReceptors, Cell SurfaceReceptors, Tumor Necrosis FactorRecombinant ProteinsRNA, MessengerSequence Homology, Nucleic AcidTranscription, GeneticTumor Necrosis Factor-alphaConceptsNecrosis factorHuman endothelial cellsEndothelial cellsClass IInterferon gammaImmune interferonMRNA levelsMajor histocompatibility complex moleculesTumor necrosis factorHLA class IClass I major histocompatibility complex moleculesCultured human endothelial cellsHistocompatibility complex moleculesUntreated endothelial cellsSynergistic increaseCytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE.
Rollins BJ, Yoshimura T, Leonard EJ, Pober JS. Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. American Journal Of Pathology 1990, 136: 1229-33. PMID: 2113354, PMCID: PMC1877579.Peer-Reviewed Original ResearchConceptsMCP-1/JEIL-1 betaEndothelial cellsMonocyte chemoattractantMonocyte chemoattractant MCP-1Tumor necrosis factorChemoattractant MCP-1Accumulation of monocytesSites of inflammationMonocyte chemoattractant activityHours of treatmentHuman endothelial cellsInflammatory mediatorsMCP-1Necrosis factorIFN-gammaChemoattractant activityJE proteinChemoattractantCellsBetaHoursInducible expressionMRNALesser extent
1989
Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis.
Munro JM, Pober JS, Cotran RS. Tumor necrosis factor and interferon-gamma induce distinct patterns of endothelial activation and associated leukocyte accumulation in skin of Papio anubis. American Journal Of Pathology 1989, 135: 121-33. PMID: 2505619, PMCID: PMC1880213.Peer-Reviewed Original ResearchConceptsLeukocyte accumulationNecrosis factorIFN-gammaEndothelial cell antigen expressionAdhesion moleculesAnti-intercellular adhesion moleculeEndothelial cell hypertrophyPolymorphonuclear leukocyte accumulationTumor necrosis factorCytokine-activated endotheliumLeukocyte adhesion moleculesCell antigen expressionImmune inflammationEndothelial activationLeukocyte infiltrationHypersensitivity reactionsMononuclear cellsHLA-DPEnvelope antigenAntigen expressionVascular permeabilitySubsequent extravasationRecombinant human interferonCell hypertrophyEndothelial morphology
1988
Three distinct classes of regulatory cytokines control endothelial cell MHC antigen expression. Interactions with immune gamma interferon differentiate the effects of tumor necrosis factor and lymphotoxin from those of leukocyte alpha and fibroblast beta interferons.
Lapierre LA, Fiers W, Pober JS. Three distinct classes of regulatory cytokines control endothelial cell MHC antigen expression. Interactions with immune gamma interferon differentiate the effects of tumor necrosis factor and lymphotoxin from those of leukocyte alpha and fibroblast beta interferons. Journal Of Experimental Medicine 1988, 167: 794-804. PMID: 2450953, PMCID: PMC2188900, DOI: 10.1084/jem.167.3.794.Peer-Reviewed Original ResearchConceptsIFN-alphaIL-6Endothelial cellsClass IAction of lymphotoxinMHC antigen expressionIFN-alpha/betaClass II antigensClass I expressionClass II inductionTumor necrosis factorCultured human endothelial cellsClass I MHCRecombinant preparationsHuman endothelial cellsCytokine administrationMHC antigensII antigensPatient populationRegulatory cytokinesNecrosis factorAntigen expressionGamma interferonIFN-gammaI MHC
1987
Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture.
Messadi DV, Pober JS, Fiers W, Gimbrone MA, Murphy GF. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture. The Journal Of Immunology 1987, 139: 1557-62. PMID: 3497975, DOI: 10.4049/jimmunol.139.5.1557.Peer-Reviewed Original ResearchConceptsTumor necrosis factorActivation antigensNecrosis factorInterleukin-1Microvascular endotheliumEndothelial cellsEndothelial activation antigenMediators interleukin-1Postcapillary venular endotheliumCultured human umbilical vein endothelial cellsSuperficial vascular plexusOrgan cultureHuman skin organ cultureShort-term organ cultureHuman umbilical vein endothelial cellsSkin organ cultureUmbilical vein endothelial cellsVenular endothelial cellsMicrovascular stainingPostcapillary venular endothelial cellsVein endothelial cellsEndogenous cytokinesLymphocyte traffickingInterleukin-2Venular endothelium