2022
Monobody adapter for functional antibody display on nanoparticles for adaptable targeted delivery applications
Albert C, Bracaglia L, Koide A, DiRito J, Lysyy T, Harkins L, Edwards C, Richfield O, Grundler J, Zhou K, Denbaum E, Ketavarapu G, Hattori T, Perincheri S, Langford J, Feizi A, Haakinson D, Hosgood SA, Nicholson ML, Pober JS, Saltzman WM, Koide S, Tietjen GT. Monobody adapter for functional antibody display on nanoparticles for adaptable targeted delivery applications. Nature Communications 2022, 13: 5998. PMID: 36220817, PMCID: PMC9553936, DOI: 10.1038/s41467-022-33490-8.Peer-Reviewed Original ResearchConceptsTargeted delivery applicationsTargeted nanoparticlesAntibody immobilizationAntigen-binding functionNanoparticlesNP surfaceDelivery applicationsRobust deliveryEndothelial cellsAmine couplingAntibody displayClinical translationIntracellular drug levelsVascular endothelial cellsCultured endothelial cellsNanomedicineDrug levelsVivo perfusionOrgan transplantsAdapterClinical settingHuman kidneyHuman settingImmobilizationEfficacy
2013
Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation
Devalliere J, Chang WG, Andrejecsk JW, Abrahimi P, Cheng CJ, Jane‐wit D, Saltzman WM, Pober JS. Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation. The FASEB Journal 2013, 28: 908-922. PMID: 24221087, PMCID: PMC3898640, DOI: 10.1096/fj.13-238527.Peer-Reviewed Original ResearchConceptsHuman umbilical vein ECsEndothelial cellsMiR-132MicroRNA-132Cultured human umbilical vein endothelial cellsNumber of microvesselsGrowth factor-induced proliferationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationCultured endothelial cellsEndogenous growth factorsEC transplantationVein endothelial cellsCell transplantationImmunodeficient miceTissue perfusionTransplantationMiR deliveryGrowth factorIntegrin αvβ3Endocytosed nanoparticlesSquare millimeterBiological effectsControl transfection
1993
Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration.
Slowik MR, De Luca LG, Fiers W, Pober JS. Tumor necrosis factor activates human endothelial cells through the p55 tumor necrosis factor receptor but the p75 receptor contributes to activation at low tumor necrosis factor concentration. American Journal Of Pathology 1993, 143: 1724-30. PMID: 7504889, PMCID: PMC1887273.Peer-Reviewed Original ResearchConceptsEndothelial leukocyte adhesion molecule-1Endothelial cellsHuman endothelial cellsTumor necrosis factor concentrationsMonoclonal antibodiesNecrosis factor concentrationsLeukocyte adhesion molecule-1Major histocompatibility complex moleculesActivation of ECTumor necrosis factorAdhesion molecule-1Class I major histocompatibility complex moleculesLeukocyte adhesion moleculesTumor necrosis factor receptorELAM-1 inductionRecombinant human TNFHistocompatibility complex moleculesNecrosis factor receptorCultured endothelial cellsP75 receptorTumor necrosisNecrosis factorMolecule-1Receptor typesTNF receptor
1992
Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells.
Birch KA, Pober JS, Zavoico GB, Means AR, Ewenstein BM. Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells. Journal Of Cell Biology 1992, 118: 1501-1510. PMID: 1522120, PMCID: PMC2289613, DOI: 10.1083/jcb.118.6.1501.Peer-Reviewed Original ResearchConceptsVon Willebrand factorEndothelial cellsProtein kinase CCell-permeant calcium chelatorExtracellular calcium chelator EGTAVWF secretionAgonist-induced elevationInhibitory peptidesSignal transduction pathwaysHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsCultured endothelial cellsCalcium chelator EGTAPermeabilized human umbilical vein endothelial cellsVein endothelial cellsHuman endothelial cellsPhorbol esterRole of calmodulinSecond messenger pathwaysIntracellular calciumIntact human endothelial cellsTransduction pathwaysThrombin receptorCalcium ionophoreLarge intracellular protein
1990
IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1.
Doukas J, Pober JS. IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1. The Journal Of Immunology 1990, 145: 1727-33. PMID: 1697308, DOI: 10.4049/jimmunol.145.6.1727.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernCell Adhesion MoleculesDrug SynergismE-SelectinEndothelium, VascularFluorescent Antibody TechniqueGene ExpressionHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-1Recombinant ProteinsRNA, MessengerTumor Necrosis Factor-alphaConceptsEndothelial leukocyte adhesion molecule-1ELAM-1 expressionIL-1 betaIFN-gammaIL-1Endothelial cellsEC activationIL-1 beta responseIntercellular adhesion molecule-1 expressionAdhesion molecule-1 expressionMHC Ag expressionLeukocyte adhesion molecule-1Treatment of ECICAM-1 expressionMolecule-1 expressionClass I MHC moleculesTumor necrosis factorAdhesion molecule-1ICAM-1 mRNAClass I MHCI MHC moleculesIFN-gamma effectELAM-1 mRNACultured endothelial cellsIncrease TNFLymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression.
Doukas J, Pober JS. Lymphocyte-mediated activation of cultured endothelial cells (EC). CD4+ T cells inhibit EC class II MHC expression despite secreting IFN-gamma and increasing EC class I MHC and intercellular adhesion molecule-1 expression. The Journal Of Immunology 1990, 145: 1088-98. PMID: 1974272, DOI: 10.4049/jimmunol.145.4.1088.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, Differentiation, T-LymphocyteCD4 AntigensCD4-Positive T-LymphocytesCD8 AntigensCell Adhesion MoleculesCells, CulturedEndothelium, VascularHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHumansImmune SeraInterferon-gammaPhytohemagglutininsT-Lymphocytes, RegulatoryConceptsClass II MHC expressionIntercellular adhesion molecule-1 expressionClass I MHCAdhesion molecule-1 expressionClass II AgClass II expressionMolecule-1 expressionT cellsMHC expressionIFN-gammaI MHCEndothelial cellsICAM-1Class II MHC inductionClass IClass II MHC AgCoculture of CD4Failure of CD4Immunoreactive IFN-gammaICAM-1 expressionClass II MHCCultured endothelial cellsUnfractionated PBLTime of inductionMHC induction
1988
Endothelial activation during interleukin 2 immunotherapy. A possible mechanism for the vascular leak syndrome.
Cotran RS, Pober JS, Gimbrone MA, Springer TA, Wiebke EA, Gaspari AA, Rosenberg SA, Lotze MT. Endothelial activation during interleukin 2 immunotherapy. A possible mechanism for the vascular leak syndrome. The Journal Of Immunology 1988, 140: 1883-8. PMID: 3279124, DOI: 10.4049/jimmunol.140.6.1883.Peer-Reviewed Original ResearchConceptsIntercellular adhesion molecule-1Adhesion molecule-1Endothelial cell activationVascular leak syndromeEndothelial leukocyte adhesion molecule-1Leak syndromeMolecule-1IL-2Cell activationCultured endothelial cellsEndothelial expressionImmune responseEndothelial leukocyte adhesion molecule-1 expressionLeu-10Cell-mediated immune responsesAdhesion molecule-1 expressionEndothelial cellsDifferent activation antigensInterleukin-2 immunotherapyPost-treatment skinIL-2 therapyPostcapillary venular endotheliumMolecule-1 expressionIL-2 actsDays of treatment
1987
Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells.
Ewenstein BM, Warhol MJ, Handin RI, Pober JS. Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells. Journal Of Cell Biology 1987, 104: 1423-1433. PMID: 3494734, PMCID: PMC2114468, DOI: 10.1083/jcb.104.5.1423.Peer-Reviewed Original ResearchConceptsWeibel-Palade bodiesSecretory organellesUmbilical vein endothelial cellsOrganelle fractionsVein endothelial cellsCultured endothelial cellsIntracellular protein traffickingVon Willebrand factorVon Willebrand factor storageEndothelial cellsSelf-generating Percoll gradientsDirect morphological examinationCultured umbilical vein endothelial cellsUnmodified formProtein traffickingCultured human umbilical vein endothelial cellsRegulated secretionStimulation of ECHuman umbilical vein endothelial cellsStorage organellesOrganellesDense organellesPro sequenceProteolytic processingAdhesive glycoprotein