Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH
Savulescu A, Brackin R, Bouilhol E, Dartigues B, Warrell J, Pimentel M, Beaume N, Fortunato I, Dallongeville S, Boulle M, Soueidan H, Agou F, Schmoranzer J, Olivo-Marin J, Franco C, Gomes E, Nikolski M, Mhlanga M. Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH. Cell Reports Methods 2021, 1: 100068. PMID: 35474672, PMCID: PMC9017151, DOI: 10.1016/j.crmeth.2021.100068.Peer-Reviewed Original ResearchConceptsSubcellular localizationSingle-molecule RNA fluorescenceSingle-cell RNA sequencingNumber of transcriptsSmFISH dataRNA fluorescenceRNA sequencingHybridization dataBiological insightsLocalization patternsSubcellular distributionPatterns of clusteringCellular architectureRNACellular subtypesSkeletal muscle fibersCell micropatterningProtein immunolabelingPhysiological systemsCellsLocalizationBasis of quantificationSpatial distributionTranscriptsSequencing