1990
A fusion-defective mutant of the vesicular stomatitis virus glycoprotein
Whitt M, Zagouras P, Crise B, Rose J. A fusion-defective mutant of the vesicular stomatitis virus glycoprotein. Journal Of Virology 1990, 64: 4907-4913. PMID: 2168975, PMCID: PMC247981, DOI: 10.1128/jvi.64.10.4907-4913.1990.Peer-Reviewed Original ResearchConceptsWild-type G proteinG proteinsMutant proteinsFusion activityMutant G proteinsFusion-defective mutantsAmino acids 117Vesicular stomatitis virus glycoproteinFormation of heterotrimersUncharged amino acidsTemperature-sensitive mutantNew glycosylation siteMutant glycoproteinsVesicular stomatitis virusGlycosylation sitesMembrane fusionRescue of virusVSV virionsExtracellular domainAmino acidsCell surfaceProteinVSV serotypesStomatitis virusMutants
1985
Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein.
Adams G, Rose J. Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein. Molecular And Cellular Biology 1985, 5: 1442-1448. PMID: 2993864, PMCID: PMC366875, DOI: 10.1128/mcb.5.6.1442.Peer-Reviewed Original ResearchConceptsMembrane anchoringG proteinsAmino acidsCell surfaceIsoleucine residueMembrane-spanning domainsCell surface transportVesicular stomatitis virus glycoproteinOligonucleotide-directed mutagenesisAmino acid sequenceUncharged amino acidsDetectable protein levelsHydrophobic amino acidsAnimal cellsCDNA clonesIntracellular membranesAcid sequencePunctate patternGolgi regionProteinContinuous stretchVesicular patternProtein levelsViral glycoproteinsVirus glycoproteinIncorporation of a Charged Amino Acid into the Membrane-Spanning Domain Blocks Cell Surface Transport But Not Membrane Anchoring of a Viral Glycoprotein
Adams G, Rose J. Incorporation of a Charged Amino Acid into the Membrane-Spanning Domain Blocks Cell Surface Transport But Not Membrane Anchoring of a Viral Glycoprotein. Molecular And Cellular Biology 1985, 5: 1442-1448. DOI: 10.1128/mcb.5.6.1442-1448.1985.Peer-Reviewed Original ResearchMembrane anchoringG proteinsAmino acidsCell surfaceIsoleucine residueMembrane-spanning domainsCell surface transportVesicular stomatitis virus glycoproteinOligonucleotide-directed mutagenesisAmino acid sequenceUncharged amino acidsDetectable protein levelsHydrophobic amino acidsAnimal cellsCDNA clonesIntracellular membranesAcid sequencePunctate patternGolgi regionProteinContinuous stretchVesicular patternProtein levelsViral glycoproteinsVirus glycoprotein