2019
Effects of Endotoxin on Type 3 Inositol 1,4,5‐Trisphosphate Receptor in Human Cholangiocytes
Franca A, Filho A, Guerra MT, Weerachayaphorn J, dos Santos M, Njei B, Robert M, Lima C, Vidigal P, Banales JM, Ananthanarayanan M, Leite MF, Nathanson MH. Effects of Endotoxin on Type 3 Inositol 1,4,5‐Trisphosphate Receptor in Human Cholangiocytes. Hepatology 2019, 69: 817-830. PMID: 30141207, PMCID: PMC6351171, DOI: 10.1002/hep.30228.Peer-Reviewed Original ResearchConceptsToll-like receptor 4Alcoholic hepatitisEffect of endotoxinBile duct cellsNF-κBInhibition of TLR4Human cholangiocytesStimulation of TLR4Duct cellsSevere alcoholic hepatitisCholestasis of sepsisForms of cholestasisNF-κB subunitsP65/p50Trisphosphate receptorReceptor 4Clinical conditionsBicarbonate secretionHepatocellular changesITPR3 expressionCholestasisType 3 inositolLPS receptorAgonist stimulusSepsis
2015
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes
Weerachayaphorn J, Amaya MJ, Spirli C, Chansela P, Mitchell-Richards KA, Ananthanarayanan M, Nathanson MH. Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes. Gastroenterology 2015, 149: 211-222.e10. PMID: 25796361, PMCID: PMC4478166, DOI: 10.1053/j.gastro.2015.03.014.Peer-Reviewed Original ResearchConceptsBile ductBile duct unitsCholestatic disordersOxidative stressCalcium signalingNuclear factorMouse cholangiocytesDuct unitsReduced calcium signalingIntrahepatic bile ductsLevels of Nrf2Cholangiocyte cellsKnockdown of Nrf2Activation of Nrf2Intracellular calcium release channelsTranscription factor Nrf2Binding of Nrf2Calcium release channelPolymerase chain reaction analysisBiliary diseaseTrisphosphate receptorControl ratsLiver disordersBicarbonate secretionChain reaction analysis
2007
Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2
Weerachayaphorn J, Pajor AM. Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2. Biochimica Et Biophysica Acta 2007, 1778: 1051-1059. PMID: 18194662, PMCID: PMC2323910, DOI: 10.1016/j.bbamem.2007.12.013.Peer-Reviewed Original ResearchBiological TransportCaco-2 CellsCitratesCitric Acid CycleColonic NeoplasmsDicarboxylic Acid TransportersEpithelial CellsExtracellular SpaceGene Expression RegulationHumansIntracellular SpaceKineticsModels, BiologicalOrganic Anion TransportersOrganic Anion Transporters, Sodium-DependentPlasticsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodiumSubstrate SpecificitySuccinatesSymportersTime FactorsTransfectionSodium-dependent Extracellular Accessibility of Lys-84 in the Sodium/Dicarboxylate Cotransporter*
Weerachayaphorn J, Pajor AM. Sodium-dependent Extracellular Accessibility of Lys-84 in the Sodium/Dicarboxylate Cotransporter*. Journal Of Biological Chemistry 2007, 282: 20213-20220. PMID: 17504760, PMCID: PMC2864014, DOI: 10.1074/jbc.m701113200.Peer-Reviewed Original ResearchConceptsLys-84Transmembrane helicesMTS inhibitionLarge-scale conformational changesTranslocation of substratesCysteine-specific reagentsTransmembrane helix 3SLC13 familyCitric acid cycle intermediatesExtracellular halfEpithelial cell lineExtracellular accessibilityHelix 3Transport cycleTrp-103Human retinal pigment epithelial cell lineAddition of substrateConformational changesLeu-111Conformational statesRetinal pigment epithelial cell lineSubstrate affinityReentrant loopAmino acidsGlu-101