2015
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes
Weerachayaphorn J, Amaya MJ, Spirli C, Chansela P, Mitchell-Richards KA, Ananthanarayanan M, Nathanson MH. Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes. Gastroenterology 2015, 149: 211-222.e10. PMID: 25796361, PMCID: PMC4478166, DOI: 10.1053/j.gastro.2015.03.014.Peer-Reviewed Original ResearchConceptsBile ductBile duct unitsCholestatic disordersOxidative stressCalcium signalingNuclear factorMouse cholangiocytesDuct unitsReduced calcium signalingIntrahepatic bile ductsLevels of Nrf2Cholangiocyte cellsKnockdown of Nrf2Activation of Nrf2Intracellular calcium release channelsTranscription factor Nrf2Binding of Nrf2Calcium release channelPolymerase chain reaction analysisBiliary diseaseTrisphosphate receptorControl ratsLiver disordersBicarbonate secretionChain reaction analysis
2007
Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †
Weerachayaphorn J, Pajor AM. Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †. Biochemistry 2007, 47: 1087-1093. PMID: 18161988, PMCID: PMC2570185, DOI: 10.1021/bi701417h.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionAnimalsChlorocebus aethiopsCitric AcidCOS CellsDicarboxylic Acid TransportersGene ExpressionHumansKineticsMolecular Sequence DataMutationOrganic Anion Transporters, Sodium-DependentRabbitsRecombinant ProteinsSequence Homology, Amino AcidSodiumSubstrate SpecificitySuccinic AcidSymportersThreonineConceptsTransmembrane helix 7Functional differencesApparent affinityApparent sodium affinitySubstrate Binding SiteAmino acid differencesCitric acid cycleCitrate affinityHelix 7Substrate specificityPosition -509Rabbit sequenceIndividual residuesAcid cycleAcid differencesSimilar apparent affinitiesLoop regionIntermediate KmAmino acidsBinding sitesMutantsNaDC1SerineThreonineExhibit differences