2017
Type 2 inositol trisphosphate receptor gene expression in hepatocytes is regulated by cyclic AMP
Kruglov E, Ananthanarayanan M, Sousa P, Weerachayaphorn J, Guerra MT, Nathanson MH. Type 2 inositol trisphosphate receptor gene expression in hepatocytes is regulated by cyclic AMP. Biochemical And Biophysical Research Communications 2017, 486: 659-664. PMID: 28327356, PMCID: PMC5421629, DOI: 10.1016/j.bbrc.2017.03.086.Peer-Reviewed Original ResearchMeSH KeywordsAdenylyl CyclasesAnimalsBinding SitesColforsinCREB-Binding ProteinCyclic AMPDactinomycinFastingGene Expression RegulationHep G2 CellsHepatocytesHumansInositol 1,4,5-Trisphosphate ReceptorsMaleMutationPrimary Cell CulturePromoter Regions, GeneticProtein BindingRatsRats, Sprague-DawleyResponse ElementsRNA, MessengerSignal TransductionThionucleotidesConceptsPost-translational modificationsRecruitment of CREBAdenylyl cyclase 6Transcriptional regulationType 2 inositolGene expressionPromoter activityTrisphosphate receptorCyclase 6CRE elementTreatment of hepatocytesReceptor gene expressionAC isoformsCREBHormonal regulationProtein levelsIntracellular CaD. AnalysisPromoterRelease channelExpressionCyclic AMPIP3R2RegulationRat hepatocytes
2015
Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes
Weerachayaphorn J, Amaya MJ, Spirli C, Chansela P, Mitchell-Richards KA, Ananthanarayanan M, Nathanson MH. Nuclear Factor, Erythroid 2-Like 2 Regulates Expression of Type 3 Inositol 1,4,5-Trisphosphate Receptor and Calcium Signaling in Cholangiocytes. Gastroenterology 2015, 149: 211-222.e10. PMID: 25796361, PMCID: PMC4478166, DOI: 10.1053/j.gastro.2015.03.014.Peer-Reviewed Original ResearchConceptsBile ductBile duct unitsCholestatic disordersOxidative stressCalcium signalingNuclear factorMouse cholangiocytesDuct unitsReduced calcium signalingIntrahepatic bile ductsLevels of Nrf2Cholangiocyte cellsKnockdown of Nrf2Activation of Nrf2Intracellular calcium release channelsTranscription factor Nrf2Binding of Nrf2Calcium release channelPolymerase chain reaction analysisBiliary diseaseTrisphosphate receptorControl ratsLiver disordersBicarbonate secretionChain reaction analysis
2009
Nuclear factor erythroid 2–related factor 2 is a positive regulator of human bile salt export pump expression
Weerachayaphorn J, Cai S, Soroka CJ, Boyer JL. Nuclear factor erythroid 2–related factor 2 is a positive regulator of human bile salt export pump expression. Hepatology 2009, 50: 1588-1596. PMID: 19821532, PMCID: PMC3013376, DOI: 10.1002/hep.23151.Peer-Reviewed Original ResearchMeSH KeywordsATP Binding Cassette Transporter, Subfamily B, Member 11ATP-Binding Cassette TransportersBase SequenceHep G2 CellsHepatocytesHumansMaf Transcription FactorsMolecular Sequence DataNF-E2-Related Factor 2PyrazinesReverse Transcriptase InhibitorsRNA, MessengerSignal TransductionThionesThiophenesConceptsBile salt export pumpCholestatic liver injuryPositive transcriptional regulatorElectrophoretic mobility shift assaysHepG2 cellsBSEP expressionChromatin immunoprecipitation assaysBSEP promoterMobility shift assaysAdditional transcriptional factorsProximal promoter regionBSEP promoter activitySmall interfering RNAsFactor 2Liver injuryNuclear factorAntioxidant responsive elementTranscriptional regulatorsNrf2 transcriptional activationTranscriptional activationPositive regulatorBile salt export pump expressionNuclear farnesoid X receptorImmunoprecipitation assaysShift assays