2022
Molecular determinants of peri‐apical targeting of inositol 1,4,5‐trisphosphate receptor type 3 in cholangiocytes
Rodrigues MA, Gomes DA, Fiorotto R, Guerra MT, Weerachayaphorn J, Bo T, Sessa WC, Strazzabosco M, Nathanson MH. Molecular determinants of peri‐apical targeting of inositol 1,4,5‐trisphosphate receptor type 3 in cholangiocytes. Hepatology Communications 2022, 6: 2748-2764. PMID: 35852334, PMCID: PMC9512452, DOI: 10.1002/hep4.2042.Peer-Reviewed Original ResearchConceptsLipid raftsCaveolin-1Intact lipid raftsType 3 inositol trisphosphate receptorApical regionC-terminal amino acidsTrisphosphate receptor type 3Madin-Darby canine kidney cellsCanine kidney cellsFluorescence microscopy techniquesInositol trisphosphate receptorApical localizationTrisphosphate receptorHeavy chain 9Molecular determinantsChemical disruptionAmino acidsITPR3RaftsKidney cellsIntracellular CaFinal common eventReceptor type 3Release channelMYH9
2007
Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †
Weerachayaphorn J, Pajor AM. Threonine-509 Is a Determinant of Apparent Affinity for Both Substrate and Cations in the Human Na+/Dicarboxylate Cotransporter †. Biochemistry 2007, 47: 1087-1093. PMID: 18161988, PMCID: PMC2570185, DOI: 10.1021/bi701417h.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid SubstitutionAnimalsChlorocebus aethiopsCitric AcidCOS CellsDicarboxylic Acid TransportersGene ExpressionHumansKineticsMolecular Sequence DataMutationOrganic Anion Transporters, Sodium-DependentRabbitsRecombinant ProteinsSequence Homology, Amino AcidSodiumSubstrate SpecificitySuccinic AcidSymportersThreonineConceptsTransmembrane helix 7Functional differencesApparent affinityApparent sodium affinitySubstrate Binding SiteAmino acid differencesCitric acid cycleCitrate affinityHelix 7Substrate specificityPosition -509Rabbit sequenceIndividual residuesAcid cycleAcid differencesSimilar apparent affinitiesLoop regionIntermediate KmAmino acidsBinding sitesMutantsNaDC1SerineThreonineExhibit differencesSodium-dependent Extracellular Accessibility of Lys-84 in the Sodium/Dicarboxylate Cotransporter*
Weerachayaphorn J, Pajor AM. Sodium-dependent Extracellular Accessibility of Lys-84 in the Sodium/Dicarboxylate Cotransporter*. Journal Of Biological Chemistry 2007, 282: 20213-20220. PMID: 17504760, PMCID: PMC2864014, DOI: 10.1074/jbc.m701113200.Peer-Reviewed Original ResearchConceptsLys-84Transmembrane helicesMTS inhibitionLarge-scale conformational changesTranslocation of substratesCysteine-specific reagentsTransmembrane helix 3SLC13 familyCitric acid cycle intermediatesExtracellular halfEpithelial cell lineExtracellular accessibilityHelix 3Transport cycleTrp-103Human retinal pigment epithelial cell lineAddition of substrateConformational changesLeu-111Conformational statesRetinal pigment epithelial cell lineSubstrate affinityReentrant loopAmino acidsGlu-101