2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2019
Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions
Gong J, Cai H, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions. Stem Cells Translational Medicine 2019, 9: 364-376. PMID: 31840941, PMCID: PMC7031648, DOI: 10.1002/sctm.19-0321.Peer-Reviewed Original ResearchMeSH KeywordsBruch MembraneHumansInduced Pluripotent Stem CellsMacular DegenerationRetinal Pigment EpitheliumConceptsExtracellular matrixIPSC-RPEMetabolic-related pathwaysComplement immune systemTransepithelial electrical resistanceRod photoreceptor outer segmentsPluripotent stem cellsAged Bruch's membraneCell-specific morphologyObserved phenotypeAltered extracellular matrixControl iPSCsMitochondrial respirationMitochondrial functionMatrix interactionsCell attachmentStem cellsTranscriptomePhotoreceptor outer segmentsDistinct clustersComplement genesRetinal pigment epitheliumGenesIPSCsMembraneHigh-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD
Cai H, Gong J, Abriola L, Hoyer D, Team N, Noggle S, Paull D, Del Priore LV, Fields MA. High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD. Experimental Eye Research 2019, 185: 107641. PMID: 30980814, DOI: 10.1016/j.exer.2019.04.009.Peer-Reviewed Original ResearchMeSH KeywordsAntifungal AgentsApoptosisBasement MembraneCatalaseCell LineCiclopiroxCytoprotectionEpoxide HydrolasesGene Expression Regulation, EnzymologicGlutathione TransferaseHigh-Throughput Screening AssaysHumansInduced Pluripotent Stem CellsMacular DegenerationNitrosative StressOxidative StressPeroxiredoxin IIIReal-Time Polymerase Chain ReactionRetinal Pigment EpitheliumTert-ButylhydroperoxideUltraviolet RaysConceptsHigh-throughput screenHuman RPE cellsAtrophic age-related macular degenerationPrimary human RPE cellsMitochondrial functionStress-induced cell deathARPE-19Oxidative stress-induced cell deathExtracellular matrixTert-butyl hydroperoxide exposureRPE cellsOxidative damageExpression of genesInduced-pluripotent stem cellsOxidative stressSeahorse XF96 analyzerApoptosis-related genesCell viabilityGene expressionXF96 analyzerCell deathRetinal pigment epithelial cellsQuantitative reverse transcription polymerase chain reactionCiclopirox olaminePigment epithelial cells
2018
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
Cai H, Gong J, Del Priore LV, Tezel TH, Fields MA. Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane. Journal Of Visualized Experiments 2018, 57084. PMID: 29708536, PMCID: PMC5933494, DOI: 10.3791/57084.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overAgingBruch MembraneCell Culture TechniquesEpithelial CellsHumansMacular DegenerationPigment Epithelium of EyeRetinal PigmentsConceptsAtrophic age-related macular degenerationInduced pluripotent stem cellsRetinal pigment epithelial cellsEx vivo modelBruch's membranePigment epithelial cellsVivo modelGeographic atrophyHuman Bruch's membraneEstablishment of polarityHuman Bruch's membrane explantsRPE cellsAge-related macular degenerationMembrane pathologySufficient animal modelsEpithelial cellsEye Disease StudyPluripotent stem cellsAged Bruch's membraneBruch's membrane explantsPrimary cell linesAged human Bruch's membranePhotoreceptor outer segmentsGene expressionEffective therapy
2017
Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells
Fields MA, Bowrey HE, Gong J, Moreira EF, Cai H, Del Priore LV. Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells. PLOS ONE 2017, 12: e0177763. PMID: 28505174, PMCID: PMC5432172, DOI: 10.1371/journal.pone.0177763.Peer-Reviewed Original ResearchMeSH KeywordsAgedBiomarkersCell DifferentiationComplement ActivationComplement System ProteinsEpithelial CellsExtracellular MatrixEye ProteinsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMacular DegenerationNerve Growth FactorsRetinal Pigment EpitheliumSerpinsVascular Endothelial Growth Factor A
2015
Chapter Twenty-Eight Retinoid Processing in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cultures
Fields MA, Bowrey HE, Gong J, Ablonczy Z, Del Priore LV. Chapter Twenty-Eight Retinoid Processing in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cultures. Progress In Nucleic Acid Research And Molecular Biology 2015, 134: 477-490. PMID: 26310172, PMCID: PMC8680204, DOI: 10.1016/bs.pmbts.2015.06.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedHumansInduced Pluripotent Stem CellsMacular DegenerationRetinal Pigment EpitheliumRetinoidsVisual PathwaysConceptsRetinal pigment epitheliumRetinal degenerative diseasesAge-related macular degenerationDegenerative diseasesPluripotent stem cell (iPSC) technologyPromising clinical optionNative retinal pigment epitheliumRetinal degenerative disordersStem cell-derived retinal pigment epitheliumStem cell technologyVisual functionMacular degenerationPigment epitheliumRetinoid processingClinical optionDegenerative disordersClinical therapyCell therapyPluripotent stem cell-derived retinal pigment epitheliumVisual cycleRPE-specific proteinTherapyDiseaseStem cellsTrans retinol