2018
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
Cai H, Gong J, Del Priore LV, Tezel TH, Fields MA. Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane. Journal Of Visualized Experiments 2018, 57084. PMID: 29708536, PMCID: PMC5933494, DOI: 10.3791/57084.Peer-Reviewed Original ResearchConceptsAtrophic age-related macular degenerationInduced pluripotent stem cellsRetinal pigment epithelial cellsEx vivo modelBruch's membranePigment epithelial cellsVivo modelGeographic atrophyHuman Bruch's membraneEstablishment of polarityHuman Bruch's membrane explantsRPE cellsAge-related macular degenerationMembrane pathologySufficient animal modelsEpithelial cellsEye Disease StudyPluripotent stem cellsAged Bruch's membraneBruch's membrane explantsPrimary cell linesAged human Bruch's membranePhotoreceptor outer segmentsGene expressionEffective therapy
2017
Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells
Fields MA, Bowrey HE, Gong J, Moreira EF, Cai H, Del Priore LV. Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells. PLOS ONE 2017, 12: e0177763. PMID: 28505174, PMCID: PMC5432172, DOI: 10.1371/journal.pone.0177763.Peer-Reviewed Original ResearchMeSH KeywordsAgedBiomarkersCell DifferentiationComplement ActivationComplement System ProteinsEpithelial CellsExtracellular MatrixEye ProteinsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMacular DegenerationNerve Growth FactorsRetinal Pigment EpitheliumSerpinsVascular Endothelial Growth Factor A