2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2017
Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells
Fields MA, Bowrey HE, Gong J, Moreira EF, Cai H, Del Priore LV. Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells. PLOS ONE 2017, 12: e0177763. PMID: 28505174, PMCID: PMC5432172, DOI: 10.1371/journal.pone.0177763.Peer-Reviewed Original ResearchMeSH KeywordsAgedBiomarkersCell DifferentiationComplement ActivationComplement System ProteinsEpithelial CellsExtracellular MatrixEye ProteinsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMacular DegenerationNerve Growth FactorsRetinal Pigment EpitheliumSerpinsVascular Endothelial Growth Factor A
2015
Differentiation of Human Protein-Induced Pluripotent Stem Cells toward a Retinal Pigment Epithelial Cell Fate
Gong J, Fields MA, Moreira EF, Bowrey HE, Gooz M, Ablonczy Z, Del Priore LV. Differentiation of Human Protein-Induced Pluripotent Stem Cells toward a Retinal Pigment Epithelial Cell Fate. PLOS ONE 2015, 10: e0143272. PMID: 26606685, PMCID: PMC4659559, DOI: 10.1371/journal.pone.0143272.Peer-Reviewed Original Research
2008
Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors
Gong J, Sagiv O, Cai H, Tsang SH, Del Priore LV. Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors. Experimental Eye Research 2008, 86: 957-965. PMID: 18472095, PMCID: PMC4405535, DOI: 10.1016/j.exer.2008.03.014.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsEmbryonic stem cellsNeural progenitorsNeural progenitor markersPA6 cellsStem cellsRPE markersProgenitor markersExtracellular matrixHuman Bruch's membrane explantsNeighboring cellsGene expression profilesNervous system developmentBruch's membrane explantsPA6 stromal cellsNew genesNeural retinaAdult neural retinaExpression profilesMicroarray analysisEpithelial progenitorsInduced expressionCell adhesionNeurite formationNeuronal phenotype