2021
Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium
Cai H, Gong J, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Altered transcriptome and disease-related phenotype emerge only after fibroblasts harvested from patients with age-related macular degeneration are differentiated into retinal pigment epithelium. Experimental Eye Research 2021, 207: 108576. PMID: 33895162, DOI: 10.1016/j.exer.2021.108576.Peer-Reviewed Original ResearchConceptsAge-related macular degenerationRetinal pigment epitheliumMacular degenerationPigment epitheliumInduced pluripotent stem cellsEtiology of AMDMitochondrial dysfunctionAge-matched controlsNovel therapeutic targetTranscriptome of fibroblastsAMD patientsNormal donorsFibroblasts of patientsTherapeutic targetPatientsMore studiesAltered transcriptomeDisease phenotypeSignificant differencesCell linesMitochondrial functionDysfunctionOriginal fibroblastsDistinct transcriptomesDegeneration
2019
Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions
Gong J, Cai H, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions. Stem Cells Translational Medicine 2019, 9: 364-376. PMID: 31840941, PMCID: PMC7031648, DOI: 10.1002/sctm.19-0321.Peer-Reviewed Original ResearchConceptsExtracellular matrixIPSC-RPEMetabolic-related pathwaysComplement immune systemTransepithelial electrical resistanceRod photoreceptor outer segmentsPluripotent stem cellsAged Bruch's membraneCell-specific morphologyObserved phenotypeAltered extracellular matrixControl iPSCsMitochondrial respirationMitochondrial functionMatrix interactionsCell attachmentStem cellsTranscriptomePhotoreceptor outer segmentsDistinct clustersComplement genesRetinal pigment epitheliumGenesIPSCsMembraneHigh-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD
Cai H, Gong J, Abriola L, Hoyer D, Team N, Noggle S, Paull D, Del Priore LV, Fields MA. High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD. Experimental Eye Research 2019, 185: 107641. PMID: 30980814, DOI: 10.1016/j.exer.2019.04.009.Peer-Reviewed Original ResearchMeSH KeywordsAntifungal AgentsApoptosisBasement MembraneCatalaseCell LineCiclopiroxCytoprotectionEpoxide HydrolasesGene Expression Regulation, EnzymologicGlutathione TransferaseHigh-Throughput Screening AssaysHumansInduced Pluripotent Stem CellsMacular DegenerationNitrosative StressOxidative StressPeroxiredoxin IIIReal-Time Polymerase Chain ReactionRetinal Pigment EpitheliumTert-ButylhydroperoxideUltraviolet RaysConceptsHigh-throughput screenHuman RPE cellsAtrophic age-related macular degenerationPrimary human RPE cellsMitochondrial functionStress-induced cell deathARPE-19Oxidative stress-induced cell deathExtracellular matrixTert-butyl hydroperoxide exposureRPE cellsOxidative damageExpression of genesInduced-pluripotent stem cellsOxidative stressSeahorse XF96 analyzerApoptosis-related genesCell viabilityGene expressionXF96 analyzerCell deathRetinal pigment epithelial cellsQuantitative reverse transcription polymerase chain reactionCiclopirox olaminePigment epithelial cells