2019
Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions
Gong J, Cai H, Team N, Noggle S, Paull D, Rizzolo LJ, Del Priore LV, Fields MA. Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions. Stem Cells Translational Medicine 2019, 9: 364-376. PMID: 31840941, PMCID: PMC7031648, DOI: 10.1002/sctm.19-0321.Peer-Reviewed Original ResearchConceptsExtracellular matrixIPSC-RPEMetabolic-related pathwaysComplement immune systemTransepithelial electrical resistanceRod photoreceptor outer segmentsPluripotent stem cellsAged Bruch's membraneCell-specific morphologyObserved phenotypeAltered extracellular matrixControl iPSCsMitochondrial respirationMitochondrial functionMatrix interactionsCell attachmentStem cellsTranscriptomePhotoreceptor outer segmentsDistinct clustersComplement genesRetinal pigment epitheliumGenesIPSCsMembraneHigh-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD
Cai H, Gong J, Abriola L, Hoyer D, Team N, Noggle S, Paull D, Del Priore LV, Fields MA. High-throughput screening identifies compounds that protect RPE cells from physiological stressors present in AMD. Experimental Eye Research 2019, 185: 107641. PMID: 30980814, DOI: 10.1016/j.exer.2019.04.009.Peer-Reviewed Original ResearchMeSH KeywordsAntifungal AgentsApoptosisBasement MembraneCatalaseCell LineCiclopiroxCytoprotectionEpoxide HydrolasesGene Expression Regulation, EnzymologicGlutathione TransferaseHigh-Throughput Screening AssaysHumansInduced Pluripotent Stem CellsMacular DegenerationNitrosative StressOxidative StressPeroxiredoxin IIIReal-Time Polymerase Chain ReactionRetinal Pigment EpitheliumTert-ButylhydroperoxideUltraviolet RaysConceptsHigh-throughput screenHuman RPE cellsAtrophic age-related macular degenerationPrimary human RPE cellsMitochondrial functionStress-induced cell deathARPE-19Oxidative stress-induced cell deathExtracellular matrixTert-butyl hydroperoxide exposureRPE cellsOxidative damageExpression of genesInduced-pluripotent stem cellsOxidative stressSeahorse XF96 analyzerApoptosis-related genesCell viabilityGene expressionXF96 analyzerCell deathRetinal pigment epithelial cellsQuantitative reverse transcription polymerase chain reactionCiclopirox olaminePigment epithelial cells
2017
Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells
Fields MA, Bowrey HE, Gong J, Moreira EF, Cai H, Del Priore LV. Extracellular matrix nitration alters growth factor release and activates bioactive complement in human retinal pigment epithelial cells. PLOS ONE 2017, 12: e0177763. PMID: 28505174, PMCID: PMC5432172, DOI: 10.1371/journal.pone.0177763.Peer-Reviewed Original ResearchMeSH KeywordsAgedBiomarkersCell DifferentiationComplement ActivationComplement System ProteinsEpithelial CellsExtracellular MatrixEye ProteinsFemaleFibroblastsHumansInduced Pluripotent Stem CellsMacular DegenerationNerve Growth FactorsRetinal Pigment EpitheliumSerpinsVascular Endothelial Growth Factor A
2008
Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors
Gong J, Sagiv O, Cai H, Tsang SH, Del Priore LV. Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors. Experimental Eye Research 2008, 86: 957-965. PMID: 18472095, PMCID: PMC4405535, DOI: 10.1016/j.exer.2008.03.014.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsEmbryonic stem cellsNeural progenitorsNeural progenitor markersPA6 cellsStem cellsRPE markersProgenitor markersExtracellular matrixHuman Bruch's membrane explantsNeighboring cellsGene expression profilesNervous system developmentBruch's membrane explantsPA6 stromal cellsNew genesNeural retinaAdult neural retinaExpression profilesMicroarray analysisEpithelial progenitorsInduced expressionCell adhesionNeurite formationNeuronal phenotype