2021
Structural insights into the cause of human RSPH4A primary ciliary dyskinesia
Zhao Y, Pinskey J, Lin J, Yin W, Sears P, Daniels L, Zariwala M, Knowles M, Ostrowski L, Nicastro D. Structural insights into the cause of human RSPH4A primary ciliary dyskinesia. Molecular Biology Of The Cell 2021, 32: 1202-1209. PMID: 33852348, PMCID: PMC8351563, DOI: 10.1091/mbc.e20-12-0806.Peer-Reviewed Original ResearchConceptsStructural basisCryo-electron tomographyRadial spokesCentral pair complexUnderlying structural basisAxonemal repeatEukaryotic organellesArch domainThree-dimensional structureSubtomogram averagingOrgan positioningCell motilityStructural insightsPrimary ciliary dyskinesiaCiliaHuman ciliaHuman respiratory ciliaRS1Primary defectStructure determinationCiliary dyskinesiaHuman healthOrganellesFlagellaRepeats
2020
Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia
Liu P, Lou X, Wingfield JL, Lin J, Nicastro D, Lechtreck K. Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia. Journal Of Cell Biology 2020, 219: e202001122. PMID: 32348466, PMCID: PMC7265326, DOI: 10.1083/jcb.202001122.Peer-Reviewed Original Research
2019
FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O’Toole E, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Molecular Biology Of The Cell 2019, 30: 2659-2680. PMID: 31483737, PMCID: PMC6761771, DOI: 10.1091/mbc.e19-07-0367.Peer-Reviewed Original ResearchConceptsInner dynein armsRegulatory complexCryo-electron tomographyInner arm dyneinsCiliary motilityMultiple dynein motorsPrecise spatial organizationAxonemal repeatDocking factorUnique binding siteWD repeatsDynein assemblyAssembly factorsDomain proteinsRegulatory hubDynein complexDynein isoformsDynein subunitsInsertional mutagenesisNew lociRegulatory proteinsDynein motorsDifferent dyneinsDoublet microtubulesTransport factorsPACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility
Dymek EE, Lin J, Fu G, Porter ME, Nicastro D, Smith EF. PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. Molecular Biology Of The Cell 2019, 30: 1805-1816. PMID: 31116684, PMCID: PMC6727744, DOI: 10.1091/mbc.e19-01-0063.Peer-Reviewed Original ResearchMeSH KeywordsAlgal ProteinsAxonemeChlamydomonas reinhardtiiCiliaFlagellaMicrotubulesMovementMutationConceptsDoublet microtubulesAxonemal doublet microtubulesCryo-electron tomographyCiliary doublet microtubulesInner junctionCiliary assemblyCoordinated ciliary beatingFAP20Ciliary componentsMotility defectsCiliary beatingPACRGMotile ciliaFunctional studiesMutant axonemesMicrotubulesB-tubuleCiliary motilityEssential roleStructural studiesAssemblyMotilityChlamydomonasMutantsAxoneme
2012
Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein
Heuser T, Barber CF, Lin J, Krell J, Rebesco M, Porter ME, Nicastro D. Cryoelectron tomography reveals doublet-specific structures and unique interactions in the I1 dynein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: e2067-e2076. PMID: 22733763, PMCID: PMC3409752, DOI: 10.1073/pnas.1120690109.Peer-Reviewed Original ResearchConceptsI1 dyneinCryoelectron tomographyGel-based proteomicsInner arm dyneinsCiliary assemblySensory organellesSubtomogram averagingDynein activityCritical regulatorDyneinPolypeptide compositionDoublet microtubulesBiochemical comparisonMotor domainCiliary motilityFlagellar beatingUnique interactionsCiliary beatingMotilityEukaryotesAssemblyChlamydomonasSubcomplexMutantsCiliopathies
2011
Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*
Lin J, Tritschler D, Song K, Barber CF, Cobb JS, Porter ME, Nicastro D. Building Blocks of the Nexin-Dynein Regulatory Complex in Chlamydomonas Flagella*. Journal Of Biological Chemistry 2011, 286: 29175-29191. PMID: 21700706, PMCID: PMC3190725, DOI: 10.1074/jbc.m111.241760.Peer-Reviewed Original ResearchConceptsNexin-dynein regulatory complexRegulatory complexImportant regulatory nodeTwo-dimensional electrophoresisRegulatory nodesProtein phosphorylationSignal transductionPhosphorylation statusMALDI-TOF mass spectrometryDynein motorsDynein activityChlamydomonas flagellaMotile ciliaRegulatory functionsPhosphorylated isoformsCoordination of thousandsFlagellar axonemeBiochemical comparisonRadial spokesPCR analysisFlagellar bendingIsoform patternFlagellaMolecular compositionProtein