2018
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility
Lin J, Nicastro D. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Science 2018, 360 PMID: 29700238, PMCID: PMC6640125, DOI: 10.1126/science.aar1968.Peer-Reviewed Original ResearchConceptsDynein activitySea urchin sperm cellsCryo-electron tomographyIndividual dyneinsDynein isoformsAsymmetric distributionMotile ciliaDyneinFlagellaHypothesis positsSperm cellsSmall populationCiliary motilityFlagellar bendingCoordinated activityActive stateMotilityActivity stateOrganellesIsoformsCiliaActivityThe I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility
Fu G, Wang Q, Phan N, Urbanska P, Joachimiak E, Lin J, Wloga D, Nicastro D. The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology Of The Cell 2018, 29: mbc.e18-02-0142. PMID: 29514928, PMCID: PMC5921572, DOI: 10.1091/mbc.e18-02-0142.Peer-Reviewed Original ResearchConceptsI1 dyneinCiliary motilityAxonemal dynein motorsDynein motor domainMotor domainCiliary doublet microtubulesCK1 kinasesProper phosphorylationNormal ciliary motilityBiochemical approachesCryoelectron tomographyDynein motorsRegulatory mechanismsMotility regulatorMotile ciliaDoublet microtubulesRegulatorDyneinStable anchoringMotilityCiliary beatingComplexesHead complexMechanical feedbackImportant role
2014
Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦
Linck R, Fu X, Lin J, Ouch C, Schefter A, Steffen W, Warren P, Nicastro D. Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦. Journal Of Biological Chemistry 2014, 289: 17427-17444. PMID: 24794867, PMCID: PMC4067180, DOI: 10.1074/jbc.m114.568949.Peer-Reviewed Original ResearchConceptsDoublet microtubulesRibbon proteinNexin-dynein regulatory complexCiliary motilityA-tubuleIntraflagellar transportRegulatory complexAcetylated α-tubulinSignal transductionHuman homologueSpecialized functionsTektin ACalcium-binding proteinsCell organellesHuman diseasesBinding proteinΑ-tubulinΒ-tubulinSea urchin sperm flagellaSperm flagellaMicrotubule systemProteinStable protofilamentsFlagellaWide filamentsStructural mechanism of the dynein power stroke
Lin J, Okada K, Raytchev M, Smith MC, Nicastro D. Structural mechanism of the dynein power stroke. Nature Cell Biology 2014, 16: 479-485. PMID: 24727830, PMCID: PMC4102432, DOI: 10.1038/ncb2939.Peer-Reviewed Original Research
2012
Conserved structural motifs in the central pair complex of eukaryotic flagella
Carbajal‐González B, Heuser T, Fu X, Lin J, Smith BW, Mitchell DR, Nicastro D. Conserved structural motifs in the central pair complex of eukaryotic flagella. Cytoskeleton 2012, 70: 101-120. PMID: 23281266, PMCID: PMC3914236, DOI: 10.1002/cm.21094.Peer-Reviewed Original ResearchConceptsCentral pair complexCilia/flagellaRemarkable structural conservationGreen alga ChlamydomonasConserved Structural MotifCryo-electron tomographyOrganism-specific differencesEukaryotic cellsSea urchin StrongylocentrotusEvolutionary distanceStructural conservationAlga ChlamydomonasThree-dimensional structureSubtomogram averagingEukaryotic flagellaAxonemal dyneinsPrecise regulationInner proteinsMotility regulatorHair-like appendagesSinglet microtubulesChlamydomonasHuman diseasesDoublet microtubulesFlagellaOne of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures
Lin J, Heuser T, Song K, Fu X, Nicastro D. One of the Nine Doublet Microtubules of Eukaryotic Flagella Exhibits Unique and Partially Conserved Structures. PLOS ONE 2012, 7: e46494. PMID: 23071579, PMCID: PMC3468612, DOI: 10.1371/journal.pone.0046494.Peer-Reviewed Original ResearchThe structural heterogeneity of radial spokes in cilia and flagella is conserved
Lin J, Heuser T, Carbajal‐González B, Song K, Nicastro D. The structural heterogeneity of radial spokes in cilia and flagella is conserved. Cytoskeleton 2012, 69: 88-100. PMID: 22170736, PMCID: PMC3307805, DOI: 10.1002/cm.21000.Peer-Reviewed Original ResearchConceptsFlagellar motilityRadial spokesNexin-dynein regulatory complexRegulatory complexFlagellar structureMost organismsThree-dimensional structureSubtomogram averagingCryoelectron tomographyDynein motorsDynein activityChlamydomonas flagellaMotile ciliaFlagellaSperm flagellaUbiquitous componentOrganismsFunctional differencesEssential roleCiliaRepeat unitsUnprecedented detailBasal partRS1Motility