Jianfeng Lin, PhD
Senior Research ScientistCards
About
Research
Publications
2021
Structural insights into the cause of human RSPH4A primary ciliary dyskinesia
Zhao Y, Pinskey J, Lin J, Yin W, Sears P, Daniels L, Zariwala M, Knowles M, Ostrowski L, Nicastro D. Structural insights into the cause of human RSPH4A primary ciliary dyskinesia. Molecular Biology Of The Cell 2021, 32: 1202-1209. PMID: 33852348, PMCID: PMC8351563, DOI: 10.1091/mbc.e20-12-0806.Peer-Reviewed Original ResearchConceptsStructural basisCryo-electron tomographyRadial spokesCentral pair complexUnderlying structural basisAxonemal repeatEukaryotic organellesArch domainThree-dimensional structureSubtomogram averagingOrgan positioningCell motilityStructural insightsPrimary ciliary dyskinesiaCiliaHuman ciliaHuman respiratory ciliaRS1Primary defectStructure determinationCiliary dyskinesiaHuman healthOrganellesFlagellaRepeats
2020
Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia
Liu P, Lou X, Wingfield JL, Lin J, Nicastro D, Lechtreck K. Chlamydomonas PKD2 organizes mastigonemes, hair-like glycoprotein polymers on cilia. Journal Of Cell Biology 2020, 219: e202001122. PMID: 32348466, PMCID: PMC7265326, DOI: 10.1083/jcb.202001122.Peer-Reviewed Original Research
2019
FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia
Lin J, Le TV, Augspurger K, Tritschler D, Bower R, Fu G, Perrone C, O’Toole E, Mills KV, Dymek E, Smith E, Nicastro D, Porter ME. FAP57/WDR65 targets assembly of a subset of inner arm dyneins and connects to regulatory hubs in cilia. Molecular Biology Of The Cell 2019, 30: 2659-2680. PMID: 31483737, PMCID: PMC6761771, DOI: 10.1091/mbc.e19-07-0367.Peer-Reviewed Original ResearchConceptsInner dynein armsRegulatory complexCryo-electron tomographyInner arm dyneinsCiliary motilityMultiple dynein motorsPrecise spatial organizationAxonemal repeatDocking factorUnique binding siteWD repeatsDynein assemblyAssembly factorsDomain proteinsRegulatory hubDynein complexDynein isoformsDynein subunitsInsertional mutagenesisNew lociRegulatory proteinsDynein motorsDifferent dyneinsDoublet microtubulesTransport factorsPACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility
Dymek EE, Lin J, Fu G, Porter ME, Nicastro D, Smith EF. PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. Molecular Biology Of The Cell 2019, 30: 1805-1816. PMID: 31116684, PMCID: PMC6727744, DOI: 10.1091/mbc.e19-01-0063.Peer-Reviewed Original ResearchConceptsDoublet microtubulesAxonemal doublet microtubulesCryo-electron tomographyCiliary doublet microtubulesInner junctionCiliary assemblyCoordinated ciliary beatingFAP20Ciliary componentsMotility defectsCiliary beatingPACRGMotile ciliaFunctional studiesMutant axonemesMicrotubulesB-tubuleCiliary motilityEssential roleStructural studiesAssemblyMotilityChlamydomonasMutantsAxoneme
2018
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility
Lin J, Nicastro D. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility. Science 2018, 360 PMID: 29700238, PMCID: PMC6640125, DOI: 10.1126/science.aar1968.Peer-Reviewed Original ResearchConceptsDynein activitySea urchin sperm cellsCryo-electron tomographyIndividual dyneinsDynein isoformsAsymmetric distributionMotile ciliaDyneinFlagellaHypothesis positsSperm cellsSmall populationCiliary motilityFlagellar bendingCoordinated activityActive stateMotilityActivity stateOrganellesIsoformsCiliaActivityThe I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility
Fu G, Wang Q, Phan N, Urbanska P, Joachimiak E, Lin J, Wloga D, Nicastro D. The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology Of The Cell 2018, 29: mbc.e18-02-0142. PMID: 29514928, PMCID: PMC5921572, DOI: 10.1091/mbc.e18-02-0142.Peer-Reviewed Original ResearchConceptsI1 dyneinCiliary motilityAxonemal dynein motorsDynein motor domainMotor domainCiliary doublet microtubulesCK1 kinasesProper phosphorylationNormal ciliary motilityBiochemical approachesCryoelectron tomographyDynein motorsRegulatory mechanismsMotility regulatorMotile ciliaDoublet microtubulesRegulatorDyneinStable anchoringMotilityCiliary beatingComplexesHead complexMechanical feedbackImportant role
2015
DRC3 connects the N-DRC to dynein g to regulate flagellar waveform
Awata J, Song K, Lin J, King SM, Sanderson MJ, Nicastro D, Witman GB. DRC3 connects the N-DRC to dynein g to regulate flagellar waveform. Molecular Biology Of The Cell 2015, 26: 2788-2800. PMID: 26063732, PMCID: PMC4571338, DOI: 10.1091/mbc.e15-01-0018.Peer-Reviewed Original Research
2014
Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia
Lin J, Yin W, Smith MC, Song K, Leigh MW, Zariwala MA, Knowles MR, Ostrowski LE, Nicastro D. Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia. Nature Communications 2014, 5: 5727. PMID: 25473808, PMCID: PMC4267722, DOI: 10.1038/ncomms6727.Peer-Reviewed Original ResearchConceptsCryo-electron tomographyNative 3D structureNormal human developmentDistant speciesHuman ciliaCilia structurePCD phenotypeCilia dysfunctionHuman diseasesNative structurePrimary ciliary dyskinesiaRadial spokesCiliaEssential roleFunctional heterogeneityHuman respiratory ciliaUnprecedented detailPrimary defectCiliary dyskinesiaUnknown primary defectInsights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦
Linck R, Fu X, Lin J, Ouch C, Schefter A, Steffen W, Warren P, Nicastro D. Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules TEKTINS, Ca2+-BINDING PROTEINS, AND STABLE PROTOFILAMENTS* ♦. Journal Of Biological Chemistry 2014, 289: 17427-17444. PMID: 24794867, PMCID: PMC4067180, DOI: 10.1074/jbc.m114.568949.Peer-Reviewed Original ResearchConceptsDoublet microtubulesRibbon proteinNexin-dynein regulatory complexCiliary motilityA-tubuleIntraflagellar transportRegulatory complexAcetylated α-tubulinSignal transductionHuman homologueSpecialized functionsTektin ACalcium-binding proteinsCell organellesHuman diseasesBinding proteinΑ-tubulinΒ-tubulinSea urchin sperm flagellaSperm flagellaMicrotubule systemProteinStable protofilamentsFlagellaWide filamentsStructural mechanism of the dynein power stroke
Lin J, Okada K, Raytchev M, Smith MC, Nicastro D. Structural mechanism of the dynein power stroke. Nature Cell Biology 2014, 16: 479-485. PMID: 24727830, PMCID: PMC4102432, DOI: 10.1038/ncb2939.Peer-Reviewed Original Research