2024
Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging
Milicevic K, Ivanova V, Lovic D, Platisa J, Andjus P, Antic S. Plateau depolarizations in spontaneously active neurons detected by calcium or voltage imaging. Scientific Reports 2024, 14: 22787. PMID: 39367010, PMCID: PMC11452489, DOI: 10.1038/s41598-024-70319-4.Peer-Reviewed Original ResearchConceptsCa2+ transientsAction potentialsSpontaneously active neuronsCalcium imaging studiesCa2+ recordingsNeuronal action potentialsDepolarizing envelopeOptical signalsDendritic Ca2AP firingPlateau depolarizationsPyramidal neuronsMembrane depolarizationPlateau potentialsImaging studiesActive neuronsNeuronal culturesPhoton signaturesImaging modalitiesUP statesIn vivo conditionsNeuronsHalf-widthCa2+Depolarization
2023
High-speed low-light in vivo two-photon voltage imaging of large neuronal populations
Platisa J, Ye X, Ahrens A, Liu C, Chen I, Davison I, Tian L, Pieribone V, Chen J. High-speed low-light in vivo two-photon voltage imaging of large neuronal populations. Nature Methods 2023, 20: 1095-1103. PMID: 36973547, PMCID: PMC10894646, DOI: 10.1038/s41592-023-01820-3.Peer-Reviewed Original ResearchConceptsNeuronal populationsLarge neuronal populationsNeural circuit functionTwo-photon voltage imagingAwake behaving miceCalcium imagingBehaving miceVoltage imagingCircuit functionNeuronsShot noise levelShot-noise limitImagingTwo-photon microscopeKilohertz frame ratesDeep-tissue imagingPopulationVoltage indicatorsField of viewMiceShot noise
2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2013
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLOS ONE 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.Peer-Reviewed Original Research
2012
Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals
Osman A, Park JH, Dickensheets D, Platisa J, Culurciello E, Pieribone VA. Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals. IEEE Transactions On Biomedical Circuits And Systems 2012, 6: 446-453. PMID: 23853231, DOI: 10.1109/tbcas.2012.2226174.Peer-Reviewed Original ResearchA Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original Research