2023
Connexin 43-mediated neurovascular interactions regulate neurogenesis in the adult brain subventricular zone
Genet N, Genet G, Chavkin N, Paila U, Fang J, Vasavada H, Goldberg J, Acharya B, Bhatt N, Baker K, McDonnell S, Huba M, Sankaranarayanan D, Ma G, Eichmann A, Thomas J, Ffrench-Constant C, Hirschi K. Connexin 43-mediated neurovascular interactions regulate neurogenesis in the adult brain subventricular zone. Cell Reports 2023, 42: 112371. PMID: 37043357, PMCID: PMC10564973, DOI: 10.1016/j.celrep.2023.112371.Peer-Reviewed Original ResearchConceptsSubventricular zoneEndothelial cellsNSC proliferationNSC quiescenceAdult brain subventricular zoneBlood-brain barrierBrain subventricular zoneStem cell therapyNeural stem cell nicheNeurovascular interactionsNeuroblast generationAdult brainSingle-cell RNA sequencingNeurodegenerative disordersChannel-independent mannerCell therapySVZ-NSCsConnexin 43Expression of genesERK activationNSC regulationNSC behaviorRNA sequencingCx43Proliferation
2018
Orchestrating cortical brain development
Thomas JL. Orchestrating cortical brain development. Science 2018, 361: 754-755. PMID: 30139860, DOI: 10.1126/science.aau7155.Peer-Reviewed Original ResearchConceptsCentral nervous systemBrain developmentEndothelial cellsCentral nervous system morphologyCortical brain developmentNeuronal cell componentsProper brain developmentNervous system morphologyOwn vascular networkGlial cellsNervous systemBlood vesselsNeural tissueVasculatureVascular networkCellsCell componentsNeuronsAdult Human Hippocampus: No New Neurons in Sight
Arellano JI, Harding B, Thomas JL. Adult Human Hippocampus: No New Neurons in Sight. Cerebral Cortex 2018, 28: 2479-2481. PMID: 29746611, DOI: 10.1093/cercor/bhy106.Peer-Reviewed Original Research
2016
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres.
Lopez-Ramirez MA, Calvo CF, Ristori E, Thomas JL, Nicoli S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. Journal Of Visualized Experiments 2016, 53617. PMID: 26967835, PMCID: PMC4828210, DOI: 10.3791/53617.Peer-Reviewed Original ResearchConceptsAdult zebrafish brainStem/progenitor cellsZebrafish brainMolecular mechanismsRelevant model organismZebrafish adult brainProgenitor cellsSingle-cell dissociationSpecific signaling pathwaysAdult stem/progenitor cellsStem/progenitor cell proliferationNeural stem/progenitor cell proliferationProgenitor cell proliferationNeural stem/progenitor cellsModel organismsGene expressionCell dissociationSignaling pathwaysMultipotent neural stem/progenitor cellsNeurosphere assayAdult neurogenesisCell proliferationZebrafishBrain regenerationAdult brain
2015
Embryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress
Ge XQ, Han J, Cheng EC, Yamaguchi S, Shima N, Thomas JL, Lin H. Embryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress. Stem Cell Reports 2015, 5: 185-194. PMID: 26190528, PMCID: PMC4618655, DOI: 10.1016/j.stemcr.2015.06.002.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsStem/progenitor cellsNeural stem/progenitor cellsStem cellsProgenitor cellsTissue stem/progenitor cellsMCM2-7 complexDNA replication originsTissue-specific stem/progenitor cellsStem cell typesGenome integrityGenomic integrityReplication stressDormant originsReplication forksReplicative stressDNA replicationReplication originsNeural lineagesDNA damageS phaseCell typesAbnormal neurogenesisCellsGenomeVascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans
Han J, Calvo CF, Kang TH, Baker KL, Park JH, Parras C, Levittas M, Birba U, Pibouin-Fragner L, Fragner P, Bilguvar K, Duman RS, Nurmi H, Alitalo K, Eichmann AC, Thomas JL. Vascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans. Cell Reports 2015, 10: 1158-1172. PMID: 25704818, PMCID: PMC4685253, DOI: 10.1016/j.celrep.2015.01.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell ProliferationCells, CulturedEmbryonic Stem CellsExtracellular Signal-Regulated MAP KinasesHippocampusHumansMiceMice, Inbred C57BLNeural Stem CellsNeurogenesisProto-Oncogene Proteins c-aktRecombinant ProteinsSignal TransductionVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3ConceptsHuman embryonic stem cellsNeural stem cellsVascular endothelial growth factor receptor 3Growth factor receptor 3NSC activationStem cellsProgenitor cellsAdult hippocampal neural stem cellsEmbryonic stem cellsNeural stem cell activationStem cell activationQuiescent neural stem cellsNeural progenitor cellsCell fateReceptor 3Specific regulatorsAdult mammalian hippocampusMolecular mechanismsCell cycleHippocampal neural stem cellsLigand VEGFERK pathwayConditional deletionNew neuronsVEGFR3A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis
Ristori E, Lopez-Ramirez MA, Narayanan A, Hill-Teran G, Moro A, Calvo CF, Thomas JL, Nicoli S. A Dicer-miR-107 Interaction Regulates Biogenesis of Specific miRNAs Crucial for Neurogenesis. Developmental Cell 2015, 32: 546-560. PMID: 25662174, PMCID: PMC8950125, DOI: 10.1016/j.devcel.2014.12.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell DifferentiationCell ProliferationImmunoenzyme TechniquesIn Situ HybridizationMicroRNAsNeurogenesisNeuronsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionRhombencephalonRibonuclease IIIRNA, MessengerTumor Cells, CulturedZebrafishZebrafish ProteinsConceptsSpecific miRNAsDicer levelsBiogenesis of microRNAsNeuronal cellsDicer expression levelsExpression levelsDicer resultsMiRNA biogenesisMiR-107 functionsBiogenesisEctopic accumulationSubstrate selectivityPostmitotic neuronsMiR-107MiR-9MiRNAsHomeostatic levelsPrecise accumulationNeurogenesisDicerCellsAccumulationMicroRNAsRecent reportsProgenitors
2013
The power of VEGF (vascular endothelial growth factor) family molecules
Thomas JL, Eichmann A. The power of VEGF (vascular endothelial growth factor) family molecules. Cellular And Molecular Life Sciences 2013, 70: 1673-1674. PMID: 23475064, PMCID: PMC11113691, DOI: 10.1007/s00018-013-1276-6.Peer-Reviewed Original ResearchInteractions between VEGFR and Notch signaling pathways in endothelial and neural cells
Thomas JL, Baker K, Han J, Calvo C, Nurmi H, Eichmann AC, Alitalo K. Interactions between VEGFR and Notch signaling pathways in endothelial and neural cells. Cellular And Molecular Life Sciences 2013, 70: 1779-1792. PMID: 23479133, PMCID: PMC3648205, DOI: 10.1007/s00018-013-1312-6.Peer-Reviewed Original ResearchConceptsCell fate decisionsDifferent cell contextsTyrosine kinase VEGF receptorsExtracellular matrix moleculesCell interaction mechanismsMetazoan speciesFate decisionsGrowth factorCanonical NotchVascular endothelial growth factorNotch receptorsCell contextEnvironmental cuesDynamic regulationMolecular relationshipsKey regulatorNotch pathwayMatrix moleculesNeural cellsCell contactAdjacent cellsPathwayVEGF receptorsCellsNeurovascular interactions
2011
A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells
Lamprianou S, Chatzopoulou E, Thomas J, Bouyain S, Harroch S. A complex between contactin-1 and the protein tyrosine phosphatase PTPRZ controls the development of oligodendrocyte precursor cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 17498-17503. PMID: 21969550, PMCID: PMC3198311, DOI: 10.1073/pnas.1108774108.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCell DifferentiationCell ProliferationContactin 1Crystallography, X-RayHumansMiceMice, KnockoutModels, MolecularModels, NeurologicalMultiprotein ComplexesNeural Stem CellsNeurogenesisOligodendrogliaProtein Structure, TertiaryReceptor-Like Protein Tyrosine Phosphatases, Class 5Recombinant ProteinsSolubilityConceptsCarbonic anhydrase-like domainPrecursor cellsReceptor protein tyrosineOligodendrocyte precursor cellsPtprz-deficient miceProtein tyrosineCell adhesion moleculeNeural cell adhesion moleculeBiological roleContactin familyCocrystal structureGlial cell populationsUnknown modulatorsPtprzCentral nervous systemCell populationsCNTN1Structural dataAdhesion moleculesBindsContactin-1CellsMature oligodendrocytesComplexesNervous systemVascular endothelial growth factor receptor 3 directly regulates murine neurogenesis
Calvo CF, Fontaine RH, Soueid J, Tammela T, Makinen T, Alfaro-Cervello C, Bonnaud F, Miguez A, Benhaim L, Xu Y, Barallobre MJ, Moutkine I, Lyytikkä J, Tatlisumak T, Pytowski B, Zalc B, Richardson W, Kessaris N, Garcia-Verdugo JM, Alitalo K, Eichmann A, Thomas JL. Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis. Genes & Development 2011, 25: 831-844. PMID: 21498572, PMCID: PMC3078708, DOI: 10.1101/gad.615311.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedEnzyme-Linked Immunosorbent AssayImmunohistochemistryLymphangiogenesisMiceMice, Mutant StrainsMicroscopy, Electron, TransmissionNeovascularization, PhysiologicNeural Stem CellsNeurogenesisOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionVascular Endothelial Growth Factor Receptor-3ConceptsNeural stem cellsSubventricular zoneVEGF receptorsNeural cellsVEGFR-3Vascular endothelial growth factor (VEGF) familyEndothelial growth factor familyVascular endothelial growth factor receptor 3VEGFR-3 expressionMultipotent neural stem cellsCapillary endothelial cellsGrowth factor receptor 3Overexpression of VEGFGrowth factor familyAdult neurogenesisSVZ neurogenesisReporter miceReceptor 3NeurogenesisNeurodegenerative diseasesConditional deletionEndothelial cellsGrowth factorLigand VEGFInducible deletion