2019
Minimally Invasive Delivery of Microbeads with Encapsulated, Viable and Quiescent Neural Stem Cells to the Adult Subventricular Zone
Matta R, Lee S, Genet N, Hirschi KK, Thomas JL, Gonzalez AL. Minimally Invasive Delivery of Microbeads with Encapsulated, Viable and Quiescent Neural Stem Cells to the Adult Subventricular Zone. Scientific Reports 2019, 9: 17798. PMID: 31780709, PMCID: PMC6882840, DOI: 10.1038/s41598-019-54167-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell EncapsulationCell LineCell ProliferationCell SurvivalEndothelial CellsLateral VentriclesMaleMatrix MetalloproteinasesMiceMice, Inbred C57BLMicrospheresNeural Stem CellsNeuronsPolyethylene GlycolsRecovery of FunctionStem Cell NicheStem Cell TransplantationConceptsEndothelial cellsSubventricular zoneNSC quiescenceNon-injury modelQuiescent neural stem cellsAdult subventricular zoneNeuronal stem cellsStem cellsNeural stem cellsFunctional recoveryNeurological injuryInflammatory responseNeural stem cell maintenanceNSC deliveryNeural tissue repairNeurological diseasesMouse brainCell therapyNSC viabilityBrainTissue repairInjuryCo-encapsulated cellsSurvivalDeliveryRNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells
Ghazale H, Ripoll C, Leventoux N, Jacob L, Azar S, Mamaeva D, Glasson Y, Calvo CF, Thomas JL, Meneceur S, Lallemand Y, Rigau V, Perrin FE, Noristani HN, Rocamonde B, Huillard E, Bauchet L, Hugnot JP. RNA Profiling of the Human and Mouse Spinal Cord Stem Cell Niches Reveals an Embryonic-like Regionalization with MSX1+ Roof-Plate-Derived Cells. Stem Cell Reports 2019, 12: 1159-1177. PMID: 31031189, PMCID: PMC6524006, DOI: 10.1016/j.stemcr.2019.04.001.Peer-Reviewed Original ResearchConceptsTranscription factorsRNA profilingDevelopmental transcription factorsDorsal-ventral patternStem cell nicheEpendymal zoneMolecular resourcesMammalian lesionsConserved expressionCell nicheNeural stem cellsCell diversityPossible endogenous sourceQuiescent cellsGenesFloor plateStem cellsMsx1Endogenous sourcesTransgenic miceCellsProfilingSpinal cordCentral canalExpression
2015
Vascular Platform to Define Hematopoietic Stem Cell Factors and Enhance Regenerative Hematopoiesis
Poulos MG, Crowley MJP, Gutkin MC, Ramalingam P, Schachterle W, Thomas JL, Elemento O, Butler JM. Vascular Platform to Define Hematopoietic Stem Cell Factors and Enhance Regenerative Hematopoiesis. Stem Cell Reports 2015, 5: 881-894. PMID: 26441307, PMCID: PMC4649106, DOI: 10.1016/j.stemcr.2015.08.018.Peer-Reviewed Original ResearchConceptsBM endothelial cellsHematopoietic stem cellsBone marrowDevelopment of therapiesAdult bone marrowCytokine profileMyeloablative regimensMyeloablative irradiationStem cell factorRegenerative hematopoiesisPerivascular cellsCellular therapyEndothelial cellsDisease statesCytokine supplementationVascular platformCell factorVascular nicheTherapyPrevious reportsStem cellsEmbryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress
Ge XQ, Han J, Cheng EC, Yamaguchi S, Shima N, Thomas JL, Lin H. Embryonic Stem Cells License a High Level of Dormant Origins to Protect the Genome against Replication Stress. Stem Cell Reports 2015, 5: 185-194. PMID: 26190528, PMCID: PMC4618655, DOI: 10.1016/j.stemcr.2015.06.002.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsStem/progenitor cellsNeural stem/progenitor cellsStem cellsProgenitor cellsTissue stem/progenitor cellsMCM2-7 complexDNA replication originsTissue-specific stem/progenitor cellsStem cell typesGenome integrityGenomic integrityReplication stressDormant originsReplication forksReplicative stressDNA replicationReplication originsNeural lineagesDNA damageS phaseCell typesAbnormal neurogenesisCellsGenomeVascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans
Han J, Calvo CF, Kang TH, Baker KL, Park JH, Parras C, Levittas M, Birba U, Pibouin-Fragner L, Fragner P, Bilguvar K, Duman RS, Nurmi H, Alitalo K, Eichmann AC, Thomas JL. Vascular Endothelial Growth Factor Receptor 3 Controls Neural Stem Cell Activation in Mice and Humans. Cell Reports 2015, 10: 1158-1172. PMID: 25704818, PMCID: PMC4685253, DOI: 10.1016/j.celrep.2015.01.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell ProliferationCells, CulturedEmbryonic Stem CellsExtracellular Signal-Regulated MAP KinasesHippocampusHumansMiceMice, Inbred C57BLNeural Stem CellsNeurogenesisProto-Oncogene Proteins c-aktRecombinant ProteinsSignal TransductionVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3ConceptsHuman embryonic stem cellsNeural stem cellsVascular endothelial growth factor receptor 3Growth factor receptor 3NSC activationStem cellsProgenitor cellsAdult hippocampal neural stem cellsEmbryonic stem cellsNeural stem cell activationStem cell activationQuiescent neural stem cellsNeural progenitor cellsCell fateReceptor 3Specific regulatorsAdult mammalian hippocampusMolecular mechanismsCell cycleHippocampal neural stem cellsLigand VEGFERK pathwayConditional deletionNew neuronsVEGFR3
2012
Molecular Parallels between Neural and Vascular Development
Eichmann A, Thomas JL. Molecular Parallels between Neural and Vascular Development. Cold Spring Harbor Perspectives In Medicine 2012, 3: a006551. PMID: 23024177, PMCID: PMC3530036, DOI: 10.1101/cshperspect.a006551.Peer-Reviewed Original ResearchConceptsCentral nervous systemAdult neurogenic nichesMolecular parallelsNeural stem cellsCardiac outputMolecular mechanismsBlood vesselsNeurovascular interactionsVascular developmentHuman diseasesStem cellsCNS blood vesselsHuman central nervous systemBlood-brain barrierVascular endothelial growth factorNeurogenic nicheCell populationsEndothelial growth factorGrowth factorEndothelial cellsBlood glucoseNervous systemImportant roleCellsUnique population
2008
Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells
Delaunay D, Heydon K, Cumano A, Schwab M, Thomas J, Suter U, Nave K, Zalc B, Spassky N. Early Neuronal and Glial Fate Restriction of Embryonic Neural Stem Cells. Journal Of Neuroscience 2008, 28: 2551-2562. PMID: 18322099, PMCID: PMC6671176, DOI: 10.1523/jneurosci.5497-07.2008.Peer-Reviewed Original ResearchConceptsGlial cellsEmbryonic neural stem cellsNeuronal progenitor cellsFate restrictionRadial glial cellsEmbryonic developmentNeural stem cellsNeuroepithelial progenitorsFate mappingNeuronal precursorsNeuroepithelial cellsNeurogenic periodStem cellsClonal analysisGlial precursorsProgenitor cellsGliogenic periodCellsProteolipid proteinNew poolDifferent time pointsLater stagesEmbryogenesis
2007
VEGFR‐3 and VEGF‐C: newcomers in the neurovascular cross‐talk
Thomas J. VEGFR‐3 and VEGF‐C: newcomers in the neurovascular cross‐talk. The FASEB Journal 2007, 21: a197-a197. DOI: 10.1096/fasebj.21.5.a197-a.Peer-Reviewed Original ResearchVEGFR-3Central nervous systemVEGF-CLymphangiogenic factors VEGF-CNervous systemVEGF-C/VEGFR-3Biology of neural stem cellsEmbryonic central nervous systemMigration of neural precursorsNeural stem cellsVEGF-C/DProgenitor cellsPostnatal neurogenesisAnimal modelsBrain tumorsPostmitotic neuronsStem cellsNeural precursorsNeural proliferationNeurogenic nicheGain of function experimentsNeural cellsCross-talk
2004
Evaluation of Hematopoietic Potential Generated by Transplantation of Muscle-Derived Stem Cells in Mice
Farace F, Prestoz L, Badaoui S, Guillier M, Haond C, Opolon P, Thomas JL, Zalc B, Vainchenker W, Turhan AG. Evaluation of Hematopoietic Potential Generated by Transplantation of Muscle-Derived Stem Cells in Mice. Stem Cells And Development 2004, 13: 83-92. PMID: 15068696, DOI: 10.1089/154732804773099281.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesBone Marrow CellsBone Marrow TransplantationCell DivisionCell LineageCell SeparationCell TransplantationChimeraFemaleFlow CytometryHematopoiesisHematopoietic Stem CellsHematopoietic SystemIn Situ Hybridization, FluorescenceKiller Cells, NaturalLeukocyte Common AntigensMaleMiceMice, Inbred C57BLMusclesPolymerase Chain ReactionSex FactorsStem CellsT-LymphocytesConceptsMarrow transplantationUse of muscleStem cellsTransplantation of musclesNatural killer cellsMarrow-derived stem cellsMuscle-derived hematopoietic stem cellsLevel of CFUMuscle-derived stem cellsMarrow stem cellsMuscle tissueMuscle transplantsKiller cellsTertiary recipientsHematopoietic chimerismMuscle graftsIrradiated miceAdult miceTransplantationHematopoietic stem cellsSecondary recipientsSimilar potencyMiceMurine muscleSerial transplantation