2007
Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor
Pike JW, Meyer MB, Watanuki M, Kim S, Zella LA, Fretz JA, Yamazaki M, Shevde NK. Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 389-395. PMID: 17223545, PMCID: PMC1868541, DOI: 10.1016/j.jsbmb.2006.12.050.Peer-Reviewed Original ResearchConceptsTarget genesBasal transcriptional machineryChromatin immunoprecipitation techniqueVitamin D receptorKey target genesExpression of genesAltered gene expressionTranscriptional machineryVertebrate organismsGene regulationSystemic signalsTranscriptional modulationRegulatory regionsDNA sitesGene locusGene expressionPromoter regionEnhancer regionGenesImmunoprecipitation techniquesRegulatory capabilitiesMaintenance of calciumModular natureExpressionNew insights
2006
1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription.
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription. Endocrinology 2006, 20: 2215-30. PMID: 16613987, DOI: 10.1210/me.2006-0102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesCells, CulturedChromatinChromatin ImmunoprecipitationConserved SequenceDimerizationDNAGene Expression RegulationHumansIntronsLDL-Receptor Related ProteinsLow Density Lipoprotein Receptor-Related Protein-5MiceMice, Inbred C57BLMolecular Sequence DataOsteoblastsPromoter Regions, GeneticProtein BindingReceptors, CalcitriolRetinoid X ReceptorsRNA Polymerase IISequence AlignmentTranscription, GeneticVitamin DVitamin D Response ElementConceptsVitamin D response elementD response elementResponse elementDNA microarray analysisChromatin structureChromatin immunoprecipitationOsteoblast biologyReceptor FrizzledHuman genomeLipoprotein receptor-related protein 5Regulatory regionsStart siteLrp5 locusLow-density lipoprotein receptor-related protein 5Sequence elementsHeterologous promoterMature osteoblastsMicroarray analysisOsteoblast precursorsDirect targetProtein 5Cell line originLine originPrimary osteoblastsGenes