Comparative study of culture, next‐generation sequencing, and immunoassay for identification of pathogen in diabetic foot ulcer
Lipof JS, Jones CMC, Daiss J, Oh I. Comparative study of culture, next‐generation sequencing, and immunoassay for identification of pathogen in diabetic foot ulcer. Journal Of Orthopaedic Research® 2021, 39: 2638-2645. PMID: 33543785, PMCID: PMC8339135, DOI: 10.1002/jor.25001.Peer-Reviewed Original ResearchMeSH KeywordsDiabetes MellitusDiabetic FootHigh-Throughput Nucleotide SequencingHumansImmunoassayStaphylococcal InfectionsStaphylococcus aureusConceptsDiabetic foot infectionsMusculoskeletal infectionsNext-generation sequencingS. agalactiaeS. aureusCulture-directed antibioticsDeep musculoskeletal infectionNegative initial culturesProspective observational studyTime of diagnosisDiabetic foot ulcersLimb salvage effortsPeripheral blood samplesYears of ageFoot infectionsSurgical debridementFoot ulcersRecurrent infectionsTreatment successFair correlationIntraoperative samplesObservational studyBlood samplesUseful adjunctOrthopaedic surgeons