2018
Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis
Zhao H, Roistacher D, Helmann J. Aspartate deficiency limits peptidoglycan synthesis and sensitizes cells to antibiotics targeting cell wall synthesis in Bacillus subtilis. Molecular Microbiology 2018, 109: 826-844. PMID: 29995990, PMCID: PMC6185803, DOI: 10.1111/mmi.14078.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsAsparagineAspartate AminotransferasesAspartic AcidBacillus subtilisCell WallDiaminopimelic AcidGlutamic AcidPeptidoglycanConceptsDifco sporulation mediumPeptidoglycan synthesisCentral metabolismCell envelope stress responseEnvelope stress responseCell wall synthesisCell lysisWall synthesisSensitizes cellsStress responseAlteration of metabolismUptake systemBacillus subtilisLater stepsAmino acid analysisDiaminopimelateLB mediumSporulation mediumAcid analysisPhysiological studiesSynthesis inhibitorCompetitive inhibitorMetabolismImportant targetCells
2017
Don’t let sleeping dogmas lie: new views of peptidoglycan synthesis and its regulation
Zhao H, Patel V, Helmann J, Dörr T. Don’t let sleeping dogmas lie: new views of peptidoglycan synthesis and its regulation. Molecular Microbiology 2017, 106: 847-860. PMID: 28975672, PMCID: PMC5720918, DOI: 10.1111/mmi.13853.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsBacillus subtilisBacterial ProteinsCell WallCytoskeletal ProteinsEscherichia coliEscherichia coli ProteinsPeptidoglycanConceptsCell wall biogenesisCell wall synthesisWall biogenesisWall synthesisBacterial cell wall biogenesisCell wall synthesis processRod-shaped bacteriaBacterial cell wall synthesisSingle-molecule trackingPeptidoglycan synthesisCell wallMolecule trackingIntense research focusBiogenesisBacteriaCircumstantial evidencePowerful antibioticsFundamental questionsRegulationTechnical limitationsBiochemistryNew dataTemporal mappingSynthesisOld observations
2014
Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter
Kingston A, Zhao H, Cook G, Helmann J. Accumulation of heptaprenyl diphosphate sensitizes Bacillus subtilis to bacitracin: implications for the mechanism of resistance mediated by the BceAB transporter. Molecular Microbiology 2014, 93: 37-49. PMID: 24806199, PMCID: PMC4077933, DOI: 10.1111/mmi.12637.Peer-Reviewed Original Research