2021
Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries
Liu N, Neuenkirchen N, Zhong M, Lin H. Genome-wide mapping of Piwi association with specific loci in Drosophila ovaries. G3: Genes, Genomes, Genetics 2021, 11: jkaa059. PMID: 33609367, PMCID: PMC8022938, DOI: 10.1093/g3journal/jkaa059.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsProtein-coding genesDrosophila ovaryGermline stem cell maintenanceRole of piRNAsTermination sitesGenome-wide mappingGenomic binding profileTranscriptional termination sitesSpecific genomic sitesStem cell maintenanceRNA pathwaysTransposon repressionTranscriptional startEuchromatic regionsGene regulationEpigenetic regulationGenomic sitesCell maintenancePiwiSpecific lociMethylation signalsDiverse mechanismsTarget siteBinding sites
2020
Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis
Uyhazi KE, Yang Y, Liu N, Qi H, Huang XA, Mak W, Weatherbee SD, de Prisco N, Gennarino VA, Song X, Lin H. Pumilio proteins utilize distinct regulatory mechanisms to achieve complementary functions required for pluripotency and embryogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 7851-7862. PMID: 32198202, PMCID: PMC7148564, DOI: 10.1073/pnas.1916471117.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsTarget messenger RNAsPumilio proteinsPUM proteinsMessenger RNAEssential functionsStem cell maintenanceDistinct regulatory mechanismsEmbryonic day 8.5ESC pluripotencyTranslational regulatorPluripotency genesGene regulationEarly embryogenesisDifferentiation genesPosttranscriptional levelHigh homologyMRNA stabilityRegulatory mechanismsDouble mutant micePluripotencyDay 8.5Morula stagePluripotency markersEmbryogenesis
2019
Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea
Wang C, Yang ZZ, Guo FH, Shi S, Han XS, Zeng A, Lin H, Jing Q. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. Journal Of Biological Chemistry 2019, 294: 9873-9887. PMID: 31076507, PMCID: PMC6597837, DOI: 10.1074/jbc.ra118.004445.Peer-Reviewed Original ResearchConceptsPIWI proteinsPIWI-interacting RNA (piRNA) biogenesisPlanarian adult stem cellsHeat shock protein 40 family membersDifferent evolutionary lineagesPlanarian Schmidtea mediterraneaStem cell maintenanceStem cell regulationCo-immunoprecipitation assaysStem cellsSomatic stem cellsControl of proteinAdult stem cellsHuman gastric cancer cellsPiRNA biogenesisRNA biogenesisEvolutionary lineagesPiwi-like RNASchmidtea mediterraneaTwo-hybridSMEDWI-1Planarian speciesCell maintenanceGastric cancer cellsPlanarian regeneration
2015
Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis
Gonzalez J, Qi H, Liu N, Lin H. Piwi Is a Key Regulator of Both Somatic and Germline Stem Cells in the Drosophila Testis. Cell Reports 2015, 12: 150-161. PMID: 26119740, PMCID: PMC4497877, DOI: 10.1016/j.celrep.2015.06.004.Peer-Reviewed Original ResearchConceptsGermline stem cellsDrosophila testisSomatic cyst cellsPIWI-piRNA pathwayStem cell maintenanceCell-autonomous functionStem cellsGerm cell differentiationStem cell typesFasciclin 3GSC daughtersGermline functionSomatic rolePiwi expressionCyst cellsCell maintenanceEpigenetic mechanismsPiwiGenes keyNuclear localizationKey regulatorMolecular mechanismsCell differentiationGonadal developmentCell types
2014
PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells
Cheng EC, Kang D, Wang Z, Lin H. PIWI Proteins Are Dispensable for Mouse Somatic Development and Reprogramming of Fibroblasts into Pluripotent Stem Cells. PLOS ONE 2014, 9: e97821. PMID: 25238487, PMCID: PMC4169525, DOI: 10.1371/journal.pone.0097821.Peer-Reviewed Original ResearchConceptsPIWI proteinsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsIPS cellsPiwi genesGermline stem cell maintenanceStem cellsControl iPS cellsDifferentiated somatic cellsStem cell maintenancePIWI protein familyReprogramming of fibroblastsExpression profiling revealsGermline developmentProtein familyKnockout embryosCell maintenanceFemale fertileSomatic cellsEmbryonic fibroblastsDirect reprogrammingProfiling revealsTeratoma assayGerm layers
2011
Uniting Germline and Stem Cells: The Function of Piwi Proteins and the piRNA Pathway in Diverse Organisms
Juliano C, Wang J, Lin H. Uniting Germline and Stem Cells: The Function of Piwi Proteins and the piRNA Pathway in Diverse Organisms. Annual Review Of Genetics 2011, 45: 447-469. PMID: 21942366, PMCID: PMC3832951, DOI: 10.1146/annurev-genet-110410-132541.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsDiverse organismsStem cellsArgonaute protein familyPIWI-piRNA pathwayStem cell maintenanceSomatic stem/progenitor cellsAdult stem cellsStem/progenitor cellsPiRNA pathwayGermline specificationAnimal phylogenyGenome integrityProtein familyPosttranscriptional regulationCell maintenanceProtein bindsSomatic cellsEpigenetic programmingGermlineProgenitor cellsProteinOrganismsCommon mechanism
2009
The Biogenesis and Function of PIWI Proteins and piRNAs: Progress and Prospect
Thomson T, Lin H. The Biogenesis and Function of PIWI Proteins and piRNAs: Progress and Prospect. Annual Review Of Cell And Developmental Biology 2009, 25: 355-376. PMID: 19575643, PMCID: PMC2780330, DOI: 10.1146/annurev.cellbio.24.110707.175327.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsPIWI proteinsGermline stem cell maintenanceStem cell maintenanceSmall noncoding RNAsGermline determinationAGO proteinsGermline developmentMetazoan speciesPiRNA speciesPIWI subfamiliesSmall RNAsIntergenic sequencesPosttranscriptional regulationCell maintenanceRNA precursorsNoncoding RNAsPiwi familyDiverse functionsDiverse tissuesRNANovel mechanismProteinBiogenesisSpeciesThe Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster
Szakmary A, Reedy M, Qi H, Lin H. The Yb protein defines a novel organelle and regulates male germline stem cell self-renewal in Drosophila melanogaster. Journal Of Cell Biology 2009, 185: 613-627. PMID: 19433453, PMCID: PMC2711570, DOI: 10.1083/jcb.200903034.Peer-Reviewed Original ResearchConceptsYB proteinsNiche cellsYb bodiesElectron-dense spheresDrosophila melanogasterGermline stem cell maintenanceMale germline stem cellsGermline stem cellsStem cell maintenanceStem cellsC-terminal regionSomatic stem cellsN-terminal regionD. melanogasterNovel organellePiwi expressionGermline cellsDouble mutantCell maintenanceNovel lociSomatic cellsCytoplasmic spotsMelanogasterCytoplasmic structuresGermline loss
2003
Screens for piwi Suppressors in Drosophila Identify Dosage-Dependent Regulators of Germline Stem Cell Division
Smulders-Srinivasan TK, Lin H. Screens for piwi Suppressors in Drosophila Identify Dosage-Dependent Regulators of Germline Stem Cell Division. Genetics 2003, 165: 1971-1991. PMID: 14704180, PMCID: PMC1462913, DOI: 10.1093/genetics/165.4.1971.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedArgonaute ProteinsCell DivisionChromosomesDrosophilaDrosophila ProteinsFemaleGene DosageGene Expression Regulation, DevelopmentalGenes, LethalGerm CellsInfertility, MaleMaleProteinsRNA-Induced Silencing ComplexStem CellsSuppression, GeneticTranscription, GeneticZygoteConceptsStem cell divisionGermline stem cell divisionGenes/sequencesCell divisionFemale-specific lethalityDosage-dependent regulatorGermline stem cellsStem cell maintenanceDrosophila third chromosomeFamily of genesDosage-sensitive mannerPiwi mutantsZygotic functionMutant backgroundPiwi genesThird chromosomeMale germlineMale sterilitySuppressor mutationsSuch genesCell maintenanceGenetic regulationPlant kingdomTranscription factorsTranscriptional level