2011
Podocyte COX-2 Exacerbates Diabetic Nephropathy by Increasing Podocyte (Pro)renin Receptor Expression
Cheng H, Fan X, Moeckel GW, Harris RC. Podocyte COX-2 Exacerbates Diabetic Nephropathy by Increasing Podocyte (Pro)renin Receptor Expression. Journal Of The American Society Of Nephrology 2011, 22: 1240-1251. PMID: 21737546, PMCID: PMC3137572, DOI: 10.1681/asn.2010111149.Peer-Reviewed Original ResearchConceptsCOX-2 transgenic miceDiabetic nephropathyFoot process effacementCOX-2Transgenic miceGlomerular injuryReceptor expressionHigh glucoseRenin-angiotensin systemMesangial matrix expansionCOX-2 inhibitionWild-type miceCOX-2 inhibitorsCyclooxygenase-2 expressionGlomerular basement membraneStreptozotocin modelProgressive albuminuriaSegmental thickeningMesangial expansionDiabetic mellitusCell injuryAnimal modelsInjuryIncreased expressionNephropathy
2009
Distinct Roles for Basal and Induced COX-2 in Podocyte Injury
Cheng H, Fan X, Guan Y, Moeckel GW, Zent R, Harris RC. Distinct Roles for Basal and Induced COX-2 in Podocyte Injury. Journal Of The American Society Of Nephrology 2009, 20: 1953-1962. PMID: 19643929, PMCID: PMC2736764, DOI: 10.1681/asn.2009010039.Peer-Reviewed Original ResearchMeSH KeywordsAlbuminuriaAnimalsAntibiotics, AntineoplasticApoptosisCell AdhesionCell Line, TransformedCell SurvivalCyclooxygenase 2DinoprostoneDoxorubicinGlomerulonephritisMaleMiceMice, Inbred StrainsMice, TransgenicPodocytesPuromycinReceptors, Prostaglandin EReceptors, ThromboxaneRNA, MessengerThromboxanesConceptsCyclooxygenase-2Thromboxane receptorCOX-2 knockout miceSelective deletionCOX-2 deletionInduced COX-2Receptor subtype 4COX-2 metabolitesFoot process effacementGlomerular injuryPodocyte injuryProstanoid receptorsAttenuated albuminuriaWild-type podocytesSubtype 4Transgenic miceProcess effacementTP antagonistPodocyte survivalInjuryMore prostaglandinsGenetic deletionMicePodocytesGreater expression
2007
Puromycin Induces Reversible Proteinuric Injury in Transgenic Mice Expressing Cyclooxygenase-2 in Podocytes
Jo YI, Cheng H, Wang S, Moeckel GW, Harris RC. Puromycin Induces Reversible Proteinuric Injury in Transgenic Mice Expressing Cyclooxygenase-2 in Podocytes. Nephron 2007, 107: e87-e94. PMID: 17890881, DOI: 10.1159/000108653.Peer-Reviewed Original ResearchConceptsCOX-2 expressionCOX-2-specific inhibitorsCOX-2 mRNATransgenic miceFoot process effacementDay 3COX-2Process effacementEndogenous COX-2 mRNAImmunoreactive COX-2Progressive renal diseaseCOX-2 overexpressionFoot process fusionCyclooxygenase-2 inhibitorRenal diseaseGlomerular injurySignificant albuminuriaCyclooxygenase-2Further injuryReal-time PCRDay 1Nephrin mRNADay 10AlbuminuriaMiceOverexpression of Cyclooxygenase-2 Predisposes to Podocyte Injury
Cheng H, Wang S, Jo YI, Hao CM, Zhang M, Fan X, Kennedy C, Breyer MD, Moeckel GW, Harris RC. Overexpression of Cyclooxygenase-2 Predisposes to Podocyte Injury. Journal Of The American Society Of Nephrology 2007, 18: 551-559. PMID: 17202413, DOI: 10.1681/asn.2006090990.Peer-Reviewed Original ResearchConceptsCOX-2 expressionBALB/c miceFoot process effacementTransgenic miceC micePodocyte injuryProcess effacementCOX-2 transgenic miceImmunoreactive COX-2 expressionCOX-2 mRNA expressionNephrin promoterLong-term treatmentCOX-2 overexpressionTransgenic mouse kidneyCyclooxygenase-2 expressionWild-type miceWild-type littermatesCOX-2 mRNARenal injuryRenal ablationAdriamycin nephropathyMore albuminuriaAdriamycin administrationFurther injuryCOX-2
2003
COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*
Moeckel GW, Zhang L, Fogo AB, Hao CM, Pozzi A, Breyer MD. COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*. Journal Of Biological Chemistry 2003, 278: 19352-19357. PMID: 12637551, DOI: 10.1074/jbc.m302209200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAldehyde ReductaseAnimalsApoptosisBetaineCaspase 3CaspasesCell SurvivalCells, CulturedCyclooxygenase 2Cyclooxygenase 2 InhibitorsCyclooxygenase InhibitorsDehydrationDNA FragmentationGene ExpressionHeat-Shock ProteinsHypertonic SolutionsIn Situ Nick-End LabelingInositolIsoenzymesKidney MedullaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutProstaglandin-Endoperoxide SynthasesRNA, MessengerSorbitolSymportersTritiumConceptsMedullary interstitial cellsRenal medullary interstitial cellsInterstitial cellsCOX2 activityCOX2 inhibitionReductase mRNA expressionCOX2-specific inhibitorsRenal cell survivalCell survivalAnalgesic nephropathyCOX2 inhibitorsRenal medullaMRNA expressionPmol/Sorbitol accumulationInner medullaOrganic osmolyte accumulationKidney inner medullaMedullaCell deathBetaine concentrationsHypertonic stressPresent studySurvivalInhibitors
2002
Cyclooxygenase-2 inhibitor blocks expression of mediators of renal injury in a model of diabetes and hypertension1
Cheng HF, Wang CJ, Moeckel GW, Zhang MZ, Mckanna JA, Harris RC. Cyclooxygenase-2 inhibitor blocks expression of mediators of renal injury in a model of diabetes and hypertension1. Kidney International 2002, 62: 929-939. PMID: 12164875, DOI: 10.1046/j.1523-1755.2002.00520.x.Peer-Reviewed Original ResearchConceptsCOX-2 expressionCOX-2 inhibitorsDiabetic groupRenal injuryDiabetic ratsImmunoreactive COX-2 expressionCortical thick ascending limbSelective COX-2 inhibitorsSystolic blood pressureModel of diabetesVascular endothelial growth factorDevelopment of glomerulosclerosisCOX-2 inhibitionThick ascending limbEndothelial growth factorMesangial sclerosis indexTubulointerstitial injuryDiabetic nephropathyBlood pressureChronic treatmentSubtotal nephrectomySclerosis indexBlood glucoseMacula densaRenal cortex