2024
Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain
Gisriel C, Kurashov V, Iwig D, Russell B, Vinyard D, Brudvig G, Golbeck J, Lakshmi K. Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain. Science Advances 2024, 10: eadp4937. PMID: 39612342, PMCID: PMC11606441, DOI: 10.1126/sciadv.adp4937.Peer-Reviewed Original ResearchConceptsCryo-EM structureChemical bond energyElectron transfer chainBond energyPCC 6803Fuel catalystPhotosystem IBinding environmentTransfer chainLow binding affinityI variantsPlastoquinone derivativesBinding affinityCatalystPlastoquinone-9PS ICyanobacteriumBiophysical dataPhotosystemEnergyPhotonsVariantsAffinityStructure of a biohybrid photosystem I-platinum nanoparticle solar fuel catalyst
Gisriel C, Malavath T, Qiu T, Menzel J, Batista V, Brudvig G, Utschig L. Structure of a biohybrid photosystem I-platinum nanoparticle solar fuel catalyst. Nature Communications 2024, 15: 9519. PMID: 39496605, PMCID: PMC11535483, DOI: 10.1038/s41467-024-53476-y.Peer-Reviewed Original ResearchLight-driven H2 productionFuel catalystUnity quantum efficiencyPhotosynthetic biohybrid systemsPigment-protein complexesLight-driven enzymePlatinum nanoparticlesH2 productionQuantum efficiencyPhotosystem I complexCatalystChemical energyCryo-EM structureResolution cryo-EM structureBiohybrid systemsFuel productionStored solar energyPhotosystem IBiology platformReducing equivalentsMolecular basisI complexSolar energyBinding sitesStructure
2002
Electronic Structure of the P700 Special Pair from High-Frequency Electron Paramagnetic Resonance Spectroscopy
Poluektov O, Utschig L, Schlesselman S, Lakshmi K, Brudvig G, Kothe G, Thurnauer M. Electronic Structure of the P700 Special Pair from High-Frequency Electron Paramagnetic Resonance Spectroscopy. The Journal Of Physical Chemistry B 2002, 106: 8911-8916. DOI: 10.1021/jp021465+.Peer-Reviewed Original ResearchElectron paramagnetic resonance spectroscopyParamagnetic resonance spectroscopyTriplet stateFrequency Electron Paramagnetic Resonance SpectroscopyElectronic structureHigh-frequency electron paramagnetic resonance spectroscopyResonance spectroscopyPrimary donorPrimary donor cationPhotosynthetic reaction centersHigh-frequency EPRDonor cationI. Electronic structureElectronic characterReaction centersValue anisotropySpecial pairTensor axesCationsSpectroscopyPhotosystem IP700T stateEPRStructureElectron Paramagnetic Resonance Distance Measurements in Photosystems
Lakshmi K, Brudvig G. Electron Paramagnetic Resonance Distance Measurements in Photosystems. Biological Magnetic Resonance 2002, 19: 513-567. DOI: 10.1007/0-306-47109-4_12.Peer-Reviewed Original ResearchEPR distance measurementsDistance measurementsElectron Paramagnetic Resonance Distance MeasurementsSpin-lattice relaxation measurementsPhotosynthetic reaction centersDistance measurement techniqueLine shape analysisInterspin distancesEPR studiesRedox cofactorsRelaxation measurementsSingle crystalsSuch measurementsReaction centersPhotosystem IIPhotosystem IMeasurement techniquesBiological systemsRelative orientationMeasurementsCWCrystalsShape analysisPhotosystemsCofactor
2001
Direct electrochemistry of photosystem I
Kievit O, Brudvig G. Direct electrochemistry of photosystem I. Journal Of Electroanalytical Chemistry 2001, 497: 139-149. DOI: 10.1016/s0022-0728(00)00467-8.Peer-Reviewed Original ResearchDirect electrochemistryPhotosystem I.Primary electron donorElectrochemical signalPeak separationReduction potentialChlorophyll dimerElectron donorSolubilized membrane proteinsRange of proteinsElectrochemistryPresence of detergentPhotosystem ILiterature valuesDimersPowerful toolSeparationP700Membrane proteinsDetergents
1991
A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes
Miller A, Brudvig G. A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes. Biochimica Et Biophysica Acta 1991, 1056: 1-18. PMID: 1845842, DOI: 10.1016/s0005-2728(05)80067-2.Peer-Reviewed Original ResearchConceptsElectron paramagnetic resonance spectroscopyElectron paramagnetic resonance signalParamagnetic resonance spectroscopyParamagnetic resonance signalPhotosystem II membranesPhotosynthetic electron transportResonance spectroscopyResonance signalsPhotosystem IIBacterial reaction centersElectron transportParamagnetic speciesCytochrome b6fPhotosystem IBiochemical basisReaction centersSpectroscopySpeciesSpectral featuresCommon contaminantsMembraneMoietySeminal experimentsB6fCofactor