2016
Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins
Jensen KH, Brandt SS, Shigematsu H, Sigworth FJ. Statistical modeling and removal of lipid membrane projections for cryo-EM structure determination of reconstituted membrane proteins. Journal Of Structural Biology 2016, 194: 49-60. PMID: 26835990, PMCID: PMC4866491, DOI: 10.1016/j.jsb.2016.01.012.Peer-Reviewed Original Research
1999
Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow
Baumgartner W, Islas L, Sigworth F. Two-Microelectrode Voltage Clamp of Xenopus Oocytes: Voltage Errors and Compensation for Local Current Flow. Biophysical Journal 1999, 77: 1980-1991. PMID: 10512818, PMCID: PMC1300479, DOI: 10.1016/s0006-3495(99)77039-6.Peer-Reviewed Original Research
1998
Activation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 295-311. PMID: 9450945, PMCID: PMC2222768, DOI: 10.1085/jgp.111.2.295.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 271-294. PMID: 9450944, PMCID: PMC2222764, DOI: 10.1085/jgp.111.2.271.Peer-Reviewed Original ResearchActivation of Shaker Potassium Channels
Schoppa N, Sigworth F. Activation of Shaker Potassium Channels. The Journal Of General Physiology 1998, 111: 313-342. PMID: 9450946, PMCID: PMC2222769, DOI: 10.1085/jgp.111.2.313.Peer-Reviewed Original Research
1997
Enhanced Closed-state Inactivation in a Mutant Shaker K+ Channel
Ayer, Jr. R, Sigworth F. Enhanced Closed-state Inactivation in a Mutant Shaker K+ Channel. The Journal Of Membrane Biology 1997, 157: 215-230. PMID: 9178609, DOI: 10.1007/s002329900230.Peer-Reviewed Original Research
1996
Impaired slow inactivation in mutant sodium channels
Cummins T, Sigworth F. Impaired slow inactivation in mutant sodium channels. Biophysical Journal 1996, 71: 227-236. PMID: 8804606, PMCID: PMC1233474, DOI: 10.1016/s0006-3495(96)79219-6.Peer-Reviewed Original ResearchConceptsRat skeletal muscle channelsSodium currentProlonged depolarizationSlow inactivationPersistent sodium currentMaximal sodium currentSkeletal muscle channelsMuscle paralysisSkeletal muscle fibersMutant sodium channelsSustained currentHyperkalemic periodic paralysisM mutationPeriodic paralysisSodium channelsMuscle channelsMuscle fibersParalysisFast inactivationWild-type channelsMutant channelsDepolarization
1995
Design of the EPC-9, a computer-controlled patch-clamp amplifier. 2. Software
Sigworth F, Affolter H, Neher E. Design of the EPC-9, a computer-controlled patch-clamp amplifier. 2. Software. Journal Of Neuroscience Methods 1995, 56: 203-215. PMID: 7538621, DOI: 10.1016/0165-0270(94)00129-5.Peer-Reviewed Original Research
1994
Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons
Jiang C, Sigworth F, Haddad G. Oxygen deprivation activates an ATP-inhibitable K+ channel in substantia nigra neurons. Journal Of Neuroscience 1994, 14: 5590-5602. PMID: 8083755, PMCID: PMC6577106, DOI: 10.1523/jneurosci.14-09-05590.1994.Peer-Reviewed Original ResearchConceptsSubstantia nigra neuronsRat substantia nigra neuronsEffects of hypoxiaGlibenclamide binding sitesEffect of ATPCell-attached patchesNeuronal survivalKATP channelsResult of Ca2Different subtypesOutward currentsNeuronal structuresFree Ca2HypoxiaMetabolic stressNeuronsDeleterious changesSingle-channel currentsChannel activityO2 deprivationInternal ATPOxygen deprivationActivationHigh Ca2Membrane potentialVoltage gating of ion channels
Sigworth F. Voltage gating of ion channels. Quarterly Reviews Of Biophysics 1994, 27: 1-40. PMID: 7520590, DOI: 10.1017/s0033583500002894.Peer-Reviewed Original ResearchConceptsVoltage-gated calcium channelsNerve action potentialsVoltage-gated sodium channelsRelease of hormonesIon channelsVoltage-gated ion channelsCalcium channelsAction potentialsNeurotransmitter releaseMuscle contractionSodium channelsMembrane proteinsCellular signalsVoltage gatingCentral roleHormoneRelease
1993
Substitution of a hydrophobic residue alters the conformational stability of Shaker K+ channels during gating and assembly
McCormack K, Lin L, Sigworth F. Substitution of a hydrophobic residue alters the conformational stability of Shaker K+ channels during gating and assembly. Biophysical Journal 1993, 65: 1740-1748. PMID: 8274662, PMCID: PMC1225901, DOI: 10.1016/s0006-3495(93)81202-5.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiophysical PhenomenaBiophysicsDNA, ComplementaryDrosophilaDrug StabilityFemaleIon Channel GatingMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesPeptidesPotassium ChannelsProtein ConformationShaker Superfamily of Potassium ChannelsThermodynamicsXenopus laevisConceptsUncharged amino acid residuesLeucine heptad repeatWild-type subunitsPosition 370Large hydrophobic residuesAmino acid residuesSequence motifsConservative substitutionsHeptad repeatHydrophobic residuesVoltage-gated channelsLeucine residuesAcid residuesTertiary structureS4 segmentSpecific hydrophobic interactionsHydrophilic residuesResidue altersChannel subunitsInactivation gatingChannel complexSubunitsConformational stabilityConformational transitionResiduesFunctional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells.
Cascio M, Schoppa N, Grodzicki R, Sigworth F, Fox R. Functional expression and purification of a homomeric human alpha 1 glycine receptor in baculovirus-infected insect cells. Journal Of Biological Chemistry 1993, 268: 22135-22142. PMID: 8408073, DOI: 10.1016/s0021-9258(20)80658-9.Peer-Reviewed Original ResearchConceptsInsect cellsGlyR proteinSf9 cellsMembrane protein channelsSf9 insect cellsSodium dodecyl sulfate-polyacrylamide gelsDodecyl sulfate-polyacrylamide gelsSulfate-polyacrylamide gelsWhole cell lysatesHuman kidney cell lineShares sequenceKidney cell lineTransient expressionExpression systemStructural homologyChannel proteinsBiophysical characterizationProtein channelsFunctional channelsFunctional expressionMembrane fractionNative channelsCell surfaceGlycine receptorsChloride channelsFluctuations in ion channel gating currents. Analysis of nonstationary shot noise
Crouzy S, Sigworth F. Fluctuations in ion channel gating currents. Analysis of nonstationary shot noise. Biophysical Journal 1993, 64: 68-76. PMID: 8381683, PMCID: PMC1262303, DOI: 10.1016/s0006-3495(93)81341-9.Peer-Reviewed Original Research
1992
Tandem linkage of Shaker K+ channel subunits does not ensure the stoichiometry of expressed channels
McCormack K, Lin L, Iverson L, Tanouye M, Sigworth F. Tandem linkage of Shaker K+ channel subunits does not ensure the stoichiometry of expressed channels. Biophysical Journal 1992, 63: 1406-1411. PMID: 1477286, PMCID: PMC1261445, DOI: 10.1016/s0006-3495(92)81703-4.Peer-Reviewed Original Research[52] Analysis of nonstationary single-channel currents
Sigworth F, Zhou J. [52] Analysis of nonstationary single-channel currents. Methods In Enzymology 1992, 207: 746-762. PMID: 1382211, DOI: 10.1016/0076-6879(92)07054-r.Peer-Reviewed Original Research
1991
Patch recordings from the electrocytes of Electrophorus electricus. Na currents and PNa/PK variability.
Shenkel S, Sigworth F. Patch recordings from the electrocytes of Electrophorus electricus. Na currents and PNa/PK variability. The Journal Of General Physiology 1991, 97: 1013-1041. PMID: 1650809, PMCID: PMC2216506, DOI: 10.1085/jgp.97.5.1013.Peer-Reviewed Original ResearchChanges in channel properties of acetylcholine receptors during the time course of thiol chemical modifications
Bouzat C, Barrantes F, Sigworth F. Changes in channel properties of acetylcholine receptors during the time course of thiol chemical modifications. Pflügers Archiv - European Journal Of Physiology 1991, 418: 51-61. PMID: 2041725, DOI: 10.1007/bf00370451.Peer-Reviewed Original ResearchConceptsSingle acetylcholine receptor channelsHigh agonist concentrationsPatch-clamp techniqueOpen timeConcentration of NEMSilent periodAcetylcholine receptorsN-ethylmaleimideAcetylcholine receptor channelsAgonist concentrationsChannel open probabilityReceptor channelsAChRMM N-ethylmaleimideTime of exposureRate of occurrenceSingle-channel currentsTime courseNEM treatmentControl receptorsReceptorsSignificant changesTreatmentShort openingsSlight reduction
1990
Activation of Torpedo acetylcholine receptors expressed in mouse fibroblasts. Single channel current kinetics reveal distinct agonist binding affinities.
Sine S, Claudio T, Sigworth F. Activation of Torpedo acetylcholine receptors expressed in mouse fibroblasts. Single channel current kinetics reveal distinct agonist binding affinities. The Journal Of General Physiology 1990, 96: 395-437. PMID: 1698917, PMCID: PMC2228994, DOI: 10.1085/jgp.96.2.395.Peer-Reviewed Original Research
1989
Purified, modified eel sodium channels are active in planar bilayers in the absence of activating neurotoxins.
Shenkel S, Cooper E, James W, Agnew W, Sigworth F. Purified, modified eel sodium channels are active in planar bilayers in the absence of activating neurotoxins. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 9592-9596. PMID: 2556720, PMCID: PMC298544, DOI: 10.1073/pnas.86.23.9592.Peer-Reviewed Original ResearchPrimary structure and functional expression of a mammalian skeletal muscle sodium channel
Trimmer J, Cooperman S, Tomiko S, Zhou J, Crean S, Boyle M, Kalen R, Sheng Z, Barchi R, Sigworth F, Goodman R, Agnew W, Mandel G. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 1989, 3: 33-49. PMID: 2559760, DOI: 10.1016/0896-6273(89)90113-x.Peer-Reviewed Original ResearchConceptsAlpha subunitHomologous domains IAmino acid segmentSodium channelsSkeletal muscle sodium channelsNorthern blot analysisSkeletal muscleMuscle sodium channelsVoltage-sensitive sodium channelsPrimary structureFunctional expressionDomain IAcid segmentNative channelsSynthetic RNAXenopus oocytesRat muscle fibersBlot analysisRat skeletal muscleEel electroplaxRat brainSubunitsMuscle fibersMuscleCDNA