2021
Microinjection of Xenopus tropicalis Embryos.
Lane M, Mis EK, Khokha MK. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harbor Protocols 2021, 2022: pdb.prot107644. PMID: 34244348, DOI: 10.1101/pdb.prot107644.Peer-Reviewed Original ResearchConceptsEmbryo sizeLarger embryo sizeTargeted gene manipulationEmbryo development timeSmall embryo sizeXenopus tropicalis embryosDevelopmental biologyEarly embryosMicroinjection protocolGene manipulationGenetic studiesFirst divisionXenopusEmbryosImportant modelDevelopment timeMicroinjectionMorpholinoCRISPRBiologySpeciesDNAMRNAOocytesFertilization
2014
Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans
Fernandez A, Mis E, Lai A, Mauro M, Quental A, Bock C, Piano F. Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 2014, 4: 185-196. PMID: 24281427, PMCID: PMC3887534, DOI: 10.1534/g3.113.008532.Peer-Reviewed Original ResearchConceptsMEL-28Caenorhabditis elegansLethal genesGenetic interaction screensGenome-scale screeningWild-type wormsNuclear envelope functionsCell-matrix attachmentWild-type larvaeDynactin functionGenetic interactorsGenome-scaleVesicle transportConserved proteinsChromosome segregationGene networksIdentified genesCellular processesPostembryonic rolesInteraction screenNuclear poresPleiotropic functionsGenesEarly embryosLarval development